Effects of various metal substrata on accumulation of<i>Pseudomonas aeruginosa</i>biofilms and the efficacy of monochloramine as a biocide

Chen, Ching‐I; Griebe, Thomas; Srinivasan, Rohini; Stewart, Philip S.

doi:10.1080/08927019309386256

Cited by 21 publications

(5 citation statements)

References 15 publications

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“…Videla et al (1991) reported that the biocidal activity of glutaraldehyde depended markedly on the nature of the substratum on which the biofilm was formed. Using stainless steel, mild steel and copper, Chen et al (1993) demonstrated that the efficacy of monochloroamine against Pseudomonas biofilm varied on different metal substrata. Interaction between biocides and metabolic products of SRB was also reported to affect biocide efficiency.…”

Section: Discussionmentioning

confidence: 99%

The use of biocides to control sulphate‐reducing bacteria in biofilms on mild steel surfaces

Cheung

Beech

1996

Biofouling

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Three different types of biocides, viz. formaldehyde (FM), glutaraldehyde (GA) and isothiozolone (ITZ) were used to control planktonic and sessile populations of two marine isolates of sulphate-reducing bacteria (SRB). The influence of these biocides on the initial attachment of cells to mild steel surfaces, on subsequent biofilm formation and on the activity of hydrogenase enzymes within developed biofilms was evaluated. In the presence of biocides the rate and degree of colonization of mild steel by SRB depended on incubation time, bacterial isolate and the type of biocide used. Although SRB differed in their susceptibility to biocides, for all isolates the biofilm population was more resistant to the treatment than the planktonic population. GA showed highest efficiency in controlling planktonic and sessile SRB compared with the other two biocides. The activity of the enzyme hydrogenase measured in SRB biofilms varied between isolates and with the biocide treatment. No correlation was found between the number of sessile cells and hydrogenase activity.

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Section: Discussionmentioning

confidence: 99%

The use of biocides to control sulphate‐reducing bacteria in biofilms on mild steel surfaces

Cheung

Beech

1996

Biofouling

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“…Buna rubber was shown to have an independent bacteriostatic effect. Biofilms formed on mild steel, in which some corrosion of the metal was evident, were less susceptible to killing by monochloramine than biofilms on stainless steel (9). These examples suggest that the substratum material is most likely to influence biofilm susceptibility when it leaches or corrodes.…”

Section: Factors Influencing Biofilm Susceptibilitymentioning

confidence: 99%

Antimicrobial Tolerance in Biofilms

2015

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The tolerance of microorganisms in biofilms to antimicrobial agents is examined through a meta-analysis of literature data. A numerical tolerance factor comparing the rates of killing in the planktonic and biofilm states is defined to provide a quantitative basis for the analysis. Tolerance factors for biocides and antibiotics range over three orders of magnitude. This variation is not explained by taking into account the molecular weight of the agent, the chemistry of the agent, the substratum material, or the speciation of the microorganisms. Tolerance factors do depend on the areal cell density of the biofilm at the time of treatment and on the age of the biofilm as grown in a particular experimental system. This suggests that there is something that happens during biofilm maturation, either physical or physiological, that is essential for full biofilm tolerance. Experimental measurements of antimicrobial penetration times in biofilms range over orders of magnitude, with slower penetration (>12 min) observed for reactive oxidants and cationic molecules. These agents are retarded through the interaction of reaction, sorption, and diffusion. The specific physiological status of microbial cells in a biofilm contributes to antimicrobial tolerance. A conceptual framework for categorizing physiological cell states is discussed in the context of antimicrobial susceptibility. It is likely that biofilms harbor cells in multiple states simultaneously (e.g., growing, stress-adapted, dormant, inactive) and that this physiological heterogeneity is an important factor in the tolerance of the biofilm state.

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“…We chose to work with a binary population biofilm because thicker biofilms, more like those encountered in actual fouling environments, develop with the combination of microorganisms than with either one in pure culture (11). The reactor system and operating conditions have been described elsewhere (1,2,11). Biofilm was sampled from removable stainless steel slides that fit into grooved recessions in the reactor body.…”

mentioning

confidence: 99%

Comparison of respiratory activity and culturability during monochloramine disinfection of binary population biofilms

Stewart

Griebe

Srinivasan

et al. 1994

Appl Environ Microbiol

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Biofilm bacteria challenged with monochloramine retained significant respiratory activity, even though they could not be cultured on agar plates. Microbial colony counts on agar media declined by approximately 99.9% after 1 h of disinfection, whereas the number of bacteria stained by a fluorescent redox dye experienced a 93% reduction. Integrated measures of biofilm respiratory activity, including net oxygen and glucose utilization rates, showed only a 10 to 15% reduction. In this biofilm system, measures of microbial respiratory activity and

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Effects of various metal substrata on accumulation ofPseudomonas aeruginosabiofilms and the efficacy of monochloramine as a biocide

Cited by 21 publications

References 15 publications

The use of biocides to control sulphate‐reducing bacteria in biofilms on mild steel surfaces

The use of biocides to control sulphate‐reducing bacteria in biofilms on mild steel surfaces

Antimicrobial Tolerance in Biofilms

Comparison of respiratory activity and culturability during monochloramine disinfection of binary population biofilms

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