Effects of Typified Propolis on Mutans Streptococci and Lactobacilli: a Randomized Clinical Trial

Abstract

Objective-The aim of this study was to determine in a randomized, double-blind, placebocontrolled clinical trial the effects of typified propolis and chlorhexidine rinses on salivary levels of mutans streptococci (MS) and lactobacilli (LACT).Methods-One hundred patients were screened for salivary levels of MS >100,000 CFUs/mL of saliva. All patients presented with at least one cavitated decayed surface. Sixty patients met entry criteria. Subjects were adults 18-55 years old. After restoration of cavitated lesions patients were randomized to 3 experimental groups: 1) PROP-alcohol-free 2% typified propolis rinse (n = 20); 2) CHX-0.12% chlorhexidine rinse; 3) PL-placebo mouthrinse. Patients rinsed unsupervised 15 mL of respective rinses twice a day for 1 min for 28 days. Patients were assessed for the salivary levels of MS (Dentocult SM) and LACT (Dentocult LB) at baseline, 7-day, 14-day, and at 28-day visits (experimental effects) and at 45-day visit (residual effects). General linear models were employed to analyze the data.Results-PROP was superior to CHX at 14-day and 28-day visits in suppressing the salivary levels of MS (p < .05). PROP was superior to PL at all visits (p < .01). The residual effects of PROP in suppressing the salivary levels of MS could still be observed at the 45-day visit, where significant differences between PROP and CHX (p < .05), were demonstrated. PROP was significantly superior than CHX in suppressing the levels of salivary LACT at the 28-day visit (p < .05).Conclusion-Typified propolis rinse was effective in suppressing cariogenic infections in caries-active patients when compared to existing and placebo therapies.

The Assessment of Oral Microflora Exposed to 3% Ethanolic Extract of Brazilian Green Propolis Preparation Used for Hygiene Maintenance following Minor Oral Surgeries

“…Al-Sultan et al concluded that an aqueous extract of propolis as a topical agent following lower third molar extraction had a slight reducing effect on the severity of postoperative complications [ 39 ]. It was observed that Brazilian propolis mouthrinse was effective in suppressing cariogenic infections as well as reducing gingival inflammation [ 40 , 41 ]. There is a granted patent in Brazil about products elaborated with Brazilian green propolis for use in dentistry [ 42 ].…”

Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent

“…Useful knowledge about propolis has been gathered from research in the fields of medicine, pharmacology, food sciences and chemistry [ 2 , 3 ]. In dentistry, some work has been published on endodontics [ 4 , 5 ], preventive dentistry [ 6 , 7 ], cariology [ 3 , 6 ], surgery [ 8 ], and periodontics [ 9 , 10 ], but there is a shortage of studies in the field of restorative dentistry.…”

“…Brazilian RP has potent antimicrobial activity even at low concentrations (0.1 and 1.0%). Several studies have proved the effective action of Brazilian RP against Streptococcus mutans and Lactobacillus [ 3 , 7 , 17 – 19 ] attributed to the high concentration of flavonoids and phenolic compounds in Brazilian RP [ 20 ]. The best results were obtained with an acetone fraction of an alcoholic extract of Brazilian propolis, possibly because of its high polarity; the acetone fraction contains a greater amount of active phenolics, which increases the antimicrobial activity [ 21 ].…”

Oral Health of Patients Treated with Acrylic Partial Dentures Using a Toothpaste Containing Bee Product

“…Another study from 2013 compared 0.12% chlorhexidine with propolis at a much lower concentration, namely, 2%. Its antimicrobial activity against S. mutans and L. acidofilus was confirmed [ 43 ]. These studies proved the cariostatic properties of propolis, antiperiodontal pathogen activity, and efficiency at lower concentrations.…”