Effects of Toluidine Blue and Destaining Reagents Used in Sexual Assault Examinations on the Ability to Obtain DNA Profiles from Postcoital Vaginal Swabs

Hochmeister, Manfred N.; Whelan, Margaret; Borer, Urs V.; Gehrig, Christian; Berzlanovich, Andrea; Rauch, E.; Dirnhofer, Richard

doi:10.1520/jfs14119j

Cited by 22 publications

(6 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been reported that dyes coextracted with the DNA can reduce the efficiency of the polymerase chain reaction. 13 With regard to these two rape cases, it has been shown that the DNA obtained from the vaginal smear slides, using the methodology described in this work, was adequate for performing human DNA typing with confidence, thereby allowing reliable and interpretable results to be applied during routine forensic analysis.…”

Section: Discussionmentioning

confidence: 91%

DNA typing from vaginal smear slides in suspected rape cases

et al. 2004

View full text Add to dashboard Cite

In an investigation of suspected rape, proof of sexual assault with penetration is required. In view of this, detailed descriptions of the genitalia, the thighs and pubic region are made within the forensic medical service. In addition, vaginal swabs are taken from the rape victim and some of the biological material collected is then transferred to glass slides. In this report, we describe two rape cases solved using DNA typing from cells recovered from vaginal smear slides. In 1999, two young women informed the Rio de Janeiro Police Department that they had been victims of sexual assaults. A suspect was arrested and the victims identified him as the offender. The suspect maintained that he was innocent. In order to elucidate these crimes, vaginal smear slides were sent to the DNA Diagnostic Laboratory for DNA analysis three months after the crimes, as unique forensic evidence. To get enough epithelial and sperm cells to perform DNA analysis, we used protocols modified from the previously standard protocols used for DNA extraction from biological material fixed on glass slides. The quantity of cells was sufficient to perform human DNA typing using nine short tandem repeat (STR) loci. It was 3.3 billion times more probable that it was the examined suspect who had left sperm cells in the victims, rather than any other individual in the population of Rio de Janeiro.

show abstract

Section: Discussionmentioning

confidence: 91%

DNA typing from vaginal smear slides in suspected rape cases

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Da mesma forma, o teste de Collins utiliza solução de azul de toluidina que, sobre áreas de solução de continuidade da pele ou da mucosa, se fixa após a aplicação do ácido acético. Nas adolescentes, o teste pode duplicar o percentual de achados relacionados com a violência sexual 28 . Essas metodologias, no entanto, não são aplicadas de forma rotineira nos exames médico-legais.…”

Section: Discussionunclassified

Influência do exame médico-legal na responsabilização do autor da violência sexual contra adolescentes

Drezett

Junqueira

Tardelli

et al. 2011

J. Hum. Growth Dev.

View full text Add to dashboard Cite

OBJETIVO: avaliar a influência do exame médico-legal na responsabilização criminal de acusados de violência sexual contra adolescentes do sexo feminino. MÉTODO: foram analisados retrospectivamente 137 processos judiciais de estupro contra adolescentes matriculadas no Centro de Referência da Criança e do Adolescente entre janeiro de 1995 e dezembro de 2004. Os laudos do Instituto Médico Legal foram classificados como negativos ou positivos para a materialidade do crime sexual e confrontados com a sentença judicial, condenatória ou não para o acusado. Os dados foram informatizados em Programa EpiInfo e submetidos ao teste de Qui-quadrado para Tabelas de contingência, fixando-se em p < 0,05 o nível de rejeição da hipótese de nulidade. RESULTADOS: em 30 casos (21,9%) o laudo foi concordante com a queixa de estupro. Em 107 casos (78,1%) o exame médico-legal não encontrou evidência material do crime sexual. Entre os exames positivos, 25 acusados (83,3%) foram condenados, enquanto nas perícias negativas ocorreram 68 condenações (63,5%). CONCLUSÕES: o exame médico-legal positivo se associou com maior probabilidade de condenação do acusado da violência sexual. A ausência de elementos comprobatórios materiais não impediu a responsabilização de parte expressiva dos acusados, indicando que outros meios de convencimento são admitidos pela justiça.

show abstract

“…The result of the study reported no adverse effects on DNA detection and identification with the use of toluidine blue. 14…”

Section: Effects On Dna Detectionmentioning

confidence: 99%

Do we miss half of the injuries sustained during rape because we cannot see them? An overview of the use of toluidine blue tissue stain in the medical assessment of rape cases

Kotze

Brits

2017

South African Family Practice

View full text Add to dashboard Cite

Do we miss half of the injuries sustained during rape because we cannot see them? An overview of the use of toluidine blue tissue stain in the medical assessment of rape cases JM Kotzé & H Brits To cite this article: JM Kotzé & H Brits (2018) Do we miss half of the injuries sustained during rape because we cannot see them? An overview of the use of toluidine blue tissue stain in the medical assessment of rape cases,

show abstract

Effects of Toluidine Blue and Destaining Reagents Used in Sexual Assault Examinations on the Ability to Obtain DNA Profiles from Postcoital Vaginal Swabs

Abstract: Ranch E, Dirnhofer R. Effects of toluidine blue and destaining reagents used in sexual assault examinations on the ability to obtain DNA profiles from postcoital vaginal swabs. J Forensic Sci 1997;42(2)316-319.

Cited by 22 publications

References 17 publications

DNA typing from vaginal smear slides in suspected rape cases

DNA typing from vaginal smear slides in suspected rape cases

Influência do exame médico-legal na responsabilização do autor da violência sexual contra adolescentes

Do we miss half of the injuries sustained during rape because we cannot see them? An overview of the use of toluidine blue tissue stain in the medical assessment of rape cases

Contact Info

Product

Resources

About