Effects of ultrasound exposure in vitro on neuroblastoma cell membranes

Fahnestock, Margaret; Rimer, Virginia G.; Yamawaki, Ruth M.; Ross, Pepi; Edmonds, P. D.

doi:10.1016/0301-5629(89)90162-2

Cited by 25 publications

(10 citation statements)

References 16 publications

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“…hydrophilic, molecule that normally crosses the lipid bilayer of the plasma membrane at a slow rate, mainly by passive diffusion (Hecquet et al, 1986). HESW exposure could transiently permeabilise the cell membrane, thus reducing the diffusion barrier for CDDP and increasing CDDP influx in analogy with results obtained by ultrasQund exposure (Ellwart et al, 1987;Kober et al, 1989;Fahnestock et al, 1989) or electropermeabilisation of cells (Melvik et al, 1986). This interpretation seems reasonable regarding the enhanced proliferation capacity following a second HESW administration after concomitant CDDP/HESW treatment.…”

Section: Discussionsupporting

confidence: 71%

The Combined Effects of High Energy Shock Waves and Cytostatic Drugs or Cytokines on Human Bladder Cancer Cells

Wörle

Steinbach

Hofstädter

1993

Ultrasonics International 93

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Section: Discussionsupporting

confidence: 71%

The Combined Effects of High Energy Shock Waves and Cytostatic Drugs or Cytokines on Human Bladder Cancer Cells

Wörle

Steinbach

Hofstädter

1993

Ultrasonics International 93

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“…Knowledge of the effectiveness of US was traditionally based on studies on tissue repair in animals, 10,11 and to a large extent, on in vitro studies. [12][13][14][15] Moreover, a look at the information currently available online on the supposed benefi ts of US therapy for musculoskeletal disorders cites stimulation of blood fl ow to promote healing, relieve pain and infl ammation, reduce muscle spasms, and increase the range of motion. 16 However, a good number of studies on the clinical effectiveness of US in physical therapy revealed contrasting results.…”

Section: Physical Therapymentioning

confidence: 99%

Therapeutic potential of low-intensity ultrasound (part 1): thermal and sonomechanical effects

et al. 2008

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In this first part of the review, we will focus on and discuss various aspects of low-intensity ultrasound (US), with emphasis on mild thermal effects, apoptosis induction, and sonomechanical effects. Mild thermal effects of US have been commonly applied to physical therapy. Though US has clear beneficial effects, the advantage of using US over other heating modalities remains unclear. US has also been used in vivo and clinically in the treatment of wounds and fractures, with promising results. On the biomolecular level, studies have shown that US can induce apoptosis and that certain conditions can provide optimal apoptosis induction. As to potential therapeutic applications, in addition to the thermal and other physical effects, apoptosis induction by US may offer direct and rapid treatment of tumors or cancer tissues. Technological advances and rapidly accelerating research in this field are providing an ever-increasing array of therapeutic options for lowintensity US.

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“…Ultrasound may damage cells irreversibly and induce severe cell membrane modifications (2)(3)(4). The effect of ultrasound on cells has been correlated with the generation of acoustic cavitations (2,5,6).…”

Section: Introductionmentioning

confidence: 99%

Low-frequency low energy ultrasound combined with microbubbles induces distinct apoptosis of A7r5 cells

Zhang

Zhou

Cheng

et al. 2014

Molecular Medicine Reports

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The present study aimed to investigate whether low frequency low energy ultrasound combined with microbubbles induces apoptotic cell death of A7r5 rat aortic vascular smooth muscle cells, and to identify the possible mechanisms underlying this effect. Ultrasonic waves (45 kHz with 0.3 Wcm2 of intensity for 0, 10, 20 and 30 sec) were used together with different dosages of SonoVue™ microbubbles (0, 14, 28, 42 and 56 µl), respectively. The cell viability and apoptotic rate were determined by trypan blue staining immediately following treatment and flow cytometry 24 h thereafter. The treatment conditions resulting in the lowest amount of necrosis, highest apoptotic rate and lowest microbubble dosage was selected for the US+MB group, which was treated with ultrasound combined with microbubbles. The cell proliferation 24 h following treatment was determined and western blot analysis was applied to examine the expression of apoptosis‑associated proteins, B-cell lymphoma 2 (Bcl‑2) and Bcl-2-associated X (Bax). The harmonic acoustic pressure amplitude was measured to obtain the cavitation intensity. The combination of 20 sec ultrasound irradiation and 14 µl SonoVue™ was selected as the treatment conditions for the US+MB group. The results demonstrated that both ultrasound alone (the US group) and in combination with microbubbles significantly inhibited the proliferation of A7r5 cells compared with that of the control (P<0.01), and the suppression in the US+MB group was significantly greater (P<0.01). The apoptotic rate in A7r5 cells induced by this combination treatment (16.62±0.93%) was significantly higher than that in the control (3.93±0.39%; P<0.01) and US (6.88±1.87%; P<0.01) groups. Treatment with ultrasound combined with microbubbles increased the expression of Bax and decreased the ratio of Bcl‑2/Bax compared with those in the control and US groups. The cavitation induced by ultrasound combined with microbubble treatment was more intense than that by ultrasound alone. The results demonstrated that the cell death and apoptosis of A7r5 cells were associated with ultrasound duration and microbubble dosage. Low frequency ultrasound combined with microbubbles induced apoptosis in A7r5 cells through the upregulation of Bax and the downregulation of the Bcl‑2/Bax ratio, where the cavitation effect may have an important role.

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Effects of ultrasound exposure in vitro on neuroblastoma cell membranes

Cited by 25 publications

References 16 publications

The Combined Effects of High Energy Shock Waves and Cytostatic Drugs or Cytokines on Human Bladder Cancer Cells

The Combined Effects of High Energy Shock Waves and Cytostatic Drugs or Cytokines on Human Bladder Cancer Cells

Therapeutic potential of low-intensity ultrasound (part 1): thermal and sonomechanical effects

Low-frequency low energy ultrasound combined with microbubbles induces distinct apoptosis of A7r5 cells

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