Effect of propolis on preserving human periodontal ligament cells and regulating pro‐inflammatory cytokines

Yuan, Xiaojing; Wang, Yuanyuan; Shi, Bingqing; Zhao, Yuming

doi:10.1111/edt.12411

Cited by 11 publications

(8 citation statements)

References 35 publications

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“…The vitality of PDLFs placed in both whole and low‐fat cow's milk was significantly higher than that of PDLFs placed in HBSS. These outcomes are consistent with previous reports that used similar experimental methods, showing that milk was more capable than HBSS of maintaining PDLF viability in vitro . However, in some studies, the efficacy of milk is either lower than or comparable with HBSS.…”

Section: Discussionsupporting

confidence: 91%

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Sinpreechanon

Boonzong

Sricholpech

2019

European J Oral Sciences

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Milk remains one of the most frequently recommended solutions for storage of avulsed teeth because it can maintain cell viability and is easily accessible. However, some negative effects of milk on avulsed teeth have been reported, just as the effects of milk on the long‐term functions of cells are not clear. This study aimed to evaluate the effects of different types of milk on the viability, proliferation, and functions of periodontal ligament fibroblasts (PDLF)s in vitro. Human PDLFs were culture‐medium depleted for 5 min and stored in Hanks’ balanced salt solution (HBSS), whole cow's milk, low‐fat cow's milk, or almond milk for 1 h at 25°C. Cell viability and proliferation were assessed using MTT assays. Expression of the genes encoding type I collagen and its modifying enzymes were analyzed using real‐time PCR. Collagen matrix production was evaluated using Picrosirius red polarization. Our results showed the overall efficiency of low‐fat cow's milk in maintaining the viability and proliferation of PDLFs, and in enhancing the process of collagen production. Almond milk storage resulted in the highest rate of PDLF proliferation, and comparable collagen biosynthesis ability to the control. Therefore, besides low‐fat cow's milk, almond milk may potentially be an alternative tooth‐storage medium for PDLF preservation and PDL tissue regeneration.

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Section: Discussionsupporting

confidence: 91%

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Sinpreechanon

Boonzong

Sricholpech

2019

European J Oral Sciences

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“…The results of this study were not consistent with those reported in most of studies conducted to investigate the hepatoprotective effects of Propolis [11,12,13,14,32].…”

Section: Discussioncontrasting

confidence: 99%

“…Therefore, it reduces inflammation-induced liver damages by its anti-inflammatory effects. [12] Furthermore, Propolis reduces CRP serum level which is considered as a proinflammatory indicator [13].…”

Section: Introductionmentioning

confidence: 99%

Histopathological Examination of the Effects of Oral Consumption of Various Doses of Propolis in Mice Liver

Samiee-Rad¹,

Bagherkhani²,

Gheibi³

2019

JFNR

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Introduction: Considering the widespread application of biological substances, such as Propolis, it is essential to identify the importance of its liver metabolism, its effects of the liver and safe doses. The aim of this study was to determine the histopathological effects of oral administration of Propolis on the liver in mice. Methods and materials: This study consisted of five experimental groups of male mice (25-30 g), including control, placebo and 3 treatment groups. Male mice (25-30 g). Each of the groups receiving Propolis extract at one of the 2000, 4000 and 8000 mg/kg concentrations. Mice were gavage fed with the alcoholic extract of Iranian Propolis once daily for 14 days. After induction of a deep anesthesia, the liver was removed and examined for histopathological changes. All the data were analyzed using SPSS software (P <0.05). Results: The results of this study indicated that chronic administration of various doses of routine Propolis induced different histopathological changes in a dose-dependent manner. These changes initiated around the central venous; furthermore, their severity was higher in this part of the liver compare to the other areas. Conclusion: Based on the results of this study, it is essential to carefully monitor the dosage and consumption duration of Propolis. Moreover, periodical examination of liver function is essential during chronic consumption of Propolis.

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“…Some substances tested to treat the root surface before delayed tooth replantation have efficacy as osteoporotic therapeutic agents that inhibit osteoclast activity and modulate immune inflammatory reactions. 6,7,8 These substances include Emdogain, alendronate, odanacatib 6 and propolis. 7,8 In recent years, compounds of natural origin, obtained from plants such as curcumin and piperine, have been tested to formulate anti-inflammatory and antiosteoclastogenic drugs having the potential to treat and prevent replacement resorption of replanted teeth.…”

Section: Introductionmentioning

confidence: 99%

“…6,7,8 These substances include Emdogain, alendronate, odanacatib 6 and propolis. 7,8 In recent years, compounds of natural origin, obtained from plants such as curcumin and piperine, have been tested to formulate anti-inflammatory and antiosteoclastogenic drugs having the potential to treat and prevent replacement resorption of replanted teeth. 9 In this respect, a recent study tested the effect of a plant lectin on stimulated gingival fibroblasts, with promising results 10 for future use as a possible therapy for delayed replanted teeth.…”

Section: Introductionmentioning

confidence: 99%

Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study

et al. 2020

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This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy-Δ12,14 -PG J2 (15d-PGJ 2 ), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGFβ-1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated with LPS 10 μg/ml and treated with 15d-PGJ 2 1 and 2 μg/ml, and ScLL 2 and 5 μg/ml, for 1 and 3h, and then evaluated for viability by MTT assay. Supernatant was collected to detect IL-6 and TGFβ-1 release, by ELISA. Positive control was cells kept in Dulbecco's Modified Eagle's Medium, and negative control was those kept in LPS. Data were analyzed by ANOVA and Dunnett's test (α = 0.05). No significant difference was found in viability among experimental groups at 1h (p > 0.05). Percentage of ScLL 5 µg/ml viable cells was similar to that of positive control at evaluated periods (p > 0.05), whereas the other groups had lower levels than the positive control (p < 0.05). IL-6 release was statistically higher for ScLL 5 μg/ml and 15d-PGJ 2 2 µg/ml at 1h, compared with the other treated groups and positive control (p < 0.05). No significant differences were found among the groups at 3h (p > 0.05), except for ScLL 2 µg/ml and 15d-PGJ 2 1 µg/ml, which showed lower IL-6 release compared with that of negative control (p < 0.05). No significant difference was found among the groups for TGFβ-1 release (p > 0.05). Results indicated that ScLL 5 μg/ml did not interfere in viability, and ScLL 2 µg/ml and 15d-PGJ 2 1 µg/ml demonstrated reduced IL-6 release. Tested substances had no effect on TGFβ-1 release.

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Effect of propolis on preserving human periodontal ligament cells and regulating pro‐inflammatory cytokines

Cited by 11 publications

References 35 publications

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Comparative evaluation of periodontal ligament fibroblasts stored in different types of milk: effects on viability and biosynthesis of collagen

Histopathological Examination of the Effects of Oral Consumption of Various Doses of Propolis in Mice Liver

Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study

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