Effects of transport, fasting and anaesthesia on the faecal microbiota of healthy adult horses

Schoster, Angelika; Mosing, Martina; Jalali, Mohammad; Staempfli, H. R.; Weese, J. Scott

doi:10.1111/evj.12479

Cited by 62 publications

(60 citation statements)

References 35 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Collecting detailed information on each horse's recent diet and either matching cases and control horses based on diet or controlling for diet in statistical models would be important for future studies. Sampling time with respect to time of day, season, transportation and duration of time feed was withheld varied and may have influenced the data [44]. Despite these study limitations, the results support previous studies in horses and human patients whereby a decrease in bacterial richness and diversity is associated with GI disease.…”

Section: Discussionsupporting

confidence: 81%

Differences in the equine faecal microbiota between horses presenting to a tertiary referral hospital for colic compared with an elective surgical procedure

Stewart

Southwood

Indugu

et al. 2018

Equine Veterinary Journal

View full text Add to dashboard Cite

Changes in bacterial populations in the faecal microbiota of horses presenting for colic observed in this study concurs with previous studies in veterinary and human patients with gastrointestinal disease. Future studies focusing on different causes of colic, chronic or recurrent disease, and the association with histological changes within the intestine are needed. This article is protected by copyright. All rights reserved.

show abstract

Section: Discussionsupporting

confidence: 81%

Differences in the equine faecal microbiota between horses presenting to a tertiary referral hospital for colic compared with an elective surgical procedure

Stewart

Southwood

Indugu

et al. 2018

Equine Veterinary Journal

View full text Add to dashboard Cite

show abstract

“…These discrepancies can be explained by several possible factors including geographic location, breed, and husbandry of the horses, as well as methodological differences such as DNA extraction technique and sequencing platform. It is doubtful that this difference in sequencing platform accounts for the difference in detected phyla as one group has utilized both systems [29, 30, 33, 34] with samples collected from horses in a similar environment, and generated comparable results. Of note however, studies performed by Dougal et al [27, 28], using samples obtained from different groups of horses located in Michigan and the UK respectively, detected a Firmicutes to Bacteroidetes ratio similar to that reported here.…”

Section: Discussionmentioning

confidence: 99%

A Microbiological Map of the Healthy Equine Gastrointestinal Tract

et al. 2016

View full text Add to dashboard Cite

Horses are exquisitely sensitive to non-specific gastrointestinal disturbances as well as systemic and extraintestinal conditions related to gut health, yet minimal data are available regarding the composition of the microbiota present in the equine stomach, small intestine, and cecum and their relation to fecal microbiota. Moreover, there is minimal information regarding the concordance of the luminal and mucosal microbial communities throughout the equine gut. Illumina-based 16S rRNA gene amplicon sequencing of the luminal and mucosal microbiota present in seven regions of the gastrointestinal tract of nine healthy adult horses revealed a distinct compositional divide between the small and large intestines. This disparity in composition was more pronounced within the luminal contents, but was also detected within mucosal populations. Moreover, the uniformity of the gut microbiota was much higher in the cecum and colon relative to that in the stomach, jejunum and ileum, despite a significantly higher number of unique sequences detected in the colon. Collectively, the current data suggest that while colonic samples (a proxy for feces) may provide a reasonable profile of the luminal contents of the healthy equine large intestine, they are not informative with regard to the contents of the stomach or small intestine. In contrast to the distinct difference between the highly variable upper gastrointestinal tract microbiota and relatively uniform large bowel microbiota present within the lumen, these data also demonstrate a regional continuity present in mucosal microbial communities throughout the length of the equine gut.

show abstract

“…14 Transportation is associated with factors that may contribute to gastric ulceration such as physiologic stress responses (increased heart rate and serum cortisol concentrations), [15][16][17][18][19][20][21] changes in feeding practices and water consumption, 22,23 and changes in GI microbiota. 12,24 Our study was conducted to determine the impact of 12 hours of transportation without food or water on gastric ulcer scores and gastric fluid (GF) pH in horses. A secondary objective was to determine the effect of pretransport feeding practices on these outcomes.…”

Section: Introductionmentioning

confidence: 99%

Effects of transportation on gastric pH and gastric ulceration in mares

Padalino

Davis

Raidal

2020

Veterinary Internal Medicne

View full text Add to dashboard Cite

Background Transportation has been suggested as a risk factor for gastric ulceration in horses, but limited evidence supports this assumption. Animals Twenty‐six Standardbred, Thoroughbred, and Warmblood mares from a university teaching herd. Methods Twelve mares were confined for 12 hours, overnight, in reproductive stocks with indwelling nasogastric tubes (NGTs) to assess pH of gastric fluid (GF). Gastric ulceration was assessed endoscopically before and after confinement. Subsequently, 26 horses were transported for 12 hours, overnight, in 2 consignments. During transportation, GF was aspirated from indwelling NGT placed in the same 12 mares used in the confinement study, and gastric ulceration was assessed endoscopically before and after transportation in all horses. Results The median pH of GF in confined horses was 1.70‐2.49 at each sampling point, and there was no apparent effect on gastric squamous ulcer scores. The median pH of GF from the same 12 horses at corresponding sampling times during transportation was 6.82‐7.22. Transportation was associated with increased gastric squamous ulcer scores, particularly in horses fasted for gastroscopy and NGT placement immediately before departure. Gastric emptying appeared delayed after transportation in horses fed before departure. Conclusions and Clinical Importance Transportation is associated with increased gastric squamous ulceration and with increased pH of GF. These findings may be a consequence of impaired gastric emptying and reflux of alkaline small intestinal content, with factors such as duodenal bile salts and short‐chain fatty acids mediating mucosal injury.

show abstract

Effects of transport, fasting and anaesthesia on the faecal microbiota of healthy adult horses

Cited by 62 publications

References 35 publications

Differences in the equine faecal microbiota between horses presenting to a tertiary referral hospital for colic compared with an elective surgical procedure

Differences in the equine faecal microbiota between horses presenting to a tertiary referral hospital for colic compared with an elective surgical procedure

A Microbiological Map of the Healthy Equine Gastrointestinal Tract

Effects of transportation on gastric pH and gastric ulceration in mares

Contact Info

Product

Resources

About