A protocol on in vitro rooting of ‘Bayrampaşa’ artichoke (Cynara scolymus L.)

Abstract

Artichoke, belongs to the family Asteraceae (Compositae), is cultivated in a very wide area in the world and regarded as a functional food due to the bioactive components. The propagation of artichoke via tissue culture offers considerable advantages. However one of the most important problem is in vitro rooting. Therefore, the main purpose of the present study was to develop an effective protocol on in vitro rooting of 'Bayrampaşa' cultivar which is one of the important local artichoke cultivar. To serve the purpose, after a successful micropropagation process, involving 3 subculture stages, well-developed plantlets were selected and rooted in different media compositions as ten different media including control group were used in present study. The differences between the media were provided by adding different growth regulators such as 10.0 mg l-1 indole-3-acetic acid (IAA), 6.0 mg l-1 indole butyric acid (IBA), 5.0 mg l-1 gibberellic acid (GA3), 0.5 mg l-1 naphthaleneacetic acid (NAA) and activated charcoal (0, 1.0 and 2.0 g l-1). Developments of plantlets were observed and recorded at 15 days intervals. According to the results obtained during the study, the medium containing IAA (10.0 mg l-1) and 1.0 g l-1 of activated charcoal gave the best results in terms of rooting after micropropagation.

Comparative study on in vitro micropropagation response of seven globe artichoke [Cynara cardunculus var. scolymus (L.) Fiori] cultivars: open-pollinated cultivars vs F1 hybrids

“…For root induction, various media combinations were assessed. To serve the purpose, indole-3-acetic acid (IAA, 10.0 mg L -1 ), indole butyric acid (IBA, 6.0 mg L -1 ), gibberellic acid (GA3, 5.0 mg L -1 ), and naphthalene acetic acid (NAA, 0.5 mg L -1 ) were used at different concentrations and combinations with ½ basic MS medium (Ozsan and Onus, 2019). The concentrations of plant growth regulators and activated charcoal (AC) doses (1.0 g L -1 and 2.0 g L -1 ) used in present study were based on previous studies (Ancora et al, 1981;Morzadec and Hourmant, 1997;Cavallaro et al, 2004;Tavazza et al, 2004;López-Pérez and Martínez, 2015;Ercan, 2016).…”