Effects of triploid induction on innate immunity and hematology in Astyanax altiparanae

Pereira, Newton Narciso; Carriero, Mateus Maldonado; Yasui, George Shigueki; Meira, Caroline Munhoz; Sousa, Ricardo Luiz Moro de; Maia, Antônio Augusto Mendes; Senhorini, José Augusto; Pilarski, Fabiana

doi:10.1016/j.fsi.2021.05.004

Cited by 5 publications

(2 citation statements)

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“…The embryos and adults of yellowtail tetra species were used to evaluate toxicity of cyanopeptides ( Fernandes et al, 2019 ) and aflatoxins ( Michelin et al, 2017 ). An immunological study using triploids was conducted by Levy-Pereira et al (2021) , who observed that the cell counts of erythrocytes, leukocytes, and neutrophils were lower in triploid fish than diploids. Triploid erythrocytes were also larger with higher frequencies of abnormalities.…”

Section: The Use Of Yellowtail Tetra In Other Studiesmentioning

confidence: 99%

Establishing a model fish for the Neotropical region: The case of the yellowtail tetra Astyanax altiparanae in advanced biotechnology

et al. 2022

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The use of model organisms is important for basic and applied sciences. Several laboratory species of fishes are used to develop advanced technologies, such as the zebrafish (Danio rerio), the medaka (Oryzias latipes), and loach species (Misgurnus spp.). However, the application of these exotic species in the Neotropical region is limited due to differences in environmental conditions and phylogenetic distances. This situation emphasizes the establishment of a model organism specifically for the Neotropical region with the development of techniques that may be applicable to other Neotropical fish species. In this work, the previous research efforts are described in order to establish the yellowtail tetra Astyanax altiparanae as a model laboratory species for both laboratory and aquaculture purposes. Over the last decade, starting with artificial fertilization, the yellowtail tetra has become a laboratory organism for advanced biotechnology, such as germ cell transplantation, chromosome set manipulation, and other technologies, with applications in aquaculture and conservation of genetic resources. Nowadays, the yellowtail tetra is considered the most advanced fish with respect to fish biotechnology within the Neotropical region. The techniques developed for this species are being used in other related species, especially within the characins class.

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Section: The Use Of Yellowtail Tetra In Other Studiesmentioning

confidence: 99%

Establishing a model fish for the Neotropical region: The case of the yellowtail tetra Astyanax altiparanae in advanced biotechnology

et al. 2022

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“…15 In addition to enhanced growth, 9 triploid organisms also exhibit increases in expression of genes related to metabolism and growth, resulting in faster growth via increased feeding behaviors. [17][18][19][20] Despite the potential for increased growth, there have been wellsupported negative impacts of triploidy via altered gene expression on the organism's immune system, [21][22][23] survival, 21,23 stress response, 17 neuroendocrine function and neurogenesis effects. 24 To our knowledge, little research focuses on how altered gene expression in triploid fish drives behavioral changes.…”

Section: Introductionmentioning

confidence: 99%

Behavioral transcriptomic effects of triploidy and probiotic therapy (Bifidobacterium, Lactobacillus, and Lactococcus mixture) on juvenile Chinook salmon (Oncorhynchus tshawytscha)

Frank,

Sadeghi,

Heath

et al. 2024

Genes Brain and Behavior

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Aquaculturists use polyploid fish to maximize production albeit with some unintended consequences including compromised behaviors and physiological function. Given benefits of probiotic therapies (e.g., improved immune response, growth, and metabolism), we explored probiotic supplementation (mixture of Bifidobacterium, Lactobacillus, and Lactococcus), to overcome drawbacks. We first examined fish gut bacterial community composition using 16S metabarcoding (via principal coordinate analyses and PERMANOVA) and determined probiotics significantly impacted gut bacteria composition (p = 0.001). Secondly, we examined how a genomic disruptor (triploidy) and diet supplements (probiotics) impact gene transcription and behavioral profiles of hatchery‐reared Chinook salmon (Oncorhynchus tshawytscha). Juveniles from four treatment groups (diploid‐regular feed, diploid‐probiotic feed, triploid‐regular feed, and triploid‐probiotic feed; n = 360) underwent behavioral assays to test activity, exploration, neophobia, predator evasion, aggression/sociality, behavioral sensitivity, and flexibility. In these fish, transcriptional profiles for genes associated with neural functions (neurogenesis/synaptic plasticity) and biomarkers for stress response and development (growth/appetite) were (i) examined across treatments and (ii) used to describe behavioral phenotypes via principal component analyses and general linear mixed models. Triploids exhibited a more active behavioral profile (p = 0.002), and those on a regular diet had greater Neuropeptide Y transcription (p = 0.02). A growth gene (early growth response protein 1, p = 0.02) and long‐term neural development genes (neurogenic differentiation factor, p = 0.003 and synaptysomal‐associated protein 25‐a, p = 0.005) impacted activity and reactionary profiles, respectively. Overall, our probiotic treatment did not compensate for triploidy. Our research highlights novel applications of behavioral transcriptomics for identifying candidate genes and dynamic, mechanistic associations with complex behavioral repertoires.

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Diploid and triploid Chinook salmon (Oncorhynchus tshawytscha) have altered microRNA responses in immune tissues after infection with Vibrio anguillarum

Cadonic,

Heath,

Dixon

et al. 2023

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Effects of triploid induction on innate immunity and hematology in Astyanax altiparanae

Cited by 5 publications

References 46 publications

Establishing a model fish for the Neotropical region: The case of the yellowtail tetra Astyanax altiparanae in advanced biotechnology

Establishing a model fish for the Neotropical region: The case of the yellowtail tetra Astyanax altiparanae in advanced biotechnology

Behavioral transcriptomic effects of triploidy and probiotic therapy (Bifidobacterium, Lactobacillus, and Lactococcus mixture) on juvenile Chinook salmon (Oncorhynchus tshawytscha)

Diploid and triploid Chinook salmon (Oncorhynchus tshawytscha) have altered microRNA responses in immune tissues after infection with Vibrio anguillarum

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