Effects of trans-10, cis-12 conjugated linoleic acid on body fat and serum lipids in young and adult hamsters

Navarro, Virginia; Miranda, Jonatan; Churruca, Itziar; Fernández‐Quintela, Alfredo; Rodríguez, Víctor M.; Portillo, Marı́a P.

doi:10.1007/bf03174069

Cited by 16 publications

(9 citation statements)

References 32 publications

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“…SCHOENHERR and JEWELL (51) found that dietary CLA failed to reduce body fat in adult beagles. As shown in another paper in this issue, results from our laboratory reveal that 0.5% t-10, c-12 CLA in a highfat diet significantly reduced adipose tissue size in both young (9-week-old) and adult (8-month-old) hamsters, but the percentage of reduction for a pool of perirenal, epididymal and subcutaneous depots was greater in young than in adult hamsters (37).…”

Section: Agementioning

confidence: 70%

The body fat-lowering effect of conjugated linoleic acid: a comparison between animal and human studies

Navarro

Fernández‐Quintela

Churruca

et al. 2006

J. Physiol. Biochem.

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Different reasons which justify differences between rodents and humans in body fat reduction produced by conjugated linoleic acid (CLA) could be proposed. The doses used in humans are lower than those used in rodents. Human experiments have been performed with CLA isomer mixtures instead of isolated isomers. The variable dilution of t-10, c-12, the active isomer, among different preparations might explain the reduced responsiveness in humans. Diet composition may modulate CLA effects on body fat accumulation. As far as human studies are concerned, a specific dietary pattern has not been established. As a result differences among studies and also among subjects in the same study are likely. In rodents, the effects of CLA vary with genotype, suggesting that genetic predisposition to fat accumulation can play an important role in the effectiveness of CLA. Human volunteers with different body mass index have participated in the published studies and even in the same experiment. So, differences in lipid metabolism among subjects could help to explain the discrepancies observed in the literature. Age and maturity may also be crucial. Experiments using rodents have been conducted with growing animals and there is little evidence of CLA effectiveness in adult animals. By contrast, human studies have been performed with adults. Inhibition of lipogenesis in white adipose tissue is one of the mechanisms which have been proposed to explain the body-fat lowering effect of CLA, but lipogenesis in this tissue is very low in humans. Another mechanism suggested is increased fatty acid oxidation in the liver associated with peroxisome proliferation, but humans are relatively insensitive to this effect.

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Section: Agementioning

confidence: 70%

The body fat-lowering effect of conjugated linoleic acid: a comparison between animal and human studies

Navarro

Fernández‐Quintela

Churruca

et al. 2006

J. Physiol. Biochem.

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“…These results are not in good accordance with those reported by STANGL (26), who observed increased body fat loss in rats fed a 30% energy-restricted diet, supplemented with 3% of a CLA mixture (0.55% trans-10,cis-12 CLA) when they were compared with control restricted rats. This discrepancy may be due to differences in some aspects of the experimental design, such as the species, their age and the lipid source, which have been shown to have an important influence on CLA effects (14,16,17,19,27). While Stangl (26) used rats, the present work was carried out with hamsters.…”

Section: Discussionmentioning

confidence: 99%

Adiposity and serum parameters in hamsters fed energy restricted diets supplemented or not with trans-10,cis-12 conjugated linoleic acid

Lasa

Simón

Churruca

et al. 2007

J. Physiol. Biochem.

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Numerous studies have demonstrated that conjugated linoleic acid (CLA) modulates body composition, reducing body fat accumulation in various mammalian species. However, very few studies have been carried out to assess the effect of CLA on previously stored body fat. The aim of the present work was to analyse the effectiveness of trans-10,cis-12 CLA in improving alterations produced by high-fat feeding in body fat and serum parameters when it was included in an energy-restricted diet. For this purpose male Syrian Golden hamsters were fed on high-fat diet for 7 weeks in order to increase their body fat content, and a further 25% energy-restricted diet supplemented or not with 0.5% trans-10,cis-12 CLA for 3 weeks. Adipose tissues, liver and gastrocnemious muscles were dissected and weighed. Adipocyte diameter and number were assessed in epididymal adipose tissue. Total cholesterol, triacylglycerols, non-esterified fatty acids and glucose were measured in serum. Three weeks of energy restriction resulted in a reduction in body weight and white adipose tissue size in all anatomical locations, without changes in liver and gastrocnemious muscle weights. Epididymal adipocyte size was reduced, but total adipocyte number remained unchanged. Serum cholesterol, triacylglycerols and glucose were significantly reduced. No differences were observed between the restricted groups (control and CLA supplemented). In conclusion, under our experimental conditions, the addition of trans-10,cis-12 CLA to the diet does not increase the benefits produced by energy restriction.

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“…It has been reported that t10, c12 CLA is the most potent isomer in terms of potential to prevent cell proliferation and induce apoptosis in cancer cells (Cho et al, 2005(Cho et al, , 2006Kim et al, 2002a; Lee et al, 2006b; Ochoa et al, 2004). t10, c12 CLA is also associated with decreased body fat and increased lean body mass in various animal species, such as mice (Park et al, 1997), chickens (Cherian et al, 2005), rats (Yamasaki et al, 2003), hamsters (Navarro et al, 2006), pigs (Ostrowska et al, 1999) and, to some extent, humans (Blankson et al, 2000;Mougios et al, 2001; Risérus et al, 2001;Smedman & Vessby, 2001;Thom et al, 2001). Commensal Bifidobacterium and Lactobacillus species from the mammalian gastrointestinal Abbreviations: CLA, conjugated linoleic acid; FAME, fatty acid methyl ester; PAI, Propionibacterium acnes isomerase.…”

Section: Introductionmentioning

confidence: 99%

Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice

Rosberg-Cody

Stanton

O’Mahony³

et al. 2011

Microbiology

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We have previously demonstrated that oral administration of a metabolically active Bifidobacterium breve strain, with ability to form cis-9, trans-11 conjugated linoleic acid (CLA), resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 CLA and omega-3 (n-3) fatty acids in liver and adipose tissue. In this study, we investigated whether a recombinant lactobacillus expressing linoleic acid isomerase (responsible for production of t10, c12 CLA) from Propionibacterium acnes (PAI) could influence the fatty acid composition of different tissues in a mouse model. Linoleic-acid-supplemented diets (2 %, w/w) were fed in combination with either a recombinant t10, c12 CLA-producing Lactobacillus paracasei NFBC 338 (Lb338), or an isogenic (vector-containing) control strain, to BALB/c mice for 8 weeks. A third group of mice received linoleic acid alone (2 %, w/w). Tissue fatty acid composition was assessed by GLC at the end of the trial. Ingestion of the strain expressing linoleic acid isomerase was associated with a 4-fold increase (P,0.001) in t10, c12 CLA in adipose tissues of the mice when compared with mice that received the isogenic non-CLA-producing strain. The livers of the mice that received the recombinant CLA-producing Lb338 also contained a 2.5-fold (albeit not significantly) higher concentration of t10, c12 CLA, compared to the control group. These data demonstrate that a single gene (encoding linoleic acid isomerase) expressed in an intestinal microbe can influence the fatty acid composition of host fat. INTRODUCTIONEvidence is emerging to support the concept that the enteric microbiota can exert profound effects on human health and disease, involving complex host-bacteria interactions that are as yet poorly understood. The gut microbiota is important to the host with regard to metabolic functions, providing nutrients and conferring an ability to resist bacterial infections (Marchesi & Shanahan, 2007;Wilks, 2007), as well as playing a dominant role in the education of the intestinal mucosal immune responses. Furthermore, the enteric microbiota has been shown to exert effects on disease processes outside the gut, including an impact on obesity and non-alcoholic fatty liver disease (Dumas et al., 2006;Marchesi & Shanahan, 2007).Conjugated linoleic acid (CLA) is a beneficial bacterial metabolite formed via microbial isomerization of linoleic acid. CLA is a collective term describing different isomers of linoleic acid with conjugated double bonds, the cis-9, trans-11 (c9, t11) CLA isomer being the most abundant form and the t10, c12 CLA isomer accounting for~1 % of total milk fat CLA (Jensen, 2002). It has been reported that t10, c12 CLA is the most potent isomer in terms of potential to prevent cell proliferation and induce apoptosis in cancer cells (Cho et al., 2005(Cho et al., , 2006Kim et al., 2002a; Lee et al., 2006b; Ochoa et al., 2004). t10, c12 CLA is also associated with decreased body fat and increased lean body mass in various animal...

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Effects of trans-10, cis-12 conjugated linoleic acid on body fat and serum lipids in young and adult hamsters

Cited by 16 publications

References 32 publications

The body fat-lowering effect of conjugated linoleic acid: a comparison between animal and human studies

The body fat-lowering effect of conjugated linoleic acid: a comparison between animal and human studies

Adiposity and serum parameters in hamsters fed energy restricted diets supplemented or not with trans-10,cis-12 conjugated linoleic acid

Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice

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