Effects of urethane on hippocampal unit activity in the rat

Mercer, L.F.; Remley, N.R.; Gilman, Dennis P.

doi:10.1016/0361-9230(78)90089-8

Cited by 45 publications

(9 citation statements)

References 8 publications

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“…8A). Bath application of urethane at 10 mM, matching the drug concentration achieved in vivo at doses of 0.5-1.0 g/kg (Sceniak and MacIver, 2006) used in the present study, reduced the baseline activity to 0.01 Ϯ 0.01 s Ϫ1 (n ϭ 21 cells; P2-P5; p ϭ 5.7 ⅐ 10 Ϫ14 ; Mann-Whitney test), which is in keeping with a depressant action of urethane on neuronal firing (Mercer et al, 1978;Sceniak and MacIver, 2006). However, GABA application in the presence of urethane still increased neuronal firing, reaching a frequency of 23.66 Ϯ 4.97 s Ϫ1 during responses (n ϭ 21 cells; P2-P5; p ϭ 4.8 ⅐ 10…”

Section: Dependence Of Excitatory Action Of Gaba In Neonatal Slices Osupporting

confidence: 87%

An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons

et al. 2016

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To investigate excitatory and inhibitory GABA actions in cortical neuronal networks, we present a novel optogenetic approach using a mouse knock-in line with conditional expression of channelrhodopsin-2 (ChR2) in GABAergic interneurons. During whole-cell recordings from hippocampal and neocortical slices from postnatal day (P) 2-P15 mice, photostimulation caused depolarization and excitation of interneurons and evoked barrages of postsynaptic GABAergic currents. Excitatory/inhibitory GABA actions on pyramidal cells were assessed by monitoring the alteration in the frequency of EPSCs during photostimulation of interneurons. We found that in slices from P2-P8 mice, photostimulation evoked an increase in EPSC frequency, whereas in P9 -P15 mice the response switched to a reduction in EPSC frequency, indicating a developmental excitatory-to-inhibitory switch in GABA actions on glutamatergic neurons. Using a similar approach in urethane-anesthetized animals in vivo, we found that photostimulation of interneurons reduces EPSC frequency at ages P3-P9. Thus, expression of ChR2 in GABAergic interneurons of mice enables selective photostimulation of interneurons during the early postnatal period, and these mice display a developmental excitatory-to-inhibitory switch in GABA action in cortical slices in vitro, but so far show mainly inhibitory GABA actions on spontaneous EPSCs in the immature hippocampus and neocortex in vivo.

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Section: Dependence Of Excitatory Action Of Gaba In Neonatal Slices Osupporting

confidence: 87%

An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons

et al. 2016

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“…GILBERT AND MACK tal have also been reported to decrease PS amplitude, increase the threshold for PS elicitation (Bennett et al, 1991;Engstrom et al, 1990), and reduce unit firing in hippocampal neurons (Mercer et al, 1978). In the present study, an anesthesia-induced suppression of evoked activity was also observed as higher intensity stimulation was required to produce submaximal PS amplitudes in the acutely anesthetized preparation relative to the chronically implanted animals administered urethane.…”

supporting

confidence: 62%

Field potential recordings in dentate gyrus of anesthetized rats: Stability of baseline

Gilbert

Mack

1999

Hippocampus

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“…Sixteenchannel linear electrode array recordings of LFPs across the somatodendritic axis of dorsal hippocampus G) under urethane anesthesia and H) in the freely-behaving mouse. Under urethane, LFPs reflecting rhythmic ongoing synaptic activity is much attenuated compared to in the behaving mouse 42,43 . Voltage scale (red bar) = 1 mV.…”

Section: A Neocortical-hippocampal Subcircuit Tuned By Cognitive Traimentioning

confidence: 92%

Learning to learn persistently modifies an entorhinal-hippocampal excitatory-inhibitory subcircuit

Chung

Jou

Grau‐Perales

et al. 2019

Preprint

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SUMMARYThe neurobiology of psychological concepts like schema, and psychotherapeutic strategies of cognitive behavioral therapy (CBT) is poorly understood, partly because learning to process information confounds, and is rarely distinguished from acquiring content-specific memory. Learning to learn changes one’s overall information-processing ability, whereas neurobiological investigations typically focus on memory for content from particular experiences. We investigated entorhinal cortex-to-dentate gyrus neural circuit changes while mice learn to learn during cognitive control training (CCT) to judiciously use and ignore information. CCT changes synaptic circuit function by persistently modifying an excitatory-inhibitory interneuron subcircuit lasting weeks. CCT increases dentate gyrus expression of PKMζ that maintains long-term potentiation, particularly in somatostatin-expressing inhibitory interneurons that mediate both widespread inhibition, and through disinhibition, also local excitation of dentate gyrus. These findings that CCT modifies excitation-inhibition circuit coordination provide direct neurobiological evidence for a CBT-neuroplasticity hypothesis that, beyond particular item/event associations, learning to learn persistently changes neural circuit function.

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Effects of urethane on hippocampal unit activity in the rat

Cited by 45 publications

References 8 publications

An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons

An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons

Field potential recordings in dentate gyrus of anesthetized rats: Stability of baseline

Learning to learn persistently modifies an entorhinal-hippocampal excitatory-inhibitory subcircuit

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