Effects of Various Additives on in Vitro Parameters Related to Viability and Function of Stored Canine Blood*

Eisenbrandt, David L.; Smith, Joseph

doi:10.1016/s0034-5288(18)33688-9

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…Stored cells that do not survive 24 hours in a recipient are considered nonviable. Current Food and Drug Administration (FDA) requirements for stored human whole blood or red blood cells state that at least 75% of the transfused cells must remain viable 24 hours after transfusion if a preservative is to be considered satis-factoryB The in vivo evaluation of viability of stored red blood cells is accomplished by radiolabeling the cells, generally with 5'Cr, infusing them into a recipient, and following their fate over time in the circulation. "…”

Section: Introductionmentioning

confidence: 99%

An in vitro Evaluation of Storage Media for the Preservation of Canine Packed Red Blood Cells

Wardrop

Young

Wilson

1994

Veterinary Clinical Pathol

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The effect of four different red blood cell storage media on in vitro parameters of stored canine red blood cells was studied. The storage media included citrate-phosphate-dextrose-adenine (CPDA-1), two additive solutions, and an additive solution modified by the addition of plasma. Biochemical and hematologic parameters, including red cell adenosine triphosphate (ATP); 2,3-diphosphoglycerate (2,3-DPG); pH; percent hemolysis; and supernatant sodium, potassium, and glucose were assessed immediately following preparation of the red cell concentrate and after 35 and 42 days of storage at 4 degrees C. All parameters changed significantly (p < 0.05) during storage. Significant differences due to effect of the storage media were also seen at each time period. After 35 days and 42 days of storage, CPDA-1 maintained the highest pH, potassium, and sodium values, and had the lowest 2,3-DPG, ATP (p=0.052), and glucose values. No differences were seen in hemolysis after 35 days of storage. No additional benefit was noted from the addition of plasma to the additive solution. The additive solutions compared favorably with CPDA-1.

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Section: Introductionmentioning

confidence: 99%

An in vitro Evaluation of Storage Media for the Preservation of Canine Packed Red Blood Cells

Wardrop

Young

Wilson

1994

Veterinary Clinical Pathol

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“…In 1 study, 10 2 of 6 units of blood were modified with a solution of ascorbic acid, which changed the Hct of these 2 units but not that of the untreated (control) units. In another study, 11 pH of the control units used for comparison varied, which may have confounded results of that study. Differences in both Hct and pH can, individually, have a significant effect on the outcome of RBC storage.…”

Section: Discussionmentioning

confidence: 95%

“…[61][62][63][64][65][66][67] On day 7, the 2,3-DPG concentration of stored RBCs in the present study was higher than the day 0 values reported in some studies [2][3][4] but similar to initial values reported in other studies. 10,11,68 Previous studies 10,11 on the storage of canine RBCs have involved the use of ascorbic acid to supplement additive solutions and have found no beneficial effect. Although ascorbic acid concentrations similar to those in other studies were used in the present study, sample sizes were small, and RBC storage methods differed.…”

Section: Discussionmentioning

confidence: 99%

“…for up to 42 days in additive solutions. 1,9 Canine fresh whole blood has been stored in solutions consisting of acid-citrate-dextrose [10][11][12][13] or CPD 11,12 with or without chemical additives 10,11 as well as in a storage medium for canine blood. 13 Canine RBCs have also been stored in CPDA-1, [2][3][4] licensed RBC additive solutions (AS-1 2,3,14 and AS-3 3,4 ), and AS-3 supplemented with other factors.…”

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confidence: 99%

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Effect of ascorbic acid on storage of Greyhound erythrocytes

Fontes¹,

Banerjee²,

Iazbik³

et al. 2015

ajvr

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Evaluation of Citrate‐Phosphate‐ Dextrose‐Adenine as a Storage Medium for Packed Canine Erythrocytes

Price

Armstrong²,

McLeod

et al. 1988

Veterinary Internal Medicne

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Packed canine red blood cells (RBCs) stored in the anticoagulant-preservative solution citrate-phosphate-dextrose-adenine (CPDA-1) were studied at 1,10, 20,30, and 40 days. The extracellular concentrations of potassium and sodium, erythrocyte mean corpuscular volume, and osmotic fragility increased during storage (P < 0.05). There was a decrease in the pH, plasma concentration of g!ucose, and erythrocyte concentrations of 2,3-diphosphoglycerate (2,3-DPG) and adenosine-5'-triphosphate (P < 0.05). Erythrocyte 2,3-DPG concentration decreased by 54% within the first 24 hours of storage (P < 0.001). Posttransfusion viability (PTV) decreased from !M% on day 1 to 46% on day 40 (P < 0.05).The PTV of the RBCs stored for 10 and 20 days complied with the Food and Drug Administration (FDA) standard.Although there are marked biochemical and hematologic changes in stored packed red blood cells (pRBCs), 20-day-old units may be expected to be of acceptable quality. The sharp decrease in 2,3-DPG concentration suggests a reduction in oxygen carrying capacity in erythrocytes stored as pRBCs. Hyperkalemia occurs during storage of pRBCs and does not appear to be associated with high intraerythrocytic potassium concentrations. (Journal of Veterinary Internal Medicine 1988; 2:126-132) THE USE OF blood components rather than whole blood for replacement therapy has become a common practice in canine transfusion medicine. Separation of a unit of whole blood into packed red blood cells (pRBCs) and fresh-frozen or platelet-rich plasma facilitates storage and increases the utility of donated blood. Specific component hemotherapy avoids hypervolemia in anemic, normoproteinemic patients and polycythemia in nonanemic patients with coagulation factor deficiencies, hypoalbuminernia, or thrombocytopenia.The methodology currently recommended to prepare and store canine blood components has largely been extrapolated from human transfusion medicine tech-

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Effects of Various Additives on in Vitro Parameters Related to Viability and Function of Stored Canine Blood*

Cited by 4 publications

References 25 publications

An in vitro Evaluation of Storage Media for the Preservation of Canine Packed Red Blood Cells

An in vitro Evaluation of Storage Media for the Preservation of Canine Packed Red Blood Cells

Effect of ascorbic acid on storage of Greyhound erythrocytes

Evaluation of Citrate‐Phosphate‐ Dextrose‐Adenine as a Storage Medium for Packed Canine Erythrocytes

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