Effects of viscosity on sperm motility studied with optical tweezers

Hyun, Nicholas; Chandsawangbhuwana, Charlie; Zhu, Qingyuan; Shi, Linda Z.; Yang-Wong, Collin; Berns, Michael W.

doi:10.1117/1.jbo.17.2.025005

Cited by 21 publications

(24 citation statements)

References 24 publications

(28 reference statements)

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“…As the viscosity increased, the sperm had to generate more force to progress forward. Hyun et al [25] measured the sperm swimming force using optical tweezers. They indicated that the swimming force increased as the viscosity increased.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, they indicated that the mucus is a non-Newtonian fluid. There were several experimental studies on the effect of the viscosity on the motion characteristics of sperm [22]- [25]. Therefore, it is necessary to observe sperm motility in a high-viscosity fluid and not in a dilute fluid to understand the essential mechanism of sperm motility.…”

Section: Introductionmentioning

confidence: 99%

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Effect of viscosity on motion characteristics of bovine sperm

2015

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Abstract-Bovine sperm motion in environments of various viscosities is studied. We used the semen of Japanese cattle and investigated the following parameters: the sperm velocity, the straight-line velocity, and the amplitude from the observed sperm trajectory. As the viscosity increased, the motility of the sperm decreased. On the other hand, the power expended by the sperm flagellum was of the same order of magnitude over the viscosity range of 0.0007-0.0226 Pa·s. Additionally, the increase in the viscosity brought about a change in the flagellum shape and an increase in the percentage of sperm with a nonrotating head. The existence of rotation caused a change in the sperm velocity, amplitude, and frequency of the flagellum. These results suggest that bovine sperm has evolved to swim effectively in the oviduct, which is a highviscosity environment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of viscosity on motion characteristics of bovine sperm

2015

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“…In previous studies (5,16,17), different levels of viscosity were achieved by the addition of Ficoll, carboxymethyl cellulose, methylcellulose or EY. The results of the present study demonstrates that addition of GA could also alter the movement pattern of spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Effect of Gum Arabic on Stallion Sperm Survival During Cold Storage and Post Freezing

Ali

Musa

Alfadul

et al. 2018

Macedonian Veterinary Review

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This study is aimed at investigating effects of supplementation of stallion' semen extender with various concentrations of Gum Arabic (GA) versus egg yolk (EY) on viscosity, sperm motility and survival during cooling and freezing. Physical sperm characteristics; i.e. curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN) and straightness index (STR) were evaluated. Based on the sperm velocity (velocity of the average path), individual spermatozoons were classified into two major groups; i.e., progressively motile (>45 µm/sec) and immotile (0-45 µm/sec) spermatozoa. Addition of 3, 9 or 15% of GA to HF-20 extender resulted in linear decreases in VCL, VSL and VAP and a decrease in the percentage of progressively motile spermatozoa. Dilution of horse semen samples with high viscosityextenders (i.e., high percentage of GA) decreased the VCL, VSL and VAP in fresh and chilled semen. Freezing semen in high viscosity-extenders reduced percentage of progressively motile spermatozoa compared with those of low viscosity-extenders. In refrigerated and frozen semen samples, the extender containing 15% GA had detrimental effects on the percentage of progressively motile sperm cells and velocity of progressive motile sperm. Moreover, cooling sperm in extenders containing 9 or 15% of GA for 72 hours resulted in complete motility cessation. In conclusion, GA could replace EY in stallion semen extenders at a level of 3% to maintain the physical and biological characteristics of cold and frozen semen.

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“…In 2012, Hyun et al. investigated the effects of viscosity on human sperm motility . The viscosity of the HEPES‐buffered mHTF with 5% SSS was varied between 1 and 15 cP, similar to native viscosities of the human male reproductive tract, by addition of methylcellulose (0.5–2%).…”

Section: Measurements In Complex Mediamentioning

confidence: 99%

Strategies for Optical Trapping in Biological Samples: Aiming at Microrobotic Surgeons

Bunea

Glückstad

2019

Laser & Photonics Reviews

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Optical trapping and manipulation of objects down to the Ångstrom level has revolutionized research at the smallest scales in all natural sciences. The flexibility of optical trapping methods facilitates real‐time monitoring of the dynamics of biological processes in model systems and even in living cells. Different optical trapping and manipulation approaches allow displacement of nanostructures with subnanometer precision and force measurements with femtonewton precision. Due to inherent constraints of optical methods, most optical trapping experiments are performed in water or simple aqueous solutions. However, in recent years, there is an ever‐growing interest of shifting from simple aqueous media towards more biologically‐relevant media. Precise optical trapping and manipulation, combined with state‐of‐the‐art microfabrication, will enable the development of microrobotic “surgeons” with tremendous potential for biomedical and microengineering applications. This review introduces the basics of optical trapping and discusses its applications for biological samples, with focus on trapping in biological media and strategies for overcoming the challenges of optical manipulation in complex environments as a stepping‐stone for microrobotic “surgeons.”

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Effects of viscosity on sperm motility studied with optical tweezers

Cited by 21 publications

References 24 publications

Effect of viscosity on motion characteristics of bovine sperm

Effect of viscosity on motion characteristics of bovine sperm

Effect of Gum Arabic on Stallion Sperm Survival During Cold Storage and Post Freezing

Strategies for Optical Trapping in Biological Samples: Aiming at Microrobotic Surgeons

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