Effects of transforming growth factor‐β1 on plasminogen activation in stem cells from the apical papilla: role of activating receptor‐like kinase 5/Smad2 and mitogen‐activated protein kinase kinase (MEK)/extracellular signal‐regulated kinase (ERK) signalling

Chang, Mei‐Chi; Chang, Hung‐Hao; Hsieh, Wen-Shyong; Huang, Weiling; Lian, Yun‐Chia; Jeng, Po-Yuan; Wang, Yin‐Lin; Yeung, Sin‐Yuet; Jeng, Jiiang‐Huei

doi:10.1111/iej.13266

Cited by 9 publications

(9 citation statements)

References 66 publications

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“…The higher concentrations of TGF‐β1 (5 and 10 ng mL −1 ) generated significantly more deteriorating effects to cells than the lower concentrations. The findings correlate with a previous study showing that SCAP cultured in the presence of TGF‐β1 had less cell viability, and the use of high concentration (10 ng mL −1 ) generated the negative effect (19). Similar to earlier studies that reported the cytostatic effect of TGF‐β1 on various mammalian cells via various pathways, including the suppression of several key transcription factors regulating growth and the expression of cyclin‐dependent kinase (CDK) inhibitor (24,25).…”

Section: Discussionsupporting

confidence: 90%

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Direct effect of transforming growth factor‐beta 1 (TGF‐β1) on human apical papilla cell proliferation and mineralisation

Srisuwan

Wattanapakkavong²

2021

Aust Endodontic J

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Firstly, this study investigated the direct effect of Transforming Growth Factorbeta 1 at 10, 5, 2.5 and 1.25 ng mL À1 on human apical papilla cell proliferation and mineralisation. Cell proliferation was examined at 0, 2, 4, 6, 24, 48, 72, 96 and 120 h using Alamar Blue TM assay. Cell mineralisation was examined at day 21 with a quantitative Alizarin Red S staining. Secondly, the study aimed to estimate the amount of Transforming Growth Factor-beta 1 (TGF-b1) released from the dentin after root canal irrigation. The solution collected from a root canal after rinsing with various protocols (normal saline solution, ethylenediaminetetraacetic acid or chlorhexidine) was analysed with an enzyme-linked immunosorbent assay. Data were statistically analysed using a one-way analysis of variance. The results, from the first part, revealed cell proliferation reduction in all experimental groups presented with TGF-b1. The higher concentration generated more deteriorating effects. Cell mineralisation was highest in a group with TGF-b1 at 1.25 ng mL À1 (P < 0.05). For the growth factor released from dentin, the highest amount was detected only when ethylenediaminetetraacetic acid was associated with the irrigation (P < 0.05). In summary, the direct effects of TGF-b1 on cell proliferation and differentiation were diverse, depending on concentration. The release of TGF-b1 from root dentin can be achieved after rinsing with ethylenediaminetetraacetic acid.

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Section: Discussionsupporting

confidence: 90%

“…Promising results were consistently reported showing cell viability improvement and cell differentiation (17,18). Moreover, a recent study proposed that TGF‐β1 might affect the repair/regeneration activities of stem cells from apical papilla (SCAP) via ALK5/Smad2/3 and MEK/ERK activation (19).…”

Section: Introductionmentioning

confidence: 99%

Direct effect of transforming growth factor‐beta 1 (TGF‐β1) on human apical papilla cell proliferation and mineralisation

Srisuwan

Wattanapakkavong²

2021

Aust Endodontic J

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“…81 Chang et al showed that TGF-b stimulates PAI-1 and suPAR secretion in a system of repair/regeneration activities of stem cells from apical papilla (SCAP). 82 Besides a widespread thrombosis in patients with COVID-19, the examination of the lung during an autopsy of 7 deceased COVID-19 patients also demonstrated increased angiogenesis. 76 Recent evidence has revealed higher vascular endothelial growth factor (VEGF) levels in COVID-19 patients than in healthy controls.…”

Section: Angiogenesis and Coagulationmentioning

confidence: 96%

“…Chang et al . showed that TGF‐β stimulates PAI‐1 and suPAR secretion in a system of repair/regeneration activities of stem cells from apical papilla (SCAP) 82 …”

Section: Involvement Of Tgf‐β In Different Pathogenesesmentioning

confidence: 99%

RGD‐binding integrins and TGF‐β in SARS‐CoV‐2 infections – novel targets to treat COVID‐19 patients?

Carvacho

Piesche

2021

Clin & Trans Imm

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The new coronavirus SARS-CoV-2 is a global pandemic and a severe public health crisis. SARS-CoV-2 is highly contagious and shows high mortality rates, especially in elderly and patients with pre-existing medical conditions. At the current stage, no effective drugs are available to treat these patients. In this review, we analyse the rationale of targeting RGD-binding integrins to potentially inhibit viral cell infection and to block TGF-b activation, which is involved in the severity of several human pathologies, including the complications of severe COVID-19 cases. Furthermore, we demonstrate the correlation between ACE2 and TGF-b expression and the possible consequences for severe COVID-19 infections. Finally, we list approved drugs or drugs in clinical trials for other diseases that also target the RGD-binding integrins or TGF-b. These drugs have already shown a good safety profile and, therefore, can be faster brought into a trial to treat COVID-19 patients.

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“…SCAPs can differentiate into radicular pulp as well as the odontoblasts that are responsible for root dentinogenesis, and undergo morphological and functional changes. These postnatal stem cells play a crucial role in pulp-dentine complex healing and regeneration when the roots of young permanent teeth are damaged (Chang et al, 2020). A greater understanding of the cellular and molecular mechanisms that regulate human SCAPs (hSCAPs) activity is likely to improve tooth tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Hsa‐miRNA‐143‐3p regulates the odontogenic differentiation of human stem cells from the apical papilla by targeting NFIC

Gao

Zhao

et al. 2021

Int Endodontic J

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Aim: To evaluate the effects of hsa-miRNA-143-3p on the cytodifferentiation of human stem cells from the apical papilla (hSCAPs) and the post-transcriptional regulation of Nuclear factor I-C (NFIC).Methodology: miRNA expression profiles in human immature permanent teeth and during hSCAP differentiation were examined. hSCAPs were treated with miR-143-3p overexpression or silencing viruses, and the proliferation and odontogenic and osteogenic differentiation of these stem cells, and the involvement of the NFIC pathway, were investigated. Luciferase reporter and NFIC mutant plasmids were used to confirm NFIC mRNA as a direct target of miR-143-3p. NFIC expression analysis in the miR-143-3p overexpressing hSCAPs was used to investigate whether miR-143-3p functioned by targeting NFIC. Student's t-test and chi-square tests were used for statistical analysis.Results: miR-143-3p expression was screened by microarray profiling and was found to be significantly reduced during hSCAP differentiation (p < .05). Overexpression of miR-143-3p inhibited the mineralization of hSCAPs significantly (p < .05) and downregulated the levels of odontogenic differentiation markers (NFIC [p < .05], DSP [p < .01] and KLF4 [p < .01]), whereas silencing of miR-143-3p had the opposite effect. The luciferase reporter gene detection and bioinformatic approaches identified NFIC mRNA as a potential target of miR-143-3p. NFIC overexpression reversed the inhibitory effect of miR-143-3p on the odontogenic differentiation of hSCAPs.Conclusions: miR-143-3p maintained the stemness of hSCAPs and modulated their differentiation negatively by directly targeting NFIC. Thus, inhibition of this miRNA represents a potential strategy to promote the regeneration of damaged tooth roots.

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Effects of transforming growth factor‐β1 on plasminogen activation in stem cells from the apical papilla: role of activating receptor‐like kinase 5/Smad2 and mitogen‐activated protein kinase kinase (MEK)/extracellular signal‐regulated kinase (ERK) signalling

Abstract: of transforming growth factor-b1 on plasminogen activation in stem cells from the apical papilla: role of activating receptorlike kinase 5/Smad2 and mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signalling.

Cited by 9 publications

References 66 publications

Direct effect of transforming growth factor‐beta 1 (TGF‐β1) on human apical papilla cell proliferation and mineralisation

Direct effect of transforming growth factor‐beta 1 (TGF‐β1) on human apical papilla cell proliferation and mineralisation

RGD‐binding integrins and TGF‐β in SARS‐CoV‐2 infections – novel targets to treat COVID‐19 patients?

Hsa‐miRNA‐143‐3p regulates the odontogenic differentiation of human stem cells from the apical papilla by targeting NFIC

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