Effects of ultrasonic pretreatment of soybean protein isolate on the binding efficiency, structural changes, and bioavailability of a protein-luteolin nanodelivery system

Sun, Fuwei; Li, Bailiang; Guo, Yanan; Wang, Yichang; Cheng, Tianfu; Yang, Qingyu; Li, Jun; Fan, Zhijun; Guo, Zengwang; Wang, Zhongjiang

doi:10.1016/j.ultsonch.2022.106075

Cited by 56 publications

(33 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the interaction between PC and phlorotannin, which caused fluorescence quenching, the intrinsic fluorescence intensity decreased, suggesting that Trp may have been involved. These results were consistent with earlier research that claimed the presence of phlorotannin exposed Trp residues and caused proteins to unfold, which decreased the fluorescence intensity (31,32).…”

Section: Fluorescence Spectroscopysupporting

confidence: 93%

“…The molecular structure of the protein molecule loosened as a result of the hydrogen bond that stabilized the protein structure being broken, the protein molecule's having less ordered structure, and the PC unfolding. The hydrophobic surface of PC was exposed to phlorotannin, which disrupted the hydrogen bond network there and reduced the amount of α-helix (32). Different studies have reported that the changes in the secondary structures after the interaction between proteins and polyphenols and ultrasound treatment were different (34)(35)(36).…”

Section: Secondary Structure Analysismentioning

confidence: 99%

See 1 more Smart Citation

Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum

Bai

Xie

et al. 2023

Front. Nutr.

View full text Add to dashboard Cite

IntroductionPhycocyanin offers advantageous biological effects, including immune-regulatory, anticancer, antioxidant, and anti-inflammation capabilities. While PC, as a natural pigment molecule, is different from synthetic pigment, it can be easily degradable under high temperature and light conditions.MethodsIn this work, the impact of ultrasound treatment on the complex of PC and phlorotannin structural and functional characteristics was carefully investigated. The interaction between PC and phlorotannin after ultrasound treatment was studied by UV–Vis, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, fourier transform infrared (FTIR) spectroscopy. Additionally, the antioxidant potential and in vitro digestibility of the complexes were assessed.ResultsThe result was manifested as the UV–Vis spectrum reduction effect, fluorescence quenching effect and weak conformational change of the CD spectrum of PC. PC was identified as amorphous based on the X-ray diffraction (XRD) data and that phlorotannin was embedded into the PC matrix. The differential scanning calorimetry (DSC) results showed that ultrasound treatment and the addition of phlorotannin could improve the denaturation peak temperatures (Td) of PC to 78.7°C. In vitro digestion and free radical scavenging experiments showed that appropriate ultrasound treatment and the addition of phlorotannin were more resistant to simulated gastrointestinal conditions and could improve DPPH and ABTS+ free radical scavenging performance.DiscussionUltrasound treatment and the addition of phlorotannin changed the structural and functional properties of PC. These results demonstrated the feasibility of ultrasound-assisted phlorotannin from A. nodosum in improving the functional properties of PC and provided a possibility for the application of PC-polyphenol complexes as functional food ingredients or as bioactive materials.

show abstract

Section: Fluorescence Spectroscopysupporting

confidence: 93%

Section: Secondary Structure Analysismentioning

confidence: 99%

Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum

Bai

Xie

et al. 2023

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…In the ultrasound environment, the exposition of hydrophobic groups may enhance the adsorption of polyphenols by proteins. [ 29 ]. Polyphenols were more likely to form covalent bonds with hydrophobic proteins in oxidizing environments (e.g., alkaline environments, or those with free radicals or enzymes).…”

Section: Resultsmentioning

confidence: 99%

Sonochemical Effects on the Preparation, Structure and Function of Gliadin-(−)-Epigallo-Catechin 3-Gallate Conjugates

Cao

Zhang

et al. 2023

Foods

View full text Add to dashboard Cite

It is essential to understand the mechanism of action of ultrasound synergistic free radical oxidation to promote covalent reactions between proteins and polyphenols. (−)-epigallo-catechin 3-gallate (EGCG) with rich bioactivity could be used to increase the functional properties of cereal protein—gliadin (GL). This study systematically explored the role of ultrasound treatment (US) on the binding mechanisms of GL and EGCG. Electrophoresis and high-performance liquid chromatography (HPLC) confirmed the greater molecular mass of the covalent complexes in the ultrasound environment. Quantitative analysis by the phenol content revealed that the ultrasound environment increased the EGCG content in the covalent complex by 15.08 mg/g of protein. The changes in the spatial structure of the proteins were indicated by Fourier infrared and ultraviolet spectroscopy. Additionally, scanning electron microscopy (SEM) and atomic force microscopy (AFM) found that US disrupted the aggregation of GL and the clustered structure of the covalent complexes. The results demonstrated that the water solubility of ultrasonic conjugates was significantly increased by 8.8–64.19%, the digestion rate was more efficient, and the radical scavenging capacity was twice that of GL. This research contributes to the theoretical basis for broadening the application of polyphenols in modifying protein.

show abstract

“…In one of the recent studies, a soy protein isolate pretreated by ultrasonication was selected as the embedding wall material, which was combined with luteolin to form a soy protein isolate (SPI)-luteolin nanodelivery system. This SPI delivery system increased the luteolin release rate and the utilization of fat-soluble active substances [ 116 ]. Another recent report investigated the preparation and evaluation of a non-invasive intranasal luteolin delivery for the management of cognitive dysfunction in Alzheimer’s disease (AD), using novel chitosan-decorated nanoparticles.…”

Section: Insight Into the Nanodelivery Of Luteolin In Cancermentioning

confidence: 99%

Luteolin, a Potent Anticancer Compound: From Chemistry to Cellular Interactions and Synergetic Perspectives

Tuli

Rath

Chauhan

et al. 2022

Cancers

View full text Add to dashboard Cite

Increasing rates of cancer incidence and the toxicity concerns of existing chemotherapeutic agents have intensified the research to explore more alternative routes to combat tumor. Luteolin, a flavone found in numerous fruits, vegetables, and herbs, has exhibited a number of biological activities, such as anticancer and anti-inflammatory. Luteolin inhibits tumor growth by targeting cellular processes such as apoptosis, cell-cycle progression, angiogenesis and migration. Mechanistically, luteolin causes cell death by downregulating Akt, PLK-1, cyclin-B1, cyclin-A, CDC-2, CDK-2, Bcl-2, and Bcl-xL, while upregulating BAX, caspase-3, and p21. It has also been reported to inhibit STAT3 signaling by the suppression of STAT3 activation and enhanced STAT3 protein degradation in various cancer cells. Therefore, extensive studies on the anticancer properties of luteolin reveal its promising role in chemoprevention. The present review describes all the possible cellular interactions of luteolin in cancer, along with its synergistic mode of action and nanodelivery insight.

show abstract

Effects of ultrasonic pretreatment of soybean protein isolate on the binding efficiency, structural changes, and bioavailability of a protein-luteolin nanodelivery system

Cited by 56 publications

References 62 publications

Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum

Ultrasound treatment enhanced the functional properties of phycocyanin with phlorotannin from Ascophyllum nodosum

Sonochemical Effects on the Preparation, Structure and Function of Gliadin-(−)-Epigallo-Catechin 3-Gallate Conjugates

Luteolin, a Potent Anticancer Compound: From Chemistry to Cellular Interactions and Synergetic Perspectives

Contact Info

Product

Resources

About