Effects of two medicinal plants Psidium guajava L. (Myrtaceae) and Diospyros mespiliformis L. (Ebenaceae) leaf extracts on rat skeletal muscle cells in primary culture

Abstract

Crude decoction, aqueous and ethanolic extracts of two medicinal plants (Psidium guajava and Diospyros mespiliformis), widely used in the central plateau of Burkina Faso to treat many diseases were evaluated for their antagonistic effects on caffeine induced calcium release from sarcoplasmic reticulum of rat skeletal muscle cells. These different extracts showed a decrease of caffeine induced calcium release in a dose dependent manner. Comparison of the results showed that Psidium guajava leaf extracts are more active than extracts of Diospyros mespiliformis and that crude decoctions show better inhibitory activity. The observed results could explaine their use as antihypertensive and antidiarrhoeal agents in traditional medicine, by inhibiting intracellular calcium release.

Phytopharmacological overview of Psidium guajava Linn.

“…25 The in vitro antibacterial activity of P. guajava leaf extracts of S. aureus is possibly due to protein degrading activity of the extracts. 26 P. guajava leaves flavonoids are also shown to have bacteriostatic effects on fish pathogenic bacteria. 27 P. guajava and Azadirachta indica extracts showed higher antimicrobial activity food borne pathogens of Gram-positive bacteria than Gram-negative bacteria (except for Vibrio parahaemolyticus, Pseudomonas aeroginosa, and Acromonas hydrophila.…”

“…The numerous phytoconstituents such as, polyphenolic compounds, flavonoids, pentacyclic triterpenoids, quercetin, guiajaverin, tannins, and other chemical compounds present in the plant are considered for the observed hypotensive effects of the plant leaf extract. 84 Belemtougri et al 85 evaluated P. guajava and Diospyros mespiliformis (crude decoction, aqueous and ethanolic extracts) for their antagonistic activities on caffeine induced release of calcium from the sarcoplasmic reticulum of skeletal muscle in rats. These different extracts have shown a decrease of caffeine induced calcium release in a dose-dependent manner.…”

Evaluation de la toxicité d’un extrait aqueux de <i>Sacoglottis gabonensis</i> (Baille) Urban (Humiriaceae) chez les rongeurs, une plante utilisée dans le traitement de l’ulcère de Buruli en Côte d’Ivoire

“…Le tri-phytochimique a été effectué sur les extraits aqueux, éthérique et méthanolique selon les méthodes utilisées par Belemtougri et al (2006). Deux (2) g de lyophilisat ont été dissous dans 50 mL d'eau distillée pour constituer l'extrait aqueux de Sacoglottis gabonensis.…”

Antidiarrheal Activity ofLithocarpus dealbata. andUrena lobata. Extracts: Therapeutic Implications

“…These tannins precipitate proteins of enterocytes, which in turn reduce the peristaltic movements and intestinal secretion (Almeida et al, 1995). Studies on the functional role of tannins also reveal that they could also bring similar functions by reducing the intracellular Ca 2þ inward current or by activation of the calcium pumping system (which induces the muscle relaxation) (Belemtougri et al, 2006). It seems that the antidiarrheal effect of L. dealbata may be the result of similar mechanisms.…”