Effects of tooth storage media on periodontal ligament preservation

Abstract

Adequate thickness of PDL was maintained in the egg white group, whereas the milk group showed disturbance in the PDL, which may lead to ankylosis.

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

“…In another study by Hasan et al . , the regenerated PDL tissue at the root surface of the tooth replanted following immersion in milk, was thinner and showed lower expression of periostin, a protein involved in the process of collagen assembly. In these two studies, whole milk was used, and both showed potential detrimental effects to the process of collagen synthesis.…”

“…So far, HBSS and milk are still the tooth‐preserving solutions recommended as a result of their characteristics and ability to maintain the viability of PDLFs . Nevertheless, each has some disadvantages: HBSS is not easily accessible at the scene of accidents; and milk has been shown to have some negative effects on cells and on the formation of PDL tissue .…”

Effect of propolis on preserving human periodontal ligament cells and regulating pro‐inflammatory cytokines

“…The current study also showed that both BP and HBSS were inferior to milk in maintaining the viability of hPDLCs, which is not consistent with the following studies. Hasan et al compared the effect of HBSS, milk, and egg white, and found that that the number of hPDLCs was reduced, the extracellular matrix degenerated, and the occurrence of ankylosis increased in the milk group. Sousa et al also reported reduced cellularity of hPDLCs after 1 hour of immersion in milk.…”

“…The current study also showed that both BP and HBSS were inferior to milk in maintaining the viability of hPDLCs, which is not consistent with the following studies. Hasan et al 12 Unfavorable storage conditions after extraction (ie, desiccation) also encourage the growth and differentiation of osteogenic cells from the root side of the periodontal ligament, and the selective loss of periodontal ligament fibroblasts. 22 Therefore, it is essential to explore the effect of different storage media in preserving the osteogenic differentiation potential of hPDLCs.…”

“…Storage media should prevent the unpredictable sequelae of inflammatory root resorption or replacement resorption after tooth replantation. Although several studies have analyzed the viability of PDLs in different tooth storage media using in vitro techniques, little research has been carried out on the osteogenic potential and pro‐inflammatory cytokine expression levels in hPDLCs after treatment with different storage media . These two aspects are important for researchers to understand the role of storage media in the reparative processes in hPDLCs after replantation.…”