Effects of Ubiquitin-conjugating Enzyme 2C on Invasion, Proliferation and Cell Cycling of Lung Cancer Cells

Tang, Xiaokui; Wang, Kejian; Tang, Yu-Kui; Chen, Li

doi:10.7314/apjcp.2014.15.7.3005

Cited by 15 publications

(8 citation statements)

References 25 publications

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“…In addition, the expression of UBE2C was positively correlated with histological grade, tumor size, clinical stage and lymph node metastasis of BRCA. In vitro experiment in previous articles have also proved that UBE2C could enhance the proliferative, viability and invasive capacity through MTT, colony formation assay and invasion assays in lung cancer and prostate cancer [ 31 , 32 ], which increased the reliability of our result that UBE2C exerted an oncogenic influence on breast cancer cells. There was also a difference between UBE2C expression in different molecular subtypes of breast cancer, among which the positive rate of UBE2C expression in HER2-overexpression BRCA was the highest, while the positive expression rate of UBE2C was the lowest in Luminal A BRCA.…”

Section: Discussionsupporting

confidence: 69%

The clinicopathological significance of UBE2C in breast cancer: a study based on immunohistochemistry, microarray and RNA-sequencing data

Zhu

et al. 2017

Cancer Cell Int

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BackgroundUbiquitin-conjugating enzyme E2C (UBE2C) has been previously reported to correlate with the malignant progression of various human cancers, however, the exact molecular function of UBE2C in breast carcinoma (BRCA) remained elusive. We aimed to investigate UBE2C expression in BRCA and its clinical significance.MethodsThe expression of UBE2C in 209 BRCA tissue samples and 53 adjacent normal tissue samples was detected using immunohistochemistry. The clinical role of UBE2C was analyzed. Public databases including the human protein atlas and Oncomine were used to assess UBE2C expression in BRCA. Moreover, the cancer genome atlas (TCGA) database was employed to investigate the prognostic value of UBE2C in BRCA.ResultsThe positive expression rate of UBE2C in BRCA was 70.8% (148/209), and UBE2C expression in the adjacent breast tissue was negative. The expression of UBE2C was positively correlated with tumor size (r = 0.32, P < 0.001), histological grade (r = 0.237, P = 0.001), clinical stage (r = 0.198, P = 0.004), lymph node metastasis (r = 0.155, P = 0.026), HER2 expression level (r = 0.356, P < 0.001), Ki-67 expression level (r = 0.504, P < 0.001), and P53 expression level (r = 0.32, P = 0.001). Negative correlations were found between UBE2C expression and the ER (r = − 0.403, P < 0.001) and PR (r = − 0.468, P < 0.001) status. UBE2C gene expression data from the public databases all proved that UBE2C was overexpressed in BRCA. According to the TCGA data analysis, a higher positive expression of UBE2C was associated with worse survival of BRCA patients (P = 0.0428), and data from cBioPortal indicated that 11% of all sequenced BRCA patients possessed a gene alteration of UBE2C, predominately gene amplification and mRNA regulation.ConclusionUbiquitin-conjugating enzyme E2C might pose an oncogenic effect on the progression of BRCA.

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Section: Discussionsupporting

confidence: 69%

The clinicopathological significance of UBE2C in breast cancer: a study based on immunohistochemistry, microarray and RNA-sequencing data

Zhu

et al. 2017

Cancer Cell Int

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“…UBE2C is one of the genes used in molecular classification in several types of cancers. Studies revealed that overexpression of UBE2C is related to some kinds of solid tumors, such as stomach cancer (Zhang H. Q. et al, 2018 ), colorectal cancer (Cacciola et al, 2016 ; Zhang Y. et al, 2018 ), esophageal cancer (Matsumoto et al, 2014 ; Li et al, 2018 ), pancreatic cancer (Zhao et al, 2013 ), breast cancer (Mo et al, 2017 ; Qin et al, 2017 ), ovarian cancer (Martínez-Canales et al, 2018 ), hepatocellular cancer (Ieta et al, 2007 ), lung cancer (Tang et al, 2014 ; Zhang et al, 2015 ), prostate cancer (Shuliang et al, 2013 ), and other cancers (Wagner et al, 2004 ; Shen et al, 2013 ; Kraft et al, 2017 ). Therefore, UBE2C has been considered as an important functional gene in cancer development.…”

Section: Discussionmentioning

confidence: 99%

UBE2C Is a Potential Biomarker of Intestinal-Type Gastric Cancer With Chromosomal Instability

Zhang

Liu

et al. 2018

Front. Pharmacol.

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This study explored potential biomarkers associated with Lauren classification of gastric cancer. We screened microarray datasets on gastric cancer with information of Lauren classification in gene expression omnibus (GEO) database, and compared differentially expressing genes between intestinal-type or diffuse-type gastric cancer. Four sets of microarray data (GSE2669, GSE2680, GDS3438, and GDS4007) were enrolled into analysis. By differential gene analysis, UBE2C, CDH1, CENPF, ERO1L, SCD, SOX9, CKS1B, SPP1, MMP11, and ANLN were identified as the top genes related to intestinal-type gastric cancer, and MGP, FXYD1, FAT4, SIPA1L2, MUC5AC, MMP15, RAB23, FBLN1, ANXA10, and ADH1B were genes related to diffuse-type gastric cancer. We comprehensively validated the biological functions of the intestinal-type gastric cancer related gene UBE2C and evaluated its clinical significance on 1,868 cases of gastric cancer tissues from multiple medical centers of Shanghai, China. The gain of copy number on 20q was found in 4 out of 5 intestinal-type cancer cell lines, and no similar copy number variation (CNV) was found in any diffuse-type cancer cell line. Interfering UBE2C expression inhibited cell proliferation, migration and invasion in vitro, and tumorigenesis in vivo. Knockdown of UBE2C resulted in G2/M blockage in intestinal-type gastric cancer cells. Overexpression of UBE2C activated ERK signal pathway and promoted cancer cell proliferation. U0126, an inhibitor of ERK signaling pathway reversed the oncogenic phenotypes caused by UBE2C. Moreover, overexpression of UBE2C was identified in human intestinal-type gastric cancer. Overexpression of UBE2C protein predicted poor clinical outcome. Taken together, we characterized a group of Lauren classification-associated biomarkers, and clarified biological functions of UBE2C, an intestinal-type gastric cancer associated gene. Overexpression of UBE2C resulted in chromosomal instability that disturbed cell cycle and led to poor prognosis of intestinal-type gastric cancer.

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“…KIF20A promoted cell proliferation and inhibit apoptosis in LUAD [45]. And UBE2C was found to enhance lung cancer growth [46]. Subsequently, we predicted 30 DElncRNAs and 35 DEmiRNAs, in which 7 DEmiRNAs and 7 DElncRNAs functioned as prognostic biomarkers in LUAD.…”

Section: Discussionmentioning

confidence: 87%

“…KIF20A promoted cell proliferation and inhibit apoptosis in LUAD 45 . And UBE2C was found to enhance lung cancer growth 46 .…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of TPX2-related ceRNA network as prognostic biomarkers in lung adenocarcinoma

Chen¹,

Zhang²,

Li³

et al. 2020

Int. J. Med. Sci.

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Background and aim: Competing endogenous RNA (ceRNA) is believed to play vital roles in tumorigenesis. The goal of this study was to screen prognostic biomarkers in lung adenocarcinoma (LUAD). Methods: Common differentially expressed genes (DEGs) were collected from Gene Expression Omnibus (GEO) databases and The Cancer Genome Atlas databases (TCGA) using GEO2R and "limma" package in R, respectively. Overlapping DEGs were conducted using enrichment of functions and protein-protein interaction (PPI) network to discover significant candidate genes. By using a comprehensive analysis, we constructed an mRNA mediated ceRNA network. Survival rates were used Kaplan-Meier analysis. Statistical analysis was used to further identify the prognosis of studied genes. Results: Integrated analysis of GSE32863 and TCGA databases, a total of 886 overlapping DEGs, including 279 up-regulated and 607 down-regulated genes were identified. Considering the highest term of candidate genes in PPI, we identified TPX2, which was enriched in cell division signaling pathway. Besides, 35 differentially expressed miRNAs (DEmiRNAs) were predicted to target TPX2 and only 7 DEmiRNAs were identified to be prognostic biomarkers in LUAD. Then, 30 differentially expressed lncRNAs (DElncRNAs) were predicted to bind these 7 DEmiRNAs. Finally, we found that 7 DElncRNAs were correlated with the overall survival (all p <0.05). Furthermore, we identified elevated TPX2 was strongly correlated with the worse survival rate among 458 samples. Univariate and multivariate cox analysis showed TPX2 may act as an independent factor for prognosis in LUAD (p <0.05). Then pathway enrichment results suggested that TPX2 may facilitate tumorigenesis by participating in several cancer-related signaling pathways in LUAD, especially in Notch signal pathway. Conclusions: TPX2-related lncRNAs and miRNAs are related to the survival of LUAD. 7 lncRNAs, 7 miRNAs and TPX2 may serve as prognostic biomarkers in LUAD.

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Effects of Ubiquitin-conjugating Enzyme 2C on Invasion, Proliferation and Cell Cycling of Lung Cancer Cells

Cited by 15 publications

References 25 publications

The clinicopathological significance of UBE2C in breast cancer: a study based on immunohistochemistry, microarray and RNA-sequencing data

The clinicopathological significance of UBE2C in breast cancer: a study based on immunohistochemistry, microarray and RNA-sequencing data

UBE2C Is a Potential Biomarker of Intestinal-Type Gastric Cancer With Chromosomal Instability

Comprehensive analysis of TPX2-related ceRNA network as prognostic biomarkers in lung adenocarcinoma

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