Effects of vanadate on cardiac contraction and adenylate cyclase

Grupp, Günter; Grupp, I L; Johnson, Carl L.; Wallick, Earl T.; Schwartz, Arnold

doi:10.1016/0006-291x(79)92068-0

Cited by 74 publications

(21 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2) Increasing cellular cAMP helps to induce a mitogenic response in a variety of different cultured animal cells (10,11,50,51). Moreover, vanadate has been reported to activate adenylate cyclase (52)(53)(54) and increase cAMP levels in isolated tissues (55,56 (1983) than synergistic effects on glucose transport and oxidation (43). The marked synergism between insulin and vanadium that we observed for the stimulation of DNA synthesis suggests that vanadium is not acting solely in an insulin-like fashion in the 3T3 and 3T6 cells.…”

Section: Discussionmentioning

confidence: 99%

Vanadium ions stimulate DNA synthesis in Swiss mouse 3T3 and 3T6 cells.

Smith¹

1983

Proc. Natl. Acad. Sci. U.S.A.

126

View full text Add to dashboard Cite

Vanadyl sulfate and sodium orthovanadate in the concentration range between 5 and 50 IAM are shown to be mitogenic for quiescent cultures of Swiss mouse 3T3 and 3T6 cells. The compounds caused a striking shift in the dose-response for the effect of serum on [3Hjthymidine incorporation and DNA synthesis. In the absence of serum the effect of vanadium was greatly potentiated by insulin. Vanadium ionr produced no more than additive increases in [3H]thymidine. incorporation when combined with epidermal growth factor, cholera toxin, or phorbol 12-myristate 13-acetate. Both vanadium compounds stimulated ouabaininhibitable 86Rb+ uptake, indicating that the vanadium ions increase, rather than inhibit, Na+/K' pump activity in the intact cell. Neither vanadium compound had any effect on cellular cAMP under a variety of different conditions. The mitogenic effect of the vanadium compounds was similar to that of colehicine. Taxol, which stabilizes cytoplasmic microtubules, prevented the stimulation of DNA synthesis by vanadium.Quiescent cultures of mammalian cells can be induced to synthesize DNA and proliferate by certain combinations of growthstimulating compounds (1-3). Several animal cell lines have been continuously passaged for many generations in a serum-free medium supplemented with an optimal combination of growthpromoting hormones (2). In fibroblastic Swiss mouse 3T3 cells; a variety of different agents have been identified as potent comitogens: epidermal growth factor (EGF) (4); insulin-like growth factors (5); vasopressin-(6); the tumor promoter phorbol 12-myristate 13-acetate (PMA) (7, 8); platelet-derived growth factor (PDGF) (9); cholera toxin (10, 11); sarcoma-derived growth factor (12); and antitubulin agents (13)(14)(15)(16).Some of these agents alter distinctly different cellular processes, whereas others appear to have a common mechanism of action. For example, cholera toxin increases cAMP (11), and EGF and PDGF modulate phosphotyrosine formation via a protein kinase (17,18). Studies with colchicine and other antitubulin compounds suggest that the depolymerization of cytoplasmic microtubules enhances the mitogenic response to various growth factors (13)(14)(15)(16). Vasopressin and PMA seem to share a common mechanism (19), perhaps involving an increase in the permeability of the cell to ions (20,21). A greater permeability to Na ions appears to be at least partially responsible for the increase in Na+/K' pump activity (22), which occurs shortly after mitogen addition in a variety of different cell types (23-28).We are using vanadium compounds to help in the identification of specific biochemical events involved in the stimulation of DNA synthesis and proliferation in quiescent cells in vitro. * Vanadium is an essential nutrient for higher animals (30), but no specific physiologic role of vanadium has been identified. In the rat, a dietary deficiency of vanadium produces a general retardation of growth, and only about 100 parts perk billion (2 nM) vanadium is needed for normal growth (30). McKeeh...

show abstract

Section: Discussionmentioning

confidence: 99%

Vanadium ions stimulate DNA synthesis in Swiss mouse 3T3 and 3T6 cells.

Smith¹

1983

Proc. Natl. Acad. Sci. U.S.A.

126

View full text Add to dashboard Cite

show abstract

“…In certain heart muscles, inhibitions of Na, K-ATPase is thought to be main cause regarding positive inotropic effects of venadate (Hackbarth et al, 1978;Grupp et al, 1979;Bochard et al, 1979;Myers & Boerth, 1980) similar to the action of ouabain, while, in other heart muscles, vanadate shows a negative inotropic effect (Akeraet al, 1979;Grupp et al, 1979;Solaro et al, 1980). Vanadate has also an inhibitory action on CaATPase activity and Ca transport of sarcolmma and sarcoplasmic reticulum of heart muscle (Wang et al, 1979;Solaro et al, 1980;Wierichs et al, 1980;Caroni & Carafori, 1981a;Caroni & Carafori, 1981b;Morcos, 1982) and sarcoplasmic reticulum of skeletal muscle (O'Neal et al, 1979;Wang et al, 1979).…”

Section: Researchmentioning

confidence: 99%

Effects of soldium vanadate on the smooth muscle of the guinea-pig vas deferens.

Sunano

Shimada

Shimamura

1985

Jpn. J. Smooth Muscle Res.

View full text Add to dashboard Cite

show abstract

“…Vanadate has been found to inhibit (Na, K)-ATrase (Cantley et al, 1977(Cantley et al, , 1978Nechay and Saunders, 1978;Goodno, 1979;Nieder et al, 1979) and activate cardiac adenylate cyclase (GRUPP et al, 1979). Signs of toxicosis in calves and lambs include diarrhea, depressed growth and performance, ataxia, and mortality.…”

Section: Discussionmentioning

confidence: 99%

Vanadium concentration in mineral salt mixtures used as supplementation in bovines

Marçal

Watanabe

2016

Revista Brasileira De Higiene E Sanidade Animal

View full text Add to dashboard Cite

Resumo: O comércio de suplementos minerais para bovinos no Brasil cresceu muito desde a última década. Muito embora seja necessária a utilização de matérias primas sem contaminantes, é possivel que alguns fabricantes ainda utilizem fontes de fósforo mais baratas. Essa situação pode veicular metais pesados na mistura mineral pronta, o que, em médio prazo, pode intoxicar os animais. Por essa razão, foi mensurada a concentração de Vanádio nas amostras de misturas de sal mineral usadas na suplementação de gado de corte nos estados do Paraná, Brasil. A concentração de vanádio foi determinada pela indução da espectrometria acoplada à emissão de plasma atômico. Das 22 amostras analisadas, 17 tinham valores maiores que 50 ppm (variando entre 66 e 128 ppm), a concentração máxima recomendada pelo Conselho Nacional de Pesquisa e pela Associação Americana de Controle Oficial de Alimentação. Esses resultados mostram a necessidade de um monitoramento industrial cuidadoso, porque algumas misturas de minerais contém quantidade suficiente de vanádio para causar intoxicação nos animais. Termos para indexação: animais, intoxicação, misturas de minerais, monitoramento industrial.Abstract: The commercialization of mineral supplements for cattle in Brazil has grown a lot since the last decade. Although it is necessary to use raw materials without contaminants, it is possible that some manufacturers still use cheaper sources of phosphorus. This situation can serve heavy metals in the ready mineral mixture, which in the medium term, can poison animals. For this reason, we measured vanadium concentrations in samples of mineral mixtures used in cattle feed. Vanadium concentration was determined by inductively coupled plasma atomic emission spectrometry. Of the 22 analyzed samples, 17 had values greater than 50 ppm (ranging between 66 to 128 ppm), which is the maximum concentration recommended by the National Research Council and the Association of American Feed Control Officials Incorporated. These findings show the necessity for careful industrial monitoring because some mineral mixtures contain sufficient vanadium to cause toxicity in animals.

show abstract

Effects of vanadate on cardiac contraction and adenylate cyclase

Cited by 74 publications

References 9 publications

Vanadium ions stimulate DNA synthesis in Swiss mouse 3T3 and 3T6 cells.

Vanadium ions stimulate DNA synthesis in Swiss mouse 3T3 and 3T6 cells.

Effects of soldium vanadate on the smooth muscle of the guinea-pig vas deferens.

Vanadium concentration in mineral salt mixtures used as supplementation in bovines

Contact Info

Product

Resources

About