Effects of two diluents in the Microtox® toxicity bioassay with marine sediments

Onorati, Fulvio; Mecozzi, Mauro

doi:10.1016/j.chemosphere.2003.09.010

Cited by 55 publications

(35 citation statements)

References 12 publications

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“…Moreover, the addition of a variety of chemicals are known to affect the cellular respiration by the modification of both protein and lipid biosynthetic pathways, altering the luminescence emission; i.e., in the presence of pollutants, the natural bioluminescence of Vibrio fischeri decreases (Onorati and Mecozzi 2004).…”

Section: Levulinate Toxicity In Bacteriamentioning

confidence: 99%

Ecotoxicity studies of the levulinate ester series

et al. 2014

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Abstract:The increasing interest in the development of novel green solvents has led to the synthesis of benign alternative products with minimized environmental impacts.However, most of published studies on green solvents focus primarily on their physicochemical properties, with limited emphasis on absence of ecotoxicological assessment. In this study, we evaluated the acute ecotoxicity of four levulinates (levulinic acid, methyl levulinate, ethyl levulinate and butyl levulinate) on freshwater algae (Chlamydomonas reinhardtii), bacteria (Vibrio fischeri), daphnids (Daphnia magna) and earthworms (Eisenia foetida) using various dose-response tests. As a general trend, the toxicity of levulinate esters in aquatic exposure (assessed as the EC 50 ) increased as a function of increasing alkyl chain length; accordingly, the most toxic compound for the aquatic organisms was butyl levulinate, followed by ethyl levulinate and methyl levulinate. The most toxic compound for Eisenia foetida (terrestrial exposure) was methyl levulinate, followed by ethyl levulinate, butyl levulinate and levulinic acid; in this case, we observed an inverse relationship between toxicity and alkyl chain length. Based on both the lowest EC 50 found in the aquatic media and the ratio between predicted environmental concentration (PEC) and the predicted no-effect concentration (PNEC), we have estimated the maximum allowable values in the 2 environment for these chemicals to be 1.093 mg L -1 for levulinic acid, 2.761 mg L -1 for methyl levulinate, 0.982 mg L -1 for ethyl levulinate and 0.151 mg L -1 for butyl levulinate.

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Section: Levulinate Toxicity In Bacteriamentioning

confidence: 99%

Ecotoxicity studies of the levulinate ester series

et al. 2014

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“…[19] V. fischeri is a marine bacterium which does not represent the behaviour of microorganisms in activated sludge since it is not likely to be found in a WWTP. However, it is widely used because of some advantages derived from sensitivity, reproducibility and ease application with organic and inorganic pollutants, [20] allowing comparisons with other related studies. [21,22] The Microtox® test is the most widely used commercial assay using V. fischeri.…”

Section: Introductionmentioning

confidence: 99%

Assessment of toxicity and biodegradability on activated sludge of priority and emerging pollutants

Tobajas

Verdugo

Polo

et al. 2015

Environmental Technology

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“…El resultado indica que a una mayor concentración hay una menor mortandad de esta bacteria y por lo tanto menor toxicidad de los contaminantes que están presentes en la muestra analizada (Azur 1992, Jennings et al 2001, Hsieha et al 2004, Tsiridis et al 2006. El bioensayo Microtox® ha mostrado mayor sensibilidad y una buena correlación con los resultados obtenidos en peces y en Daphnia magna, siendo desde 1984 una de las pruebas recomendadas por la Agencia de Protección Ambiental (EPA, por sus siglas en inglés) para la evaluación de la toxicidad aguda de muestras de agua (Bennett y Cubbage 1992, Azur 1998, Beelen 2003, Onorati y Mecozzi 2004, Burga-Pérez et al 2012.…”

Section: Introductionunclassified

Evaluación De La Toxicidad De Los Sedimentos Del Curso Alto Del Río Lerma, México

Carreño

Zarazua

Fall

et al. 2018

Rev. Int. Contam. Ambie.

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Palabras clave: microtox®, contaminación en agua RESUMEN El objetivo de este estudio fue determinar la toxicidad de los sedimentos del curso alto del río Lerma (CARL), Estado de México, por medio del ensayo Microtox® con el protocolo básico en la fase sólida. Se colectaron muestras de sedimento superficial en 23 sitios distribuidos sobre el CARL: 12 sobre el cauce del río y 11 en sus tributarios, durante dos campañas de muestreo en un periodo de un año. La concentración efectiva media (EC50) en Vibrio fischeri varió para los sedimentos riverinos entre 139 mg/L y 3588 mg/L y para los de las descargas fue entre 108 mg/L y 16436 mg/L. En general los sedimentos del CARL pueden clasificarse como extremadamente tóxicos, lo cual es un indicativo del impacto de las contribuciones antrópicas de origen doméstico e industrial que recibe este importante cuerpo de agua. Key words: microtox®, water pollution ABSTRACTThe aim of this study was to evaluate the toxicity of sediments in the upper course of the Lerma River (UCLR), in the State of Mexico by means of Microtox® test using the basic solid phase test protocol. The surface sediment samples were collected in 23 sites distributed following the main stream flow of the river: 12 on the river and 11 on its tributaries for two sampling campaigns in a one year period. Half maximal effective concentration (EC50) in Vibrio fischeri varied in the river sediment from 139 mg/L to 3588 mg/L and for the tributaries it varied between 108 mg/L to 16436 mg/L. In general, sediments of the UCLR can be classified as extremely toxics which show the impact of anthropogenic contributions that come from urban and industrial untreated discharges.

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Effects of two diluents in the Microtox® toxicity bioassay with marine sediments

Cited by 55 publications

References 12 publications

Ecotoxicity studies of the levulinate ester series

Ecotoxicity studies of the levulinate ester series

Assessment of toxicity and biodegradability on activated sludge of priority and emerging pollutants

Evaluación De La Toxicidad De Los Sedimentos Del Curso Alto Del Río Lerma, México

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