Effects of unfractionated and fractionated heparins on myeloperoxidase activity and interactions with endothelial cells: Possible effects on the pathophysiology of equine laminitis

An update on equine laminitis

“…MARTINS FILHO et al (2008) found no reduction in hoof injuries with heparin treatment in the start of lameness, when laminitis was induced for carbohydrate administration. However, the substance prevented laminitis in horses with proximal duodenum jejunitis (LA REBIERE et al, 2008). Additionally to the antithrombotic effect of heparin, it is suggested that the substance contain anti-inflammatory effects.…”

What's new in laminitis research? II: advances in laminitis treatment

“…Heparin therapy does not prevent the development of clinical or histopathologic signs of laminitis in experimental models of CHO-induced laminitis [3,8]. However, in studies using equine digital endothelium, both unfractionated heparin and low-molecular-weight heparin (LMWH) effectively inhibited neutrophil-derived myeloperoxidase production [9], suggesting a potential application for heparin in the prevention of SIRS-associated laminitis. In a clinical study of horses treated for surgical colic, the rate and severity of postoperative laminitis were significantly lower in the horses treated with LMWH than in those not treated with heparin [10].…”

“…Another clinical study reported a significantly lower incidence of laminitis in horses with proximal enteritis treated with heparin than in those not treated with heparin [11]. The recommended dose of heparin often used is 100 IU/kg IV q8h [9]. Regenerative Therapies: Epidermal stem cells are found throughout the coronary and lamellar epithelia in the healthy foot.…”

Effect of endotoxin on leukocyte activation and migration into laminar tissue of isolated perfused equine limbs

“…6,8,10 Leukocyte activation, adhesion, and migration in several equine tissues have been assessed by the use of immunohistochemical detection of myeloperoxidase or calprotectin. [11][12][13][14][15][16][17] Myeloperoxidase, which can be regarded as a specific marker for the presence of activated neutrophils, 11,18 is an important enzyme used by neutrophils during phagocytic lysis of microorganisms. 19 It has potent proinflammatory properties and contributes directly to tissue injury during the development of laminitis.…”