Effects of transforming growth factor-β1 and vascular endothelial growth factor 165 gene transfer on Achilles tendon healing

Hou, Yu; Mao, Zebin; Wei, Xuelei; Lin, Lin; Chen, Lianxu; Wang, Haijun; Fu, Xin; Zhang, JiYing; Yu, Chunxiao

doi:10.1016/j.matbio.2009.04.007

Cited by 87 publications

(66 citation statements)

References 75 publications

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“…When TGF is added to the healing tendon, it has been shown to induce the expression of collagen I and III and also improve the mechanical properties of the tendon, seemingly accelerating the healing process (Hou et al, 2009;Kashiwagi et al, 2004). It may be that a single exposure to TGF-β in sufficient dosage is already enough to mobilize and differentiate additional mesenchymal stem cells (Kashiwagi et al, 2004).…”

Section: Tgf-βmentioning

confidence: 99%

Tendon Healing with Growth Factors

Müller¹,

Todorov²,

Heisterbach³

et al. 2012

Achilles Tendon

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Section: Tgf-βmentioning

confidence: 99%

Tendon Healing with Growth Factors

Müller¹,

Todorov²,

Heisterbach³

et al. 2012

Achilles Tendon

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“…The family of vascular endothelial growth factor (VEGF) molecules are potent initiators and regulators of angiogenesis with VEGF 165 being the most powerful and widely distributed within the body for promoting angiogenesis (Neufeld et al 1999;Zhao et al 2007). BMMSCs transfected with the VEGF gene have been used in the treatment of cardiovascular disease (Liu et al 2009;Pons et al 2009;Zisa et al 2009), bone regeneration , neurodegenerative , and Achilles tendon repair (Hou et al 2009). However, there has not been research on VEGF gene modified BMMSCs and its role in the liver disease process.…”

Section: Introductionmentioning

confidence: 99%

VEGF165 expressing bone marrow mesenchymal stem cells differentiate into hepatocytes under HGF and EGF induction in vitro

Yan

Xiao

et al. 2012

Cytotechnology

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A short half-life and low levels of growth factors in an injured microenvironment necessitates the sustainable delivery of growth factors and stem cells to augment the regeneration of injured tissues. Our aim was to investigate the ability of VEGF 165 expressing bone marrow mesenchymal stem cells (BMMSCs) to differentiate into hepatocytes when cultured with hepatocyte growth factor (HGF) and epidermal growth factor (EGF) in vitro. We isolated, cultured and identified rabbit BMMSCs, then electroporated the BMMSCs with VEGF 165 -pCMV6-AC-GFP plasmid. G418 was used to select transfected cells and the efficiency was up to 70%. The groups were then divided as follows: Group A was electroporated with pCMV6-AC-GFP plasmid ? HGF ? EGF and Group B was electroporated with VEGF 165 -pCMV6-AC-GFP plasmid ?HGF ? EGF. After 14 days, BMMSCs were induced into short spindle and polygonal cells. Alpha-fetoprotein (AFP) was positive and albumin (ALB) was negative in Group A, while both AFP and ALB were positive in group B on day 10. AFP and ALB in both groups were positive on day 20, but the quantity of AFP in group B decreased with prolonged time and was about 43.5% less than group A. The quantity of the ALB gene was increased with prolonged time in both groups. However, there was no significant difference between group A and B on day 10 and 20. Our results demonstrated that VEGF 165 -pCMV6-AC-GFP plasmid modified BMMSCs still had the ability to differentiate into hepatocytes. The VEGF 165 gene promoted BMMSCs to differentiate into hepatocyte-like cells under the induction of HGF and EGF, and reduced the differentiation time. These results have implications for cell therapies.

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“…Although TGF-β1 stimulation induces the proliferation of PDL cells (Brady et al 1998;Okubo et al 2003) and the expression of ECM components in PDL cells (Worapamorn et al 2001;Fujita et al 2004), the effects of TGF-β1 on differentiation and fiber production of PDL cells in the process of PDL regeneration are not fully understood. Hou et al (2009) have reported that TGF-β1 gene transfer into bone-marrow-derived mesenchymal stem cells accelerates the regeneration of ligament/tendon defects through the upregulation of Col I and type III collagen. Therefore, we have hypothesized that TGF-β1 might be important in the proliferation and differentiation of PDL cells for the induction of the fiber synthesis necessary to regenerate PDL tissues.…”

Section: Introductionmentioning

confidence: 99%

Effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line

et al. 2010

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Periodontal ligament (PDL) is a specialized connective tissue that influences the lifespan of the tooth. Transforming growth factor-β1 (TGF-β1) is a multifunctional cytokine, but little is known about the effects of TGF-β1 on PDL cells. Our aim has been to demonstrate the expression of TGF-β1 in rat PDL tissues and to evaluate its effects on the proliferation and gene expression in human PDL cells (HPLCs) and a human PDL stem/progenitor cell line, line 1-11, that we have recently developed. The expression of TGF-β1 in the entire PDL tissue was confirmed immunohistochemically, and both HPLCs and cell line 1-11 expressed mRNA from the TGF-β1, TGF-β type I receptor, and TGF-β type II receptor genes. Although exogenous TGF-β1 stimulated the proliferation of HPLCs, it did not upregulate the expression of alpha-smooth muscle actin (α-SMA), type I collagen (Col I), or fibrillin-1 (FBN1) mRNA or of α-SMA protein in HPLCs, whereas expression for these genes was attenuated by an anti-TGF-β1 neutralizing antibody. In contrast, exogenous TGF-β1 reduced the proliferation of cell line 1-11, although it upregulated the expression of α-SMA, Col I, and FBN1 mRNA and of α-SMA protein in this cell line. In addition, interleukin-1 beta stimulation significantly reduced the expression of TGF-β1 mRNA and protein in HPLCs. Thus, TGF-β1 seems to play an important role in inducing fibroblastic differentiation of PDL stem/progenitor cells and in maintaining the PDL apparatus under physiological conditions.

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Effects of transforming growth factor-β1 and vascular endothelial growth factor 165 gene transfer on Achilles tendon healing

Cited by 87 publications

References 75 publications

Tendon Healing with Growth Factors

Tendon Healing with Growth Factors

VEGF165 expressing bone marrow mesenchymal stem cells differentiate into hepatocytes under HGF and EGF induction in vitro

Effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line

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