Effects of trimetazidine in acute pancreatitis induced by L-arginine

Abstract

Background:In acute pancreatitis, oxygen free radicals (OFRs) and cytokines have been shown to play a role in the failure of pancreatic microcirculation and the develop ment of local tissue damage. We studied the effects of trimetazidine (TMZ), a potent antioxidant and anti-ischemic agent, on acute pancreatitis.Methods: Rats were randomized into 3 groups: a control group (n = 15), a study group (n = 15) in which acute pancreatitis was induced with with L-arginine, and a treatment group (n = 15) in which pancreatitis was induced and treated with TMZ intraperitoneally. The rats were followed for 24 hours. At the 24th hour we determined serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), amylase, lactate dehydrogenase (LDH), interleukin 1-β (IL-1β), interleukin 6 (IL-6) and tumour necrosis factor-α (TNF-α), and the pancreatic tissues were analyzed histopathologically.

Eff ects of hemin, a heme oxygenase-1 inducer in L-arginine-induced acute pancreatitis and associated lung injury in adult male albino rats

“…Furthermore, TNF-α and IL-1β are especially prominent and are regarded as the "fi rst-line" of cytokines (Akyazi et al 2013). Yenicerioglu et al (2013) have reported that following the intraperitoneal injection of L-arginine, IL-1β, IL-6 and TNF-α, as noticed in our result, have been mentioned to be released from macrophages and monocytes, which are activated as a result of stimulation of peritoneal macrophages or severe damage to the pancreas. Al-Malki (2015) has reported that cytokines are establish to induce an oxidative stress by the production of oxygen free radicals, inducible nitric oxide synthase (iNOS) expression, NO, which serve as a key cellular mediator of infl ammation and superoxide production.…”

Comparing the preventive effect of sodium hydrosulfide, leptin, and curcumin against L-arginine induced acute pancreatitis in rats: role of corticosterone and inducible nitric oxide synthase

“…treated group in our study can be due to enhancement of oxidation of cellular membrane biomolecules including polyunsaturated fatty acids by L-arginine. This could result in formation of MDA, which can activate nuclear factor-kappa B (NF-κB) with a subsequent up regulation of various inflammatory cytokines, which has been found by Yenicerioglu et al (2013). Hasan et al (2015) have also reported that the excess ROS could attack lipids and proteins in the membranes of zymogen granules in pancreas which led to infiltration of great amount of digestive enzymes into pancreatic tissues with subsequent autodigestion and elevation of pancreatic enzymes that is in accordance with our study results.…”

Trimetazidine Increases Cell Survival and Inhibits the Activation of Inflammatory Response in Sodium Taurocholate–Induced Acute Pancreatitis

“…Based on histopathologic assessment, statistically significant differences were detected between the AP and AP þ TMZ groups, and we observed less severity in the TMZ treatment group in terms of leukocyte infiltration, acinar cell necrosis, and apoptotic cell count (P , 0.05). Yenicerioglu et al 19 have observed a histopathologic improvement at 24 hours of TMZ treatment in an L-arginine-induced pancreatitis model, whereas Tanoglu et al 18 have observed the same at 18 hours in a caerulein pancreatitis model. The apoptotic cell counts were reduced in the AP þ TMZ group.…”

“…In the literature, there are 2 reports on caerulein-induced and L-arginine-induced acute experimental pancreatitis treated with TMZ. 18,19 In these studies, however, the abovementioned parameters were not analyzed. In our study, the MDA levels, which are the indicators of cellular membrane damage, regressed upon TMZ treatment to the levels found in the nonpancreatitis groups.…”