Effects of Thyroid Hormone Deficiency on Cyclic Adenosine 3′ : 5′-Monophosphate and Control of Lipolysis in Fat Cells

Armstrong, Kenneth J.; Stouffer, John E.; Inwegen, R. G. Van; Thompson, W. J.; Robison, G. Alan

doi:10.1016/s0021-9258(19)42506-4

Cited by 99 publications

(1 citation statement)

References 41 publications

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“…Οι θυρεοειδικές ορμόνες ενισχύουν την δράση των κατεχολαμινών και διεγείρουν το σύστημα της αδενυλκυκλάσης (Williams et al 1977). Οι δράσεις τους στον μεταβολισμό λιπιδίων και λιπαρών οξέων είναι έμμεσες και ασκούνται μέσω της αδενυλκυκλάσης και της φωσφοδιεστεράσης (Armstrong et al 1974).…”

Section: μηχανισμός δράσης των θυρεοειδικών ορμονών α) βιοχημικές -ενεργιακές επιδράσειςunclassified

Α-Φετοπρωτεινη Και Συγγενης Υποθυρεοειδισμος

Μεγγρελη¹

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Section: μηχανισμός δράσης των θυρεοειδικών ορμονών α) βιοχημικές -ενεργιακές επιδράσειςunclassified

Α-Φετοπρωτεινη Και Συγγενης Υποθυρεοειδισμος

Μεγγρελη¹

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Local and systemic alterations in cyclic 3′,5′ AMP phosphodiesterase activity in relation to tail regeneration under hypothyroidism and T4 replacement in the lizard,Mabuya carinata

1996

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Cyclic nucleotide phosphodiesterase isozymes in neuroblastoma cells

Walz

Holt

Howlett

1987

J of Neuroscience Research

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Adenosine 3',5'-cyclic monophosphate (cAMP) content of neurons is determined not only by the rate of synthesis but also by the rate of hydrolysis by cyclic nucleotide phosphodiesterases. Multiple forms of cyclic nucleotide phosphodiesterase exist in brain and other tissues, and these may be regulated by various hormones and neuromodulators. The present study examines this regulation in a cloned line of neuroblastoma cells (N18TG2). A biphasic Lineweaver-Burk plot of cAMP hydrolysis revealed two Kms approximating 5 and 25 microM. Lineweaver-Burk plots of cGMP hydrolysis were linear over a range of 1 microM to 1 mM and exhibited a Km of 37 microM. Neither cAMP nor cGMP competed for hydrolysis of the alternative cyclic nucleotide. No evidence for an allosteric activation of cAMP phosphodiesterase by cGMP was found. Calcium regulation of phosphodiesterase was not found in spite of preparation of the cell extract with several protease inhibitors, and addition of exogenous calmodulin. No effect of calmodulin antagonists (calmidazolium, W7, or trifluoperazine) was observed in vitro or in situ. Growth of the cells in the presence of 200 nM 3,5,3'-triiodothyronine (T3) resulted in an increased hydrolysis of cAMP but of cGMP. This increase was attributed to an increase in Vmax with no change in either high or low Km. This response was blocked by cycloheximide, suggesting that the thyroid hormone effect requires protein synthesis. The thyroid hormone response in neuroblastoma cells is compared with the results of other studies of thyroid hormone effects on phosphodiesterase in other tissues in vivo.

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Effects of Thyroid Hormone Deficiency on Cyclic Adenosine 3′ : 5′-Monophosphate and Control of Lipolysis in Fat Cells

Cited by 99 publications

References 41 publications

Α-Φετοπρωτεινη Και Συγγενης Υποθυρεοειδισμος

Α-Φετοπρωτεινη Και Συγγενης Υποθυρεοειδισμος

Local and systemic alterations in cyclic 3′,5′ AMP phosphodiesterase activity in relation to tail regeneration under hypothyroidism and T4 replacement in the lizard,Mabuya carinata

Cyclic nucleotide phosphodiesterase isozymes in neuroblastoma cells

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