Effects of Various Growth Regulators on in Vitro Rooting of Globe Artichoke (Cynara scolymus L.)

Abstract

Since micropropagation is useful tool to get healthy initial material, main target of many researches on this subject is to develop and/or optimize an efficient protocol for artichoke cultivars. In this study, influences of different doses of growth regulators indole-3-butyric acid (IBA), naphthalene acetic acid (NAA), gibberellin (GA 3 ) in combination with/without activated charcoal (AC), putrescine acid and jasmonic acid, added to Murashige and Skoog (MS) basal medium have been investigated on the rooting of globe artichoke (Cynara scolymus L.). Artichoke offshoots from 4-year-old mother plants of globe artichoke cultivars Sakiz and Bayrampaşa were used. Results showed that the highest rooting percentage 63.35% and 36.6% was obtained in Sakiz and Bayrampaşa, respectively, from the 5 mg/L GA 3 with 1 g/L AC added to MS medium.

Comparative study on in vitro micropropagation response of seven globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] cultivars: open-pollinated cultivars vs F1 hybrids

“…Similar findings were also reported by Cavallaro et al (2004); Iapichino (2013);López-Pérez and Martínez (2015). As stated in previous studies, the most challenging part of in vitro micropropagation studies for globe artichoke is the in vitro rooting stage (López-Pérez and Martínez, 2015;Ercan, 2016). As seen in present study, in vitro rooting rate is quite low due to genotype-related differences.…”

“…To serve the purpose, indole-3-acetic acid (IAA, 10.0 mg L -1 ), indole butyric acid (IBA, 6.0 mg L -1 ), gibberellic acid (GA3, 5.0 mg L -1 ), and naphthalene acetic acid (NAA, 0.5 mg L -1 ) were used at different concentrations and combinations with ½ basic MS medium (Ozsan and Onus, 2019). The concentrations of plant growth regulators and activated charcoal (AC) doses (1.0 g L -1 and 2.0 g L -1 ) used in present study were based on previous studies (Ancora et al, 1981;Morzadec and Hourmant, 1997;Cavallaro et al, 2004;Tavazza et al, 2004;López-Pérez and Martínez, 2015;Ercan, 2016).…”