Effects of triazole plant growth regulators on molting mechanism in Chinese mitten crab (Eriocheir sinensis)

Li, Changsheng; Huang, Lan; Zhang, Yuting; Guo, Xuanjun; Cao, Niannian; Yao, Chunlian; Duan, Liusheng; Li, Xuefeng; Pang, Sen

doi:10.1016/j.fsi.2022.10.059

Cited by 4 publications

(4 citation statements)

References 42 publications

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“…Therefore, it is often make acquainted with into some parent moieties to enhance the medicinal and biological applications during the design of drug and synthesis of heterocyclic derivatives. Furthermore, triazole is one of the five-membered heterocycles containing nitogen, introduced into compounds that will contribute to a wide range of biological properties including anti-inflammatory [9], anticancer [10,11], antifungal [12], antianxiety [13] insecticidal [14] and plant growth regulating activities [15]. Some medicinal plants such as cyproconazole, triadimefon, metconazole, tebuconazole, propiconazole and epoxiconazole containing triazole moieties (Fig.…”

Section: Synthesis Anticancer Evaluation and Molecular Docking Studie...mentioning

confidence: 99%

Synthesis, Anticancer Evaluation and Molecular Docking Studies of 1-(2-Fluorobenzyl)piperazine Triazoles: A Novel Breast Cancer Cell Inhibitors

Beliyaiah,

Javarappa,

Dilkalal

et al. 2024

ajc

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New series of 1-(2-fluorobenzyl)piperazine triazoles 7(a-k) have been synthesized and evaluated for anticancer activity. The molecular structures of all the compounds were established by employing 1H NMR, 13C NMR and mass spectral analysis. In vitro anticancer activity was evaluated using MTT assay against MCF7 breast cancer cell line. Compounds 7i and 7j bearing 4-fluorophenyl and 2-fluorophenyl pendant from triazole substituent phenyl ring exhibited the highest anticancer efficacy with IC50 values of 12.09 µg/mL and 15.12 µg/mL, respectively. A molecular docking study conducted on human HER2 complexed with hercepatin fab was acquired from Protein Data Bank (PDB ID: 1N8Z). Molecular docking studies demonstrated Leu443, Gly442 and Leu27 as key residues interacting with active compounds.

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Section: Synthesis Anticancer Evaluation and Molecular Docking Studie...mentioning

confidence: 99%

Synthesis, Anticancer Evaluation and Molecular Docking Studies of 1-(2-Fluorobenzyl)piperazine Triazoles: A Novel Breast Cancer Cell Inhibitors

Beliyaiah,

Javarappa,

Dilkalal

et al. 2024

ajc

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“…While IRCFS can mitigate pests and disease ( Li et al., 2022 ; Yu et al., 2022 ), chemical control remains crucial to prevent losses in rice yield. As wind and drift often carry the droplets to non-target areas ( Hu et al., 2022 ), pesticide application raises environmental concerns since it would pose a potential risk to both the environment and crayfish.…”

Section: Introductionmentioning

confidence: 99%

Spray losses study of two pesticides by UASS in integrated rice–crayfish farming system and acute toxicity evaluation on Procambarus clarkii

Liu,

Wang,

et al. 2023

Front. Plant Sci.

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IntroductionWhile the integrated rice-crayfish (Procambarus clarkii) farming system (IRCFS) is widely developing in China, the widespread use of Unmanned Aerial Spraying Systems (UASS) to protect rice from pests has led to potential pesticide risk for the crayfish in IRCFS. Therefore, it is crucial to examine UASS’s spray deposition and drift in IRCFS.MethodIn this study, we used the oligonucleotide sequence-tracking / dot-blotting (OSTDB) method to trace pesticide spraying. We collected detailed data not only on spray loss in the paddy fields, but also on spray drift in the breeding ditches caused by upwind and downwind spray areas. Additionally, pesticide residues in the breeding ditches were measured using LC-MS/MS by collecting water samples after pesticide application.ResultsThe data analysis indicated that the spray loss in the paddy field was significantly greater than that in the breeding ditches. The spray drift in the breeding ditches, caused by the upwind spray area, was seven times higher than that originating from the downwind spray area. Furthermore, the results also revealed that the bulk flow between the paddy fields and the breeding ditches contributed a substantial amount of pesticide residue to the water body in the breeding ditches. In addition, we investigated the acute toxicities of common insecticides using in paddy fields, including thiamethoxam (THI), chlorantraniliprole (CHI), THI·CHI-Mix and THI·CHI-WG.DiscussionThe results demonstrated that the spray losses and spray drift from UASS spray applications of these pesticides in IRCFS would not cause acute toxicity or death in crayfish. These findings provide important materials for establishing pesticide application standards and guiding the field testing of droplet deposition and drift in IRCFS.

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“…The Chinese mitten crab, Eriocheir sinensis, is one of the most important aquaculture crustaceans in China [37]. So far, the roles of 20E and its receptors, i.e., EsEcR, EsRXR as well as EsMIH, in the regulation of molting have been studied in this species [22,[38][39][40]. It was found that EsEcR and EsRXR were highly expressed in the hepatopancreas at pre-molt and lowly expressed at post-molt, while EsMIH was highly expressed in eyestalk and showed the opposite expression pattern to that of EsEcR and EsRXR [40].…”

Section: Introductionmentioning

confidence: 99%

“…So far, the roles of 20E and its receptors, i.e., EsEcR, EsRXR as well as EsMIH, in the regulation of molting have been studied in this species [22,[38][39][40]. It was found that EsEcR and EsRXR were highly expressed in the hepatopancreas at pre-molt and lowly expressed at post-molt, while EsMIH was highly expressed in eyestalk and showed the opposite expression pattern to that of EsEcR and EsRXR [40]. In this study, a FOXOlike molecule containing an FH domain was identified and characterized in E. sinensis (designated as EsFOXO-like), with the objectives to (1) examine its expression pattern at three molting stages, (2) investigate the impact of EsFOXO-like on the concentration of 20E and the expression levels of genes related to molting (EsEcR, EsRXR and EsMIH) and (3) explore the involvement of mTOR in the regulation of EsFOXO-like and its impact on the 20E pathway.…”

Section: Introductionmentioning

confidence: 99%

FOXO-like Gene Is Involved in the Regulation of 20E Pathway through mTOR in Eriocheir sinensis

Yang

et al. 2023

JMSE

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The Forkhead Box O (FOXO) gene plays a key role in various biological processes, such as growth, metabolism, development, immunity and longevity. Molting is an essential process for crustacean growth, which is mainly regulated by 20-hydroxyecdysone (20E) and molt-inhibiting hormone (MIH). Although the role of FOXO in regulating the immune response of crustaceans is well documented, its involvement in controlling crustacean molting remains unclear. In this study, a FOXO-like gene (designed as EsFOXO-like) was identified in Eriocheir sinensis, and the regulation of the 20E pathway by EsFOXO-like was also investigated. The coding sequence of EsFOXO-like was 852 bp, which consisted of 283 amino acids including a conserved Forkhead (FH) domain. EsFOXO-like shared high similarity with FOXO genes from other crustaceans, and the mRNA expression levels of the EsFOXO-like gene were highest in the hepatopancreas and lowest in the hemocytes. However, transcription and protein expression of the EsFOXO-like gene were found to be up-regulated only during the pre-molt stage in the hepatopancreas, with lower expression levels observed at the post-molt stage. To explore the role of EsFOXO-like in the 20E pathway, EsFOXO-like was firstly inhibited by a specific FOXO inhibitor (AS1842856) and then through an EsFOXO-like dsRNA injection, respectively, and the results showed that the relative expression levels of EsFOXO-like were notably decreased in the hepatopancreas after both the inhibitor and dsRNA treatments. The 20E concentration, the mRNA expression levels of the 20E receptors including the ecdysone receptor (EcR) and the retinoid-X receptor (RXR) and EsmTOR transcription in the AS1842856 group or the EsFOXO-RNAi group were all significantly higher than that in the control group, while the mRNA expression level of EsMIH was significantly decreased after EsFOXO-like inhibition. To further investigate whether the EsFOXO-like acts through mTOR or not, Rapamycin was administered to inhibit mTOR activity in EsFOXO-like inhibited crabs. The results revealed a significant reduction in the concentration of 20E and the expression level of EsMIH in the AS1842856 + Rapamycin group compared to the AS1842856 + DMSO group, accompanied by an increase in EsEcR and EsRXR expression. These findings collectively suggest that EsFOXO-like regulates the 20E pathway through mTOR, which offered valuable insights into the understanding of the molting process in crustaceans.

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Effects of triazole plant growth regulators on molting mechanism in Chinese mitten crab (Eriocheir sinensis)

Cited by 4 publications

References 42 publications

Synthesis, Anticancer Evaluation and Molecular Docking Studies of 1-(2-Fluorobenzyl)piperazine Triazoles: A Novel Breast Cancer Cell Inhibitors

Synthesis, Anticancer Evaluation and Molecular Docking Studies of 1-(2-Fluorobenzyl)piperazine Triazoles: A Novel Breast Cancer Cell Inhibitors

Spray losses study of two pesticides by UASS in integrated rice–crayfish farming system and acute toxicity evaluation on Procambarus clarkii

FOXO-like Gene Is Involved in the Regulation of 20E Pathway through mTOR in Eriocheir sinensis

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