Effects of vascular endothelial growth factor (VEGF) on MC3T3-E1

Tan, Yingying; Yang, Yayuan; Chai, Lei; Wong, Ricky W.K.; Rabie, A. Bakr M.

doi:10.1111/j.1601-6343.2010.01498.x

Cited by 23 publications

(38 citation statements)

References 27 publications

(34 reference statements)

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“…OCN, a non-collagenous protein, is usually expressed during the late stages of osteogenic/odontogenic differentiation (7,14). In this study, the expression of ALP and OCN was significantly enhanced in the DPSCs/VEGF as compared with that in the DPSCs/Vector.…”

Section: A B Cmentioning

confidence: 47%

“…ALP has been widely used as a marker for differentiated cells producing mineralized matrix (7,14). OCN, a non-collagenous protein, is usually expressed during the late stages of osteogenic/odontogenic differentiation (7,14).…”

Section: A B Cmentioning

confidence: 99%

“…A previous study demonstrated that recombinant human VEGF improved the cell proliferation and alkaline phosphatase (ALP) expression of dental pulp cells (6). VEGF culture medium also promoted the osteogenic differentiation capacity of MC3T3 stem cells by increasing the expression of ALP and osteocalcin (OCN) in vitro (7). Compared with control cells, VEGF-treated human periodontal ligament stem cells exhibited a significantly higher ALP activity, which further led to the formation of increased mineralized tissue (8).…”

Section: Introductionmentioning

confidence: 99%

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Odontogenic differentiation of vascular endothelial growth factor-transfected human dental pulp stem cells in vitro

Zhang

Liu

Zhao

et al. 2014

Molecular Medicine Reports

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Section: A B Cmentioning

confidence: 47%

Section: A B Cmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Odontogenic differentiation of vascular endothelial growth factor-transfected human dental pulp stem cells in vitro

Zhang

Liu

Zhao

et al. 2014

Molecular Medicine Reports

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“…In addition, ozone gas is known to promote the production of FGF and other related cytokines 28) . VEGF enhances ALP expression in the mouse osteoblast cell line MC3T3-1 29) . Thus, it is possible that bone matrix secretion may have been indirectly promoted via the secretion of these growth factors.…”

Section: Pao-li Wang Et Almentioning

confidence: 99%

The Effect of Ozone Gel on Bone Matrix Production by Human Osteosarcoma Cell Line Saos-2

Wang

Tachi

Masuno

et al. 2018

J. Hard Tissue Biology.

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Ozone is currently being considered as a potential oral antiseptic agent because it is highly antimicrobial and does not induce microbial resistance. In this study, we demonstrated that an optimal dosage of ozone gel enhanced the proliferation, type 1 collagen production, and alkaline phosphatase (ALP) secretion of Saos-2 cells in vitro. Proliferation of Saos-2 cells was assessed by MTT and DNA synthesis assays. Type 1 collagen production and ALP secretion were evaluated using enzymelinked immunosorbent assay (ELISA) and ALP assays. The cells were treated with/without 0.05, 0.5, 5 ppm ozone gel for 24 h. Ozone gel (0.5 ppm) signifi cantly induced the proliferation of Saos-2 cells. At this concentration, ozone gel enhanced type 1 collagen production and ALP secretion. The results indicated that ozone gel controls the cellular metabolism of osteoblasts, resulting in the secretion of early bone-related biomarkers.

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“…The initial step of bone remodeling is removal of 'old' bone to provide places for new bone formation [8]. Osteoblasts play an essential role in osteogenesis, and are also responsible for differentiation and maturation of osteoclasts [9,10].…”

Section: Initial Removal Of Old Bonementioning

confidence: 99%

Effects of VEGF on Bone Cells’ Metabolism and Activity

Yang¹,

Chai²,

Liao³

et al. 2014

Dentistry

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The treatments of bone loss have been extended to the molecular level and previous research has found that vascular endothelia growth factor (VEGF) can enhance the vascular network building around bone injury to aid healing progress. In this manuscript, we discussed the effects of VEGF on bone cells' metabolism and activity. VEGF could enhance osteogenesis by inducing neovascularization and direct effects on bone cells via regulation on genes osteoprotegerin (OPG)/the receptor activator of nuclear factor kappa beta ligand (RANKL), alkaline phosphatase (ALP), osteopontin (OPN) and osteocalcin (OCN), without causing abnormal cell metabolism, which may shed lights on new clinical treatments of bone loss.

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Effects of vascular endothelial growth factor (VEGF) on MC3T3-E1

Abstract: VEGF promotes bone remodeling by direct effects on osteoblastic cells via regulating gene expression of ALP, OCN, and OPG through VEGFR2 signaling pathway.

Cited by 23 publications

References 27 publications

Odontogenic differentiation of vascular endothelial growth factor-transfected human dental pulp stem cells in vitro

Odontogenic differentiation of vascular endothelial growth factor-transfected human dental pulp stem cells in vitro

The Effect of Ozone Gel on Bone Matrix Production by Human Osteosarcoma Cell Line Saos-2

Effects of VEGF on Bone Cells’ Metabolism and Activity

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