Effects of Urine on Motility and Fertilizing Capacity of Rabbit Spermatozoa

Chang, M. C.; Þorsteinsson, Þorsteinn

doi:10.1016/s0015-0282(16)33065-5

Cited by 10 publications

(2 citation statements)

References 6 publications

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“…It seems reasonable to incriminate the lowered spermatozoan motility as the factor responsible for the reduced ability of sperma¬ tozoa irradiated with > 10,000 r to compete with their untreated counterparts, since this, and much higher dose levels of irradiation, apparently do not affect the organdíes of the sperm head which facilitate its entry into the egg. These experiments, therefore, may also provide some direct experimental evidence for the well-established view that quality of sperm motility is one important index of semen fertility, although it must be remembered that poor sperm motility is not necessarily incompatible with fertility (Chang & Thorsteinsson, 1958).…”

Section: Discussionmentioning

confidence: 92%

The Influence of X-Irradiation of Rabbit Spermatozoa on Fertilization and Early Cleavage

Bedford¹,

Hunter²

1968

Reproduction

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Summary. Exposure of rabbit semen to X-irradiation did not reduce the fertilization rate until doses of >25,000 r were used, although motility was noticeably affected at 15,000 r. Evidence of a depressant effect of much lower doses of X-irradiation on the fertilizing ability of rabbit spermatozoa was obtained, however, by using a more sensitive system in which irradiated aliquots from an ejaculate were inseminated mixed together with an exactly equivalent volume of untreated semen from the same ejaculate. Under these competitive conditions the fertilizing ability of spermatozoa was found not to be affected by a dose of 10,000 r, but was significantly depressed (P<0\m=.\01)by 15,000 r, as judged by an increase in the proportion of normally developing to retarded ova, recovered 50 hr after an ovulation injection of hcg. It appears that this depressant effect is exerted at the site of fertilization rather than on sperm transport. X-irradiation of spermatozoa did not increase polyspermic or delayed fertilization, neither did irradiation have any significant effect on the formation of the male pronucleus. A dose response effect was seen at the time of the first cleavage division, for at 24 hr the cleavage rate of control ova (68\m=.\5%) was similar to that of ova fertilized by spermatozoa treated with 10,000 r (68%), but only 33% of ova fertilized by spermatozoa treated with 20,000 to 25,000 r had cleaved at this time compared with 78% of their controls. At 29 and 50 hr several ova fertilized by irradiated spermatozoa remained at the pronuclear stage or at metaphase of the first cleavage division, and even at 50 hr the great majority had only reached the 2-cell stage. Thus, although it is known that cleavage can occur in the absence of the male elements, the first cleavage division does not appear to follow activation of the egg automatically and independently since it has been shown that the state of the male elements introduced at fertilization can influence the rate and ultimate success of the changes which take place at this time.

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Section: Discussionmentioning

confidence: 92%

The Influence of X-Irradiation of Rabbit Spermatozoa on Fertilization and Early Cleavage

Bedford¹,

Hunter²

1968

Reproduction

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“…Fertilization has been reported from the insemination of nonmotile spermatozoa (78,80) ; however, this is not to say that the sperm cells did not become motile upon exposure to the female tract fluids. That there is a stimulation in metabolic rate and motility in the vicinity of the egg is indicated by the results of several studies.…”

Section: Motility In the Female Reproductive Tractmentioning

confidence: 99%

Metabolism of Spermatozoa

Salisbury¹,

Lodge²

1962

Advances in Enzymology - And Related Areas of Molecular Biology

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Vasocystostomy in the Rabbit: Surgical Technique and Sperm Outflow

Hooker¹

1981

Journal of Andrology

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The surgical technique employed for construction of bilateral vas‐to‐bladder fistulae (vasocystostomy) in the male rabbit Is described. The method of collection and results of examination of sperm from 15 of these animals are presented. The average daily number of intact sperm (185 $4 106) passed in the urine of vasocystostomized rabbits was consistent with previous estimates of sperm production and slightly exceeded previous measurements of sperm output in ejaculated semen.

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Effects of Urine on Motility and Fertilizing Capacity of Rabbit Spermatozoa

Cited by 10 publications

References 6 publications

The Influence of X-Irradiation of Rabbit Spermatozoa on Fertilization and Early Cleavage

The Influence of X-Irradiation of Rabbit Spermatozoa on Fertilization and Early Cleavage

Metabolism of Spermatozoa

Vasocystostomy in the Rabbit: Surgical Technique and Sperm Outflow

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