Effects of type III antifreeze protein on sperm and embryo cryopreservation in rabbit

Nishijima, Ken‐ichi; Tanaka, Mai; Sakai, Yusuke; Koshimoto, Chihiro; Morimoto, Masatoshi; Watanabe, Teruo; Fan, Jianglin; Kitajima, Shuji

doi:10.1016/j.cryobiol.2014.04.014

Cited by 49 publications

(25 citation statements)

References 28 publications

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“…Some of the cell types tested for cryopreservation with the addition of AFPs include sperms [167,168,169,170,171,176,178,200], oocytes [66,70,83,177,181,183,185,190,198,199], human liver cells [173], RIN-5F insulin tumor cells [174], diatoms [143], red blood cells [18,144,197], muscle cells [162,179], gut cell [188], islet cells [193], E. coli [83], and human cell lines [145] including HeLa cells, NIH/3T3 cells, preosteoblasts (MC3T3-E1 cells), and human ketatinocytes (HaCaT cells). Thus, the addition of AFPs seems to mainly enhance the cryopreservation efficiency regardless of cell type and freezing method, with a handful of exceptions [68,89,90,162,164].…”

Section: Cryopreservation Using Afps As a Potential Cryoprotectantmentioning

confidence: 99%

“…Embryos from fish [166,186,187,196,202], cows [172,203], sheep [175], rabbits [176], mice [67,92,183,191], and horses [91] were preserved in the presence of AFPs. Early attempts with equine and mouse embryos demonstrated that fish AFPs had negligible effects [91,92]; however, fish embryos subjected to microinjection or incubation in type I AFP solution showed significantly increased survival after chilling at 4 °C or −10 °C.…”

Section: Cryopreservation Using Afps As a Potential Cryoprotectantmentioning

confidence: 99%

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Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant

et al. 2017

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Antifreeze proteins (AFPs) are biological antifreezes with unique properties, including thermal hysteresis (TH), ice recrystallization inhibition (IRI), and interaction with membranes and/or membrane proteins. These properties have been utilized in the preservation of biological samples at low temperatures. Here, we review the structure and function of marine-derived AFPs, including moderately active fish AFPs and hyperactive polar AFPs. We also survey previous and current reports of cryopreservation using AFPs. Cryopreserved biological samples are relatively diverse ranging from diatoms and reproductive cells to embryos and organs. Cryopreserved biological samples mainly originate from mammals. Most cryopreservation trials using marine-derived AFPs have demonstrated that addition of AFPs can improve post-thaw viability regardless of freezing method (slow-freezing or vitrification), storage temperature, and types of biological sample type.

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Section: Cryopreservation Using Afps As a Potential Cryoprotectantmentioning

confidence: 99%

Section: Cryopreservation Using Afps As a Potential Cryoprotectantmentioning

confidence: 99%

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant

et al. 2017

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“…In ram, AFP type I and antifreeze glycoproteins (AFGP) type I enhanced post-thaw motility when used at 10 µg/mL concentration [28]. Recently, the positive effect of AFPs on sperm motility after thawing, specifically of AFPIII at 1 µg/mL, has been demonstrated in other species such as the Japanese white rabbit [29]. In the case of buffalo, different articles have been published trying to improve sperm freezability by AFP addition in the cryoprotectant medium [30,31,32].…”

Section: Use Of Antifreeze Proteins In Sperm Cryopreservationmentioning

confidence: 99%

The Use of Antifreeze Proteins in the Cryopreservation of Gametes and Embryos

2019

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The cryopreservation of gametes and embryos is a technique widely used in reproductive biology. This technology helps in the reproductive management of domesticated animals, and it is an important tool for gene banking and for human-assisted reproductive technologies. Antifreeze proteins are naturally present in several organisms exposed to subzero temperatures. The ability for these proteins to inhibit ice recrystallization together with their ability to interact with biological membranes makes them interesting molecules to be used in cryopreservation protocols. This mini-review provides a general overview about the use of antifreeze proteins to improve the short and long term storage of gametes and embryos.

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“…Progenitor (stem) cells remain challenging to cryopreserve, requiring large amounts of organic solvents which give less than 100% cell recovery and can affect their future differentiation pathways 15 . AF(G)Ps are not always suitable for cryopreservation due to potential immunogenicity and toxicity concerns 16 , expense of production and the formation of needle like ice crystals (dynamic ice shaping), which is undesirable for cell cryopreservation, leading to mixed successes and failures 12 , 13 , 17 . There is a clear rationale to develop synthetic mimics with tunable and tailored function.…”

Section: Introductionmentioning

confidence: 99%

Polymer mimics of biomacromolecular antifreezes

et al. 2017

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Antifreeze proteins from polar fish species are remarkable biomacromolecules which prevent the growth of ice crystals. Ice crystal growth is a major problem in cell/tissue cryopreservation for transplantation, transfusion and basic biomedical research, as well as technological applications such as icing of aircraft wings. This review will introduce the rapidly emerging field of synthetic macromolecular (polymer) mimics of antifreeze proteins. Particular focus is placed on designing polymers which have no structural similarities to antifreeze proteins but reproduce the same macroscopic properties, potentially by different molecular-level mechanisms. The application of these polymers to the cryopreservation of donor cells is also introduced. Water is fundamental to all life on our planet, and despite it having a freezing point of 0˚C, Nature has evolved a series of unique adaptations to enable life to flourish in sub-zero climates, at high altitudes and at the Earth's poles. Such extremophiles include the wood frog (Lithobates sylvaticus) which can freeze solid over winter, tardigrades which can be desiccated and rehydrated and cold tolerant plants 1, 2 . The mechanisms of these cryoprotectants are varied, from enabling freeze-tolerance (being able to be frozen and then thawed) to freeze avoidance (preventing ice forming) and even freeze promotion (as a predatory mechanism) 3-6 .One particular adaptation is the production of macromolecular antifreezes (proteins and polysaccharides) which modulate ice formation and growth, and are found in freeze avoidant organisms. These can be broadly split into the antifreeze proteins (AFPs) and antifreeze glycoproteins (AFGPs). AFGPs are highly conserved, with a relatively simple repeat tripeptide structure and a disaccharide on every third amino acid, but are produced in a range of chain lengths. Conversely, AFPs are far more diverse, with several subdivisions, and can assume different structures; from beta barrels to alpha helices and vary in size (Fig. 1). They all have a few core properties (discussed in detail below) including the ability to inhibit ice recrystallization, shape ice crystals into unusual morphologies and to depress the freezing point in a noncolligative manner. The relative magnitude of each effect varies between individual AF(G)Ps and the exact mechanisms, involving ice-face recognition, are still under investigation. What is clear, is that the ability to tune and modify ice growth and formation has the potential for huge industrial and societal impact. For example, ice adhesion limits the performance of wind farms by up to 50% 7 , is a major problem for aircraft 8 and even impacts our understanding of how biological components affect our climate 9, 10 .Some AFPs have already found application in improving the texture of ice cream products by preventing ice crystal growth, and other food uses are under investigation 4,11 . A key potential field where AF(G)Ps could be applied is in cell and tissue cryopreservation where ice

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Effects of type III antifreeze protein on sperm and embryo cryopreservation in rabbit

Cited by 49 publications

References 28 publications

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant

The Use of Antifreeze Proteins in the Cryopreservation of Gametes and Embryos

Polymer mimics of biomacromolecular antifreezes

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