Effects of toxicants with different modes of action on Myriophyllum spicatum in test systems with varying complexity

Mohr, Silvia; Schott, Johanna; Maletzki, Dirk; Hünken, Andreas

doi:10.1016/j.ecoenv.2013.06.030

Cited by 12 publications

(17 citation statements)

References 33 publications

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“…Growth rate (RGR-L and RGR-DW) reductions in M. spicatum with increasing concentrations of IPU are not surprising given that this herbicide inhibits the electron transport chain in photosystem II (Bi et al, 2012). Our EC 50 values are within the range of those found by Mohr et al (2013) in a similar sediment-free test system after 14 d of exposure. However, contrary to this publication, we observed that shoot length decreased.…”

Section: Discussionsupporting

confidence: 76%

“…Sucrose enhanced the tolerance of Arabidopsis thaliana to atrazine and other photosystem-targeted herbicides like simazine, diuron and isoproturon (Sulmon et al, 2007(Sulmon et al, , 2004. Thus, the ten-18 fold higher sucrose concentration used by Mohr et al (2013) might have mitigated IPU effects on their plants, and impeded shoot growth reduction.…”

Section: Discussionmentioning

confidence: 95%

“…We used only 0.3% of sucrose, as this was sufficient to allow good plant growth (AN & EMG, unpublished results). In contrast, Mohr et al (2013) used Andrews medium with 3% sucrose. Sucrose enhanced the tolerance of Arabidopsis thaliana to atrazine and other photosystem-targeted herbicides like simazine, diuron and isoproturon (Sulmon et al, 2007(Sulmon et al, , 2004.…”

Section: Discussionmentioning

confidence: 97%

“…For each treatment combination (nitrate level x herbicide concentration), we used three flasks as individual replicates. Herbicide concentration-response curves were performed with concentrations of 0, 0.05, 0.5, 5, 50, 500, 1000 µg L -1 IPU or MSM, as these concentrations include published EC50 values based on growth for IPU and metsulfuronmethyl, another sulfonylurea herbicide (Cedergreen et al 2004;Mohr et al 2013). Each flask contained four apices.…”

Section: Ii2 Experimentsmentioning

confidence: 99%

“…IPU blocks electron transport in photosystem II (Pérès et al, 1996), and leads to oxidative stress (Rutherford and Krieger-Liszkay, 2001). IPU negatively affects many aquatic primary producers, with EC 50 values of 43.25 µg L -1 for Chlamydomonas reinhardtii, of 60 µg L -1 for Lemna minor, and between 22 and 53 µg L -1 , depending on the endpoint considered, for Myriophyllum spicatum (Bi et al, 2012;Mohr et al, 2013;Nitschke et al, 1999). MSM…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Does nitrate co-pollution affect biological responses of an aquatic plant to two common herbicides?

Nuttens¹,

Chatellier

Devin

et al. 2016

Aquatic Toxicology

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 97%

Section: Ii2 Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Does nitrate co-pollution affect biological responses of an aquatic plant to two common herbicides?

Nuttens¹,

Chatellier

Devin

et al. 2016

Aquatic Toxicology

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Some arguments in favor of a Myriophyllum aquaticum growth inhibition test in a water–sediment system as an additional test in risk assessment of herbicides

Tunić

Knežević

Kerkez

et al. 2015

Enviro Toxic and Chemistry

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The present study compares the practicability, reproducibility, power, and sensitivity of a Myriophyllum aquaticum growth inhibition test in a water-sediment system with the recently accepted Myriophyllum spicatum test in an equivalent testing system and the standard Lemna sp. test. Special consideration was given to endpoints based on M. aquaticum control plant growth and variability of relative growth rate and yield: shoot length, fresh weight, dry weight, and root weight. Sensitivity analysis was based on tests performed with 3,5-dichlorophenol, atrazine, isoproturon, trifluralin, 2,4-dichlorophenoloxyacetic acid, and dicamba. Growth rates for average M. aquaticum control plants were 0.119 d(-1) and 0.112 d(-1), with average estimated doubling time 6.33 d and 6.74 d for relative growth rate fresh weight and shoot length, respectively. Intrinsic variability of M. aquaticum endpoints was low: 12.9%, 12.5%, and 17.8% for relative growth rate shoot length, relative growth rate fresh weight and yield fresh weight, respectively. The power of the test was fairly high. When the most sensitive endpoints were used for comparison, the 2 Myriophyllum species were similarly sensitive, more sensitive (in the case of auxin simulators), or at least equally sensitive as Lemna minor to other tested herbicides. The M. aquaticum 10-d test with a 7-d exposure period in a water-sediment system has acceptable sensitivity and can provide repeatable, reliable, and reproducible results; therefore, it should not be disregarded as a good and representative additional test in environmental risk assessment.

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Sucrose modifies growth and physiology in axenically grown Myriophyllum spicatum with potential effects on the response to pollutants

Nuttens

Gross

2016

Enviro Toxic and Chemistry

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Sucrose as a carbon source in axenic tests affects plant growth and physiology. The high sucrose concentration in Organisation for Economic Co-operation and Development (OECD) guideline 238 for the submerged growing aquatic plant Myriophyllum spicatum might modify pollutant effects, thus impairing environmental risk assessment. In a factorial design experiment with axenic M. spicatum exposed to 3 sucrose concentrations (no, low, and high) with or without cadmium, growth, dry matter content, content in pigments or phenolic compounds, and elemental stoichiometry of carbon (C), nitrogen (N), and phosphorus (P) were measured. The results show that sucrose is crucial for growth but can be used at lower concentrations than currently considered. Sucrose-treated plants had higher dry matter content and C content but lower contents of chlorophyll and N. Cadmium affected the content in chlorophyll, phenolic compounds, and elemental stoichiometry. Interactive effects were observed on length growth, C and N content, and the C:N and N:P molar ratios. Remarkably, cadmium led to increased shoot length at low, but not at high, sucrose concentration. This contrasting effect might result from differences in osmotic potential caused by sucrose. Overall, the results suggest a strong effect of sucrose concentration on the growth and physiology of M. spicatum and modifications of the response to cadmium. Further studies should establish the lowest sucrose level needed to account for realistic environmental risk assessment based on the axenic OECD 238. Environ Toxicol Chem 2017;36:969-975. © 2016 SETAC.

show abstract

Effects of toxicants with different modes of action on Myriophyllum spicatum in test systems with varying complexity

Cited by 12 publications

References 33 publications

Does nitrate co-pollution affect biological responses of an aquatic plant to two common herbicides?

Does nitrate co-pollution affect biological responses of an aquatic plant to two common herbicides?

Some arguments in favor of a Myriophyllum aquaticum growth inhibition test in a water–sediment system as an additional test in risk assessment of herbicides

Sucrose modifies growth and physiology in axenically grown Myriophyllum spicatum with potential effects on the response to pollutants

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