Effects of Various Drugs on Alcohol-induced Oxidative Stress in the Liver

Popović, Mira; Janićijević-Hudomal, Snežana; Kaurinović, Biljana; Rašić, Julijana; Trivić, Svetlana

doi:10.3390/molecules13092249

Cited by 17 publications

(12 citation statements)

References 28 publications

(29 reference statements)

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“…However, SOD and CAT activities were increased by CCl 4 , in contrast to GSP and GR. Several studies have found a decreased antioxidant enzymes expression following CCl 4 administration [57,58], others, including the current study, found increased levels after CCl 4 challenge [59][60][61]. One probable cause of this increase was suggested to be the high free radical challenge that the enzymes encounter, leading to their overexpression [62].…”

Section: Discussionsupporting

confidence: 47%

In-depth hepatoprotective mechanistic study of Phyllanthus niruri: In vitro and in vivo studies and its chemical characterization

et al. 2020

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Phyllanthus niruri L. is a widespread tropical plant which is used in Ayurvedic system for liver and kidney ailments. The present study aims at specifying the most active hepatoprotective extract of P. niruri and applying a bio-guided protocol to identify the active compounds responsible for this effect. P. niruri aerial parts were extracted separately with water, 50%, 70% and 80% ethanol. The cytoprotective activity of the extracts was evaluated against CCl 4 -induced hepatotoxicity in clone-9 and Hepg2 cells. Bioassay-guided fractionation of the aqueous extract (AE) was accomplished for the isolation of the active compounds. Antioxidant activity was assessed using DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging method and ferric reducing antioxidant power (FRAP). The in vivo hepatoprotective activity of AE was evaluated in CCl 4 -induced hepatotoxicity in rats at different doses after determination of its LD 50 . Pretreatment of clone-9 and Hepg2 with different concentrations of AE (1, 0.1, 0.01 mg/ml) had significantly reduced the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) against CCl 4 injures, and restored the activity of the natural antioxidants; glutathione (GSH) and superoxide dismutase (SOD) towards normalization. Fractionation of AE gave four fractions (I-IV). Fractions I, II, and IV showed a significant in vitro hepatoprotective activity. Purification of I, II and IV yielded seven compounds; corilagin C1, isocorilagin C2, brevifolin C3, quercetin C4, kaempferol rhamnoside C5, gallic acid C6, and brevifolin carboxylic acid C7. Compounds C1, C2, C5, and C7 showed the highest (p< 0.001) hepatoprotective potency, while C3, C4, and C6 exhibited a moderate (p< 0.001) activity. The AE exhibited strong antioxidant DPPH (IC 50 11.6 ± 2 μg/ml) and FRAP (79.352 ± 2.88 mM Ferrous equivalents) activity. In vivo administration of AE in rats (25, 50, 100 and 200 mg/kg) caused normalization of AST, ALT, alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total cholesterol (TC), triglycyrides (TG), total bilirubin (TB), glucose, total proteins (TP), urea and creatinine levels which were elevated by CCl 4 . AE also decreased TNF-α, NF-KB, IL-6, IL-8, IL10 and COX-2 expression, and significantly antagonizes the effect of CCl 4 on the antioxidant enzymes SOD, catalase PLOS ONE | https://doi.org/10.1371/journal.pone.0226185 January 15, 2020 1 / 23 OPEN ACCESS Citation: Ezzat MI, Okba MM, Ahmed SH, El-Banna HA, Prince A, Mohamed SO, et al. (2020) In-depth hepatoprotective mechanistic study of Phyllanthus niruri: In vitro and in vivo studies and its chemical characterization. PLoS ONE 15(1): e0226185.

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Section: Discussionsupporting

confidence: 47%

In-depth hepatoprotective mechanistic study of Phyllanthus niruri: In vitro and in vivo studies and its chemical characterization

et al. 2020

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“…Hepatic toxicity of the antipsychotic HP has been reported in cultured rat hepatocytes 26 and experimentally in Wistar rats. 27,28 Shivakumar et al (1992) reported that HP blockade of dopamine receptor increases dopamine turnover resulting in excessive production of hydrogen peroxide and, thus, generates oxidative stress. 17 Haloperidol-induced oxidative stress was associated with an increased generation of brain NO which is a free radical which can be deleterious to brain functions, where synthesis of inducible nitric oxide synthase contributes to the activation of apoptotic pathways in the brain.…”

Section: Discussionmentioning

confidence: 99%

Alleviation of haloperidol induced oxidative stress in rats: Effects of sucrose vs grape seed extract

Awdan

Jaleel

Saleh

2015

Bulletin of Faculty of Pharmacy, Cairo University

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Haloperidol (HP) is a classic antipsychotic drug known for its propensity to cause extrapyramidal side effects. HP is known to induce oxidative stress due to increased turnover of dopamine. The aim of the present study was to investigate the effect of sucrose (1 and 5 mg/kg; p.o.) and grape seed extract (GSE; 100, 200 and 400 mg/kg; p.o.) on the oxidative stress induced in rats by HP (1 mg/kg; p.o.) in the liver and the brain tissues. Oxidative stress was induced by injection of HP for 14 consecutive days which was concurrently administered with sucrose and GSE. Liver and brain levels of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (nitrite) levels were determined in the brain and liver. Results of the present study revealed that HP-treated rats showed elevated levels of NO in the brain and MDA in the brain and liver. HP-treated rats showed also decreased levels of NO levels in the liver and GSH in the brain and liver. Treatment of HP-treated rats with GSE reversed all the oxidative stress markers in both the brain and liver due to its potent antioxidant property. On the other hand, sucrose attenuates the levels of NO in the brain and liver and the brain levels of MDA and GSH. It can be concluded that both GSE (a potent anti-oxidant) and sucrose (as a source of energy) have beneficial impacts on the brains of HP-treated rats. However, GSE is more potent in alleviating the oxidative stress associated with HP in the liver. ª 2015 Production and hosting by Elsevier B.V. on behalf

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“…Alcohol is metabolized by three pathways which utilize alcohol dehydrogenase, the microsomal ethanol oxidative system (MEOS) and catalase, respectively [35]. MEOS, characterized by CYP2E1, is viewed as the main site of ROS overproduction during alcohol metabolism.…”

Section: Discussionmentioning

confidence: 99%

Effects of Triterpenoid From Schisandra chinensis on Oxidative Stress in Alcohol-Induced Liver Injury in Rats

Zhu

et al. 2014

Cell Biochem Biophys

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Alcohol-induced oxidative stress plays a crucial role in the pathological development of alcoholic liver disease. The aim of this study was to investigate the effects of triterpenoid from Schisandra chinensis on oxidative stress in alcohol-induced liver injury in rats. We found that the administration of triterpenoid attenuated alcohol-induced oxidative stress in multiple organs including liver. Moreover, the impaired liver function and histological changes resulted from alcohol consumption was improved by triterpenoid treatment. Finally, we found that pretreatment with triterpenoid from Schisandra chinensis to alcohol-fed rats increased the expression level of haem oxygenase-1 (HO-1) while inhibited the induction of cytochrome P-450 2E1 (CYP2E1) in liver microsomes. Further assays revealed that the microsomal activity of HO-1 was accordingly induced whereas CYP2E1 was suppressed in rats received triterpenoid intervention. Our findings suggest that triterpenoid from Schisandra chinensis may protect against alcohol-induced liver injury through ameliorating oxidative stress in rats.

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Effects of Various Drugs on Alcohol-induced Oxidative Stress in the Liver

Cited by 17 publications

References 28 publications

In-depth hepatoprotective mechanistic study of Phyllanthus niruri: In vitro and in vivo studies and its chemical characterization

In-depth hepatoprotective mechanistic study of Phyllanthus niruri: In vitro and in vivo studies and its chemical characterization

Alleviation of haloperidol induced oxidative stress in rats: Effects of sucrose vs grape seed extract

Effects of Triterpenoid From Schisandra chinensis on Oxidative Stress in Alcohol-Induced Liver Injury in Rats

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