Effects of thiamine and pyridoxine on respiratory activity in Saccharomyces carlsbergensis strain 4228

Nakamura, Ichiro; Isobe, Naohiko; Kamihara, Teijiro; Fukui, Saburô

doi:10.1007/bf00414354

Cited by 7 publications

(12 citation statements)

References 12 publications

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“…NAD-GDH activity was lower and NADP-GDH activity was markedly higher in the cells which were growing at a decreased rate with added thiamin as compared with those in the control cells. Both vitamin B6 content and respiratory activity were substantially lower in the thiamin-grown cells, as reported previously (Nakamura et al, 1974(Nakamura et al, , 1980(Nakamura et al, , 1981. Addition of pyridoxine to the thiaminsupplemented culture at mid-exponential phase restored the growth rate and respiratory activity to normal levels (Nakamura et al, 1980).…”

Section: Resultssupporting

confidence: 85%

“…Both vitamin B6 content and respiratory activity were substantially lower in the thiamin-grown cells, as reported previously (Nakamura et al, 1974(Nakamura et al, , 1980(Nakamura et al, , 1981. Addition of pyridoxine to the thiaminsupplemented culture at mid-exponential phase restored the growth rate and respiratory activity to normal levels (Nakamura et al, 1980). This also brought about an increase in NAD-GDH activity and, in contrast, a greater and more rapid decrease in NADP-GDH activity (Fig.…”

Section: Resultssupporting

confidence: 85%

“…In contrast to 6-aminolaevulinate, pyridoxine, which also eliminated the thiamin-induced respiratory deficiency (Nakamura et al, 1980), abolished completely the effect of thiamin on NADP-GDH as well as that on NAD-GDH. On the other hand, the thiamin-induced vitamin B6 deficiency was found to have no effect on cellular pools of NH,+ and glutamate which are known to regulate the activities of GDH enzymes.…”

Section: Discussionmentioning

confidence: 82%

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Effect of Thiamin-induced Vitamin B6 Deficiency on NAD- and NADP-linked Glutamate Dehydrogenases in Yeast

Nakamura¹,

Ohmura

Kamihara

1983

Microbiology

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Thiamin-grown cells of Saccharomyces carlsbergensis exhibited decreased activity of NADlinked glutamate dehydrogenase (NAD-GDH) and, in contrast, increased activity of NADPlinked glutamate dehydrogenase (NADP-GDH), as compared with control cells. Normal levels of glutamate dehydrogenase (GDH) activities were restored by adding pyridoxine. 6-Aminolaevulinate also prevented the thiamin-induced decrease in NAD-GDH activity, but had no effect on the increase in NADP-GDH activity. NAD-GDH activity was decreased similarly under anaerobic conditions, but NADP-GDH activity was little affected. High concentrations of glucose brought about a decrease in NAD-GDH activity and an increase in NADP-GDH activity, as observed in the thiamin-grown cells. When glycerol was used as carbon source in place of glucose, the thiamin effect on GDH activities was not marked. These results suggest that GDH enzymes are independently controlled by thiamin. NAD-GDH activity is decreased mainly through the thiamin-induced respiratory deficiency, and the increase in NADP-GDH activity is due to the thiamin-enhanced glucose effect. Amino acid metabolism seemed not to be involved in the thiamin effect on GDH activities since the intracellular pools of NH,+ and glutamate were not altered by thiamin. The activities of glutamateoxaloacetate transaminase and glutamate-pyruvate transaminase were not influenced greatly by thiamin, unlike the activity of b-aminolaevulinate synthase.

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Section: Resultssupporting

confidence: 85%

Section: Resultssupporting

confidence: 85%

Section: Discussionmentioning

confidence: 82%

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Effect of Thiamin-induced Vitamin B6 Deficiency on NAD- and NADP-linked Glutamate Dehydrogenases in Yeast

Nakamura¹,

Ohmura

Kamihara

1983

Microbiology

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“…Thiamin inhibits the growth of Saccharomyces carlsbergensis strain 4228 under aerobic conditions when added to a vitamin B,-free medium, As reported previously (Nagai et al, 1974;Nakamura et al, 1974Nakamura et al, , 1976Nakamura et al, , 1980Nakamura et al, , 1981Nishikawa et al, 1974Nishikawa et al, , 1977Nishikawa et al, , 1978, the thiamininduced growth depression is accompanied by the following sequence of events in the cells : a marked decrease in the vitamin B, content, a pronounced lowering of the activity of 6aminolaevulinate synthase, the absence of 6-aminolaevulinate, porphyrins and cytochromes, marked decreases in the activities of respiratory chain enzymes and other haem-containing enzymes, and an extreme reduction of respiration and a significant alteration in lipid composition. Pyridoxine or other vitamin B6 compounds added concomitantly with thiamin completely abolish these effects of thiamin, and cells grow normally with normal profiles of the above cellular activities (Nagai et al, 1974;Nakamura et al, 1974Nakamura et al, , 1980Nishikawa et al, 1974Nishikawa et al, , 1977.…”

Section: Introductionsupporting

confidence: 75%

“…Pyridoxine or other vitamin B6 compounds added concomitantly with thiamin completely abolish these effects of thiamin, and cells grow normally with normal profiles of the above cellular activities (Nagai et al, 1974;Nakamura et al, 1974Nakamura et al, , 1980Nishikawa et al, 1974Nishikawa et al, , 1977. The addition of 6-aminolaevulinate has the same effects as that of pyridoxine except that growth is not completely restored (Nakamura et al, 1981). The activities of some pyridoxal phosphate-dependent enzymes other than 6-aminolaevulinate synthase may also be reduced by the thiamin-induced vitamin B6 deficiency, and this may lead to disturbance in the metabolism of amino acids, glycogen and polyamines.…”

Section: Introductionmentioning

confidence: 99%

Thiamin Accumulation and Growth Inhibition in Yeasts

Nakamura¹,

Ohmura²,

Nagami³

et al. 1982

Microbiology

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~~Thiamin caused depression of growth, a marked decrease in cellular vitamin B6 content and cytochrome oxidase activity in Saccharomyces yeasts growing in a vitamin B,-free medium under aerobic conditions but had practically no effect in Kluyveromyces, Schizosaccharomyces and Candida spp. Pyridoxine added concomitantly with thiamin permitted the thiaminsensitive yeasts to grow normally with increased activity of cytochrome oxidase. 6-Aminolaevulinate also caused the increase in cytochrome oxidase activity but growth was only partially improved by the addition of this precursor of haem biosynthesis. These phenomena were similar to those found previously in Saccharomyces carlsbergensis strain 4228 (ATCC 9080) (Nakamura et al., 198 1). Thiamin-sensitive yeasts accumulated thiamin more than 24-fold when compared with the thiamin-insensitive cells. Thiamin transported into the thiamin-sensitive yeasts was recovered only in the non-esterified form. Thiamin added to the growth medium was also accumulated by growing cells of the thiamin-sensitive yeasts especially during the lag and early exponential phases of growth. Pyridoxine did not affect either thiamin accumulation or the intracellular form of the transported thiamin.

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Regulation of respiration and its related metabolism by vitamin B1 and vitamin B6 in Saccharomyces yeasts

Kamihara

Nakamura

1984

Immobilized Biocatalysts Saccharomyces Yeasts Wastewater Treament

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Effects of thiamine and pyridoxine on respiratory activity in Saccharomyces carlsbergensis strain 4228

Cited by 7 publications

References 12 publications

Effect of Thiamin-induced Vitamin B6 Deficiency on NAD- and NADP-linked Glutamate Dehydrogenases in Yeast

Effect of Thiamin-induced Vitamin B6 Deficiency on NAD- and NADP-linked Glutamate Dehydrogenases in Yeast

Thiamin Accumulation and Growth Inhibition in Yeasts

Regulation of respiration and its related metabolism by vitamin B1 and vitamin B6 in Saccharomyces yeasts

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