Adiposity and serum parameters in hamsters fed energy restricted diets supplemented or not with trans-10,cis-12 conjugated linoleic acid

Abstract

Numerous studies have demonstrated that conjugated linoleic acid (CLA) modulates body composition, reducing body fat accumulation in various mammalian species. However, very few studies have been carried out to assess the effect of CLA on previously stored body fat. The aim of the present work was to analyse the effectiveness of trans-10,cis-12 CLA in improving alterations produced by high-fat feeding in body fat and serum parameters when it was included in an energy-restricted diet. For this purpose male Syrian Golden hamsters were fed on high-fat diet for 7 weeks in order to increase their body fat content, and a further 25% energy-restricted diet supplemented or not with 0.5% trans-10,cis-12 CLA for 3 weeks. Adipose tissues, liver and gastrocnemious muscles were dissected and weighed. Adipocyte diameter and number were assessed in epididymal adipose tissue. Total cholesterol, triacylglycerols, non-esterified fatty acids and glucose were measured in serum. Three weeks of energy restriction resulted in a reduction in body weight and white adipose tissue size in all anatomical locations, without changes in liver and gastrocnemious muscle weights. Epididymal adipocyte size was reduced, but total adipocyte number remained unchanged. Serum cholesterol, triacylglycerols and glucose were significantly reduced. No differences were observed between the restricted groups (control and CLA supplemented). In conclusion, under our experimental conditions, the addition of trans-10,cis-12 CLA to the diet does not increase the benefits produced by energy restriction.

The effect of periodic energy restriction on growth performance, serum biochemical indices, and meat quality in sheep

“…Limiting energy intake resulted in lambs fed the CON diet having (P < 0.05) greater ADG compared with lambs fed the RES diet (158.79 vs. 57.01 g/d, for CON vs. RES, respectively). Energy restriction resulting in the decreased ADG is similar to previous research (Lasa et al, 2007;Tripathi et al, 2007). Per the study design, ADG were (P < 0.05) greater for lambs fed CON diet compared with lambs fed RES diet at the end of each phase.…”

“…Physiologically, serum TG roles are to maintain the dynamic lipoprotein balance for tissue repair, maintain blood pH, and as necessary provide energy via oxidation (Steers, 2016). Moreover, Lasa et al (2007) reported that serum CHO, TG, and GLU were significantly reduced during energy restriction, in which GLU was negatively and TG was positively correlated with Leptin, which plays a key role in the regulation of metabolism to regulate energy homeostasis, neuroendocrine, and metabolic functions (Singhal et al, 2002;Vijay et al, 2009;Dardeno et al, 2010). On the contrary, some researchers have reported that serum LDL, HDL, and TG were not significantly affected by feeding a high-or low-fat diet (Egret et al, 2011), which may be related to the severity and duration of the ME restriction and BW (Critser et al, 1995).…”

Conjugated nonadecadienoic acid is more potent than conjugated linoleic acid on body fat reduction

“…For example, both CNA and CLA have been reported to reduce fat uptake by inhibiting lipoprotein lipase activity in 3T3-L1 adipocytes, where CNA had similar inhibitory effect on lipoprotein lipase activity compared to CLA [6,10,19]. Effects of CLA on HSL have not been consistent; expression increased in 3T3-L1 adipocytes, decreased in human adipose tissue, or not changed in hamster adipose tissue [22][23][24]. In this report, CNA, but not CLA treatment as low as 0.01% of diet, showed significant increased HSL expression in white adipose tissue, reflecting increased lipolysis (Fig.…”

Effect of Wakame and Carob Pod Snacks on Non-Alcoholic Fatty Liver Disease

“…WC diet represented the standard normal-caloric diet for the treatment of metabolic syndrome in human. It was prepared according to previous studies from our laboratory with rodents [10], as well as to recommendations of the American Institute of Nutrition (AIN-93M) [11]. Considering that oat flour was present in snack formulation, starch for OC diet came from oat and wheat flour in similar amounts.…”