Effects of Therapeutic Doses of Celecoxib on Several Physiological Parameters of Cultured Human Osteoblasts

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs), including cyclooxygenase-2 (COX-2)-selective NSAIDs, are associated with adverse effects on bone tissue. These drugs are frequently the treatment of choice but are the least studied with respect to their repercussion on bone. The objective of this study was to determine the effects of celecoxib on cultured human osteoblasts. Human osteoblasts obtained by primary culture from bone samples were treated with celecoxib at doses of 0.75, 2, or 5μM for 24 h. The MTT technique was used to determine the effect on proliferation; flow cytometry to establish the effect on cell cycle, cell viability, and antigenic profile; and real-time polymerase chain reaction to measure the effect on gene expressions of the differentiation markers RUNX2, alkaline phosphatase (ALP), osteocalcin (OSC), and osterix (OSX). Therapeutic doses of celecoxib had no effect on osteoblast cell growth or antigen expression but had a negative impact on the gene expression of RUNX2 and OSC, although there was no significant change in the expression of ALP and OSX. Celecoxib at therapeutic doses has no apparent adverse effects on cultured human osteoblasts and only inhibits the expression of some differentiation markers. These characteristics may place this drug in a preferential position among NSAIDs used for analgesic and anti-inflammatory therapy during bone tissue repair.

Repercussions of Bisphenol A on the Physiology of Human Osteoblasts

“…Cells were then washed, suspended in 500 µL PBS, and immediately analyzed in a flow cytometer with argon laser (Facs Vantage Becton Dickinson, Palo Alto, Santa Clara, CA, USA) at a wavelength of 488 nm to determine the percentage of fluorescent cells. We calculated the percentage of annexin-positive (apoptotic) cells and PI-positive (necrotic) cells from counts of 2000-3000 cells [40].…”

Testing active membranes for bone regeneration: A review

“…The RNA obtained from the cells is measured by spectrophotometry. Then, the RNA must be transformed into complementary DNA (cDNA) by means of the reverse transcription, in order to avoid working with RNA, a molecule quite instable [49]. The chains of cDNA will then be amplified by PCR following the manufacturer´s instructions of the commercialized kit used.…”

Physicochemical and Biological Characterisation of Diclofenac Oligomeric Poly(3-hydroxyoctanoate) Hybrids as β-TCP Ceramics Modifiers for Bone Tissue Regeneration

“…The analysis of literature data showed that DIC and other drugs from the NSAIDs group inhibits the viability of osteoblast cell lines—both MC3T3-E1 and MG63 as well as in the rabbit model—effectively preventing the processes associated with bone tissue regeneration [ 69 , 70 , 71 ]. In our study, the materials containing half of the therapeutic dose of chemically bonded DIC showed a cell viability over 70%, which, according to ISO 10993-5, confirms an absence of a cytotoxic effect.…”