Effects of unilateral nasal obstruction on the characteristics of jaw-closing muscles in growing rats

Tang, Huan; Yonemitsu, Ikuo; Ikeda, Yuhei; Watakabe, Kenzo; Shibata, Shunichi; Hosomichi, Jun; Ono, Takashi

doi:10.2319/021918-132.1

Cited by 8 publications

(7 citation statements)

References 30 publications

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“…These results indicate that hypoxia can lead to oxidative stress and apoptosis in periodontal tissue. After modeling UNO in rats, rats presented with oral respiratory symptoms [24][25][26]. Oral respiration is one of the main clinical manifestations in patients with OSAHS [1].…”

Section: Discussionmentioning

confidence: 99%

“…Model. A UNO rat model was established by cauterizing the external nostril [24][25][26]. Briefly, 8-day-old male Wistar rats (n = 30) were randomly divided into two groups: SHAM and UNO.…”

Section: Animals and Unilateral Nasal Obstruction (Uno)mentioning

confidence: 99%

“…Tissue in UNO Rats. The establishment of the UNO rat model was as mentioned above [24][25][26]. We used the peripheral nostril obstruction in rats as the standard for successful modeling.…”

Section: Systemic Changes and Increased Apoptosis Of Periodontalmentioning

confidence: 99%

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Effects of Hypoxic Environment on Periodontal Tissue through the ROS/TXNIP/NLRP3 Inflammasome Pathway

Zhu

2022

BioMed Research International

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There is low evidence for the possible association between obstructive sleep apnea-hypopnea syndrome (OSAHS) and periodontitis, necessitating further research. This study was aimed at investigating this association. For the in vitro study, 8-day-old Wistar rats were divided into the unilateral nasal obstruction group (UNO) and the sham surgery group (SHAM). Rats in the former group were subjected to UNO by cauterization of the external nostril at the age of 8 days. Immunofluorescence analysis, quantitative real-time polymerase chain reaction, and western blot were performed to assess the expression of thioredoxin-interacting protein (TXNIP), NLR family pyrin domain-containing 3 (NLRP3) inflammasome-associated factors, and interleukin-1β (IL-1β). Throughout the experimental period, the weights of rats in the two groups were similar. The mRNA and protein expression of TXNIP and IL-1β was significantly higher in the UNO than in the SHAM groups. Compared with SHAM, NLRP3 inflammasome-associated factors were activated in the UNO group. For the in vitro study, a cellular hypoxia model was established by treating human periodontal ligament cells (HPDLCs) with cobalt chloride. The studies showed that hypoxia can induce an excessive production and accumulation of reactive oxygen species (ROS) in HPDLCs and induce abnormal expression of TNXIP, NLRP3 inflammasome-related factors, and IL-1β. More importantly, N-acetylcysteine induced reduction of ROS in HPDLCs, downregulated TXNIP expression, inhibited the expression and aggregation of NLRP3 inflammasome-related factors, and abrogated the inflammatory response to hypoxia. In conclusion, hypoxia-induced ROS can activate the TXNIP/NLRP3 inflammasome signaling pathway in response to oxidative stress, resulting in the increased expression of inflammatory factors in HPDLCs. Our findings provide evidence for the mechanism underlying the possible association between OSAHS and periodontal disease.

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Section: Discussionmentioning

confidence: 99%

“…Model. A UNO rat model was established by cauterizing the external nostril [24][25][26]. Briefly, 8-day-old male Wistar rats (n = 30) were randomly divided into two groups: SHAM and UNO.…”

Section: Animals and Unilateral Nasal Obstruction (Uno)mentioning

confidence: 99%

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Effects of Hypoxic Environment on Periodontal Tissue through the ROS/TXNIP/NLRP3 Inflammasome Pathway

Zhu

2022

BioMed Research International

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“…The AD muscles of rats undergoing nasal obstructive treatment contain more myosin heavy chain (MHC)-II-X. As a result, the muscle generates a greater maximum speci c force, faster shorting velocity, and lower resistance to fatigue (Padzys et al, 2011;Tang et al, 2019). UNO may lead to respiratory muscle adaptation and produce motor modi cations associated with alterations in speci c EMG activity of the jaw-opening muscles.…”

Section: Reliability Of the Icms-de Ned Motor Mapsmentioning

confidence: 99%

“…Unilateral nasal obstruction (UNO) in a rat study induced changes in masticatory muscle activity, including reduced growth of the masseter muscle and anterior digastric (AD) muscles. As a result, the fatigue-resistant myosin heavy chain ber type was increased (Gelhaye et al, 2006;Tang et al, 2019). In addition, it has been demonstrated that during the growth period, UNO reduces the corticofugal effects of intracortical microstimulation (ICMS) in the primary motor cortex.…”

Section: Introductionmentioning

confidence: 99%

Effects of Unilateral Nasal Obstruction on the Development of the Cortical Masticatory Area in Growing Rats

Fujita¹,

Kato²,

Abe³

et al. 2021

Preprint

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The cortical masticatory area (CMA) regulates masticatory movements and controls rhythmic jaw movements. However, information regarding the effect of respiratory disturbances on the functional development of the CMA remains limited. This study aimed to examine the effect of chronic unilateral nasal obstruction (UNO) on the motor representation of the anterior digastric (AD) muscle during the growth period. Forty-eight 8-day-old male Wistar albino rats were randomized into control (CONT, n=24) and UNO (n=24) groups. Both groups underwent intracortical microstimulation (ICMS) at ages 5, 7, and 9 weeks. Arterial oxygen saturation (SpO2) and the number of positive ICMS sites for the AD muscle were measured throughout the experiment. The SpO2 values were significantly lower in the UNO group than in the CONT group at each age. Further, the number of positive ICMS sites for the AD muscle was significantly higher in the UNO group than in the CONT group at each age. Intragroup comparisons revealed that the number of positive ICMS sites increased with age. The onset latencies of the evoked AD potential significantly differed between the right and left sides. However, no differences were found between the CONT and UNO groups at any age. These findings suggest that UNO during development induces changes in the motor representation in the CMA.

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Cytokines, Masticatory Muscle Inflammation, and Pain: an Update

2020

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Effects of unilateral nasal obstruction on the characteristics of jaw-closing muscles in growing rats

Abstract: Unilateral nasal obstruction could affect the morphology and contractile characteristics of jaw-closing muscles during growth in rats.

Cited by 8 publications

References 30 publications

Effects of Hypoxic Environment on Periodontal Tissue through the ROS/TXNIP/NLRP3 Inflammasome Pathway

Effects of Hypoxic Environment on Periodontal Tissue through the ROS/TXNIP/NLRP3 Inflammasome Pathway

Effects of Unilateral Nasal Obstruction on the Development of the Cortical Masticatory Area in Growing Rats

Cytokines, Masticatory Muscle Inflammation, and Pain: an Update

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