Effects of the tea catechin epigallocatechin gallate on Porphyromonas gingivalis biofilms

Abstract

Aims: The aim of this study was to investigate the effects of tea catechin epigallocatechin gallate (EGCg) on established biofilms and biofilm formation by Porphyromonas gingivalis, a major pathogen of periodontal disease. Methods and Results: Biofilm cell survival was measured using adenosine triphosphate (ATP) bioluminescence. In the presence of EGCg, the ATP level in cells of established biofilms was significantly decreased compared to the controls (P < 0Á0001). Transmission electron microscopy revealed that EGCg damaged the cell membrane and cell wall of P. gingivalis. Confocal laserscanning microscopy revealed that the proportion of dead cells was higher in biofilms treated with EGCg. Moreover, the effects of subminimal inhibitory concentrations (MICs) of EGCg on P. gingivalis biofilm formation were dosedependent (P < 0Á0001). Conclusion: Our results suggest that EGCg destroys established P. gingivalis biofilms and inhibits biofilm formation. Significance and Impact of the Study: Development of chemical control agents against oral biofilms is necessary, because oral biofilms can be only removed using mechanical debridement. This article indicates that EGCg may represent a novel antibiofilm agent that prevents infections involving bacterial biofilms such as periodontitis.

Efficacy of Mouth Rinse Formulation Based on Cetylpyridinium Chloride 0.1% in the Control of Dental Calculus Buildup

“…Calculus formation is the result of petrification of dental plaque biofilm, with mineral ions provided by bathing saliva or crevicular fluids [10]. Supragingival calculus formation can be controlled by chemical mineralization inhibitors, applied in toothpastes or mouth rinses [11].…”

“…Biofilm cell survival can be measured using the ATP-driven bioluminescence [10]. Using the luciferin substrate and luciferase enzyme, bacterial ATP can be quantified by measuring the release of visible light.…”

“…CariScreen testing swabs were used in conjunction with the CariScreen testing meter for a painless and simple 1-minute chairside bacterial test to assess biofilm cell survival on patients, particularly in measuring the ATP-driven bioluminescence. The CariScreen testing meter was proven to be effective and accurate in detecting the level of the bacterial load in vivo [10].…”

A novel cold atmospheric pressure air plasma jet for peri-implantitis treatment: An <i>in vitro</i> study

“…Cell concentrations were adjusted to 0.5 McFarland turbidity standards (equivalent to 1.5×10 8 colony forming units/ mL). Thereafter, Porphyromonas gingivalis was treated with various CAPAJ parameters and bacterial surface morphology was studied using SEM 41) . Concurrently, changes occurring on titanium specimen roughness after treatment of CAPAJ were observed.…”

Adverse Influences of Antimicrobial Strategy against Mature Oral Biofilm

“…In the field of dentistry, it has also been reported that sub-MICs of antimicrobial agents or compounds can inhibit bacterial attachment [54,56,57], biofilm formation [54,55,57,58], and downregulate virulence genes [54,56,59,60]. Moon et al reported N-acetyl cysteine (NAC) that is an antioxidant possessing anti-inflammatory activities, showed a significant decrease of Prevotella intermedia biofilm formation in the presence of sub-MIC [55].…”