Comprehensive analysis of TPX2-related ceRNA network as prognostic biomarkers in lung adenocarcinoma

Chen, Huo; Zhang, Meng-Yu; Li, Rui; Zhou, Xi-Jia; T, Liu; Li, Jianping; Liu, Xiao; Qu, Yi-Qing

doi:10.7150/ijms.49053

Cited by 8 publications

(9 citation statements)

References 63 publications

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“…Long noncoding RNAs (lncRNAs) longer than 200 bp can interact with mRNAs, miRNAs, and circulating RNAs to participate in a complex network of gene expression regulation. 4 Insights into the function of a few characteristic lncRNAs suggested a surprising diversity of cellular processes, from chromatin modification, transcription, splicing, and translation to cell differentiation, cell cycle regulation, and stem cell reprogramming. 5 lncRNAs are involved in the development of some cancers.…”

Section: Introductionmentioning

confidence: 99%

LncRNA ADAMTS9-AS2 is a Prognostic Biomarker and Correlated with Immune Infiltrates in Lung Adenocarcinoma

Lin¹,

Huang²,

Yi³

et al. 2021

IJGM

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Section: Introductionmentioning

confidence: 99%

LncRNA ADAMTS9-AS2 is a Prognostic Biomarker and Correlated with Immune Infiltrates in Lung Adenocarcinoma

Lin¹,

Huang²,

Yi³

et al. 2021

IJGM

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“…Previously, numerous studies have analyzed the association between TPX2 and human malignancy (12)(13)(14). To the best of our knowledge, the present study is the first to focus on TPX2 function in osteosarcoma.…”

Section: Discussionmentioning

confidence: 88%

“…A number of studies have reported that the activation of TPX2 is associated with a variety of different cancer types (12)(13)(14)(15), but the molecular mechanisms underlying TPX2 function in osteosarcoma are not well understood.…”

Section: Discussionmentioning

confidence: 99%

“…Upregulation of TPX2 can cause centrosome amplification and lead to DNA polyploidy, thus regulating cell proliferation, apoptotic processes and cell division (10). Upregulation of TPX2 has been reported in multiple types of cancer, including colon, lung, pancreatic, cervical and salivary gland cancer (11)(12)(13)(14)(15). These observations indicate that TPX2 plays a pivotal role in the oncogenesis of malignancies.…”

Section: Introductionmentioning

confidence: 99%

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TPX2 regulated by miR‑29c‑3p induces cell proliferation in osteosarcoma via the AKT signaling pathway

Zhu

Zhang

et al. 2022

Oncol Lett

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The present study aimed to investigate the significance of targeting protein for Xenopus kinesin-like protein 2 (TPX2) expression in osteosarcoma. First, the TPX2 expression and survival analysis data were evaluated from The Cancer Genome Atlas (TCGA) database. Next, reverse transcription-quantitative PCR was used to explore the expression of TPX2 in osteosarcoma tissues. The observed potential target relationship between TPX2 and microRNA (miR)-29c-3p was verified using TargetScan and luciferase reporter assays. Kaplan-Meier survival analysis was used to determine associations between TPX2 expression levels and survival prognosis. TPX2 small interfering RNA was successfully constructed and transfected into osteosarcoma cell lines. The effects of TPX2 on osteosarcoma cell proliferation were then detected by MTT assay. In addition, the expression levels of AKT signaling pathway-associated proteins were identified by western blot analysis. The expression of TPX2 was upregulated and the expression of miR-29c-3p was downregulated in osteosarcoma. High expression of TPX2 was linked to a poor prognosis. Using luciferase assay and the miRNA mimic and inhibitors, miR-29c-3p was able to target and repress TPX2, and siRNA knockdown of TPX2 resulted in the inhibition of osteosarcoma cell proliferation by affecting the AKT pathway. Overall, the study showed that miR-29c-3p could inhibit the proliferation of osteosarcoma cells via TPX2 downregulation, and that TPX2 and miR-29c-3p may serve as promising prognostic indicators.

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“…In recent years, the ceRNA network has been widely studied and it has been shown that ceRNA affects the development of numerous cancers, for example, lung cancer, 93 , 94 breast cancer, 95 and gastric cancer. 32 It was also studied in COPD, 35 but it was deficient.…”

Section: Discussionmentioning

confidence: 99%

Autophagy Induced by BCL2-Related ceRNA Network Participates in the Occurrence of COPD

Shi¹,

Zhang²,

Liu³

et al. 2022

COPD

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Purpose Chronic obstructive pulmonary disease (COPD) is a predominant cause of mortality worldwide. Autophagy, which depends on a lysosomal degradation pathway, plays an essential role in the occurrence of COPD. The aim of our study was to identify the potential function of autophagy and construct a BCL2-related competing endogenous RNA (ceRNA) network that induces autophagy in COPD. Methods Blood sample data from GSE31568, GSE24709, and GSE61741 were collected from the Gene Expression Omnibus (GEO) database. Differentially expressed miRNAs in COPD and controls were identified via GEO2R. Transcription factors were obtained from FunRich. DIANA, miRDB, miRTarBase, and TargetScan were used to predict target genes of miRNAs. Autophagy genes were collected from the Human Autophagy Database (HADb). The GSE151052 dataset was used to identify autophagy-related differentially expressed genes in tissues. Functional enrichment and protein–protein interaction (PPI) network analyses were conducted via Metascape and the STRING network. Spearman correlation analysis was used to analyze the relationship between autophagy-related differentially expressed genes and lung function. The BCL2-related ceRNA network was modeled by Cytoscape. Results We obtained 41 differentially expressed miRNAs and 10 significantly different transcription factors. We identified 19 autophagy-related differentially expressed genes that were significantly different ( P <0.05) in tissue samples. The most significant enrichment in Metascape was an autophagy item, which further confirmed autophagy participation in the occurrence of COPD. PPI network analysis found four genes (BCL2, BECN1, MAPK8, and ITPR1), among which BCL2 was correlated with both FEV1/FVC and FEV1 prediction. Finally, the BCL2-related ceRNA network was constructed to clarify the interaction of RNAs and occurrence of autophagy, including 18 miRNAs and 65 lncRNAs. Conclusion We identified 19 autophagy-related differentially expressed genes that participated in COPD; among them, BCL2 was correlated with lung function, and a BCL2-related ceRNA network was constructed, which further revealed the potential mechanism of autophagy involvement in COPD.

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Comprehensive analysis of TPX2-related ceRNA network as prognostic biomarkers in lung adenocarcinoma

Cited by 8 publications

References 63 publications

LncRNA ADAMTS9-AS2 is a Prognostic Biomarker and Correlated with Immune Infiltrates in Lung Adenocarcinoma

LncRNA ADAMTS9-AS2 is a Prognostic Biomarker and Correlated with Immune Infiltrates in Lung Adenocarcinoma

TPX2 regulated by miR‑29c‑3p induces cell proliferation in osteosarcoma via the AKT signaling pathway

Autophagy Induced by BCL2-Related ceRNA Network Participates in the Occurrence of COPD

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