Effects of the surface modification of polyurethane substrates on genotoxicity and blood activation processes

Major, R.; Plutecka, Hanna; Gruszczynska, Anna; Lackner, J.M.; Major, B.

doi:10.1016/j.msec.2017.05.060

Cited by 4 publications

(6 citation statements)

References 13 publications

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“…The same method has been used elsewhere. 26 The test is based on the cone and plate analyser, an instrument validated for clinical use in the evaluation of thrombotic diseases. The shear test was applied at shear rate 1800 s (720 r/min) for 300 s, using a disposable teflon conical rotor.…”

Section: A Simplified Methods To Assess Haemocompatibility Under High mentioning

confidence: 99%

“…The technique of the flow cytometry analyses has been described elsewhere. 26 Briefly, 5 μL aliquots of blood were gently mixed with fluorochrome-conjugated monoclonal antibodies: 5 μL of fluorescein isothiocyanate (FITC)-PAC-1, 5 μL of PE-CD62P and 4 μL of Peridinin-Chlorophyll-protein (PerCP)-CD61 (all from Becton Dickinson, USA) in phosphate buffered saline (PBS) containing 0.2% bovine serum albumin and 2 mM calcium chloride (final volume 35 μL). After 10 min of staining at room temperature, erythrocytes were lysed by the addition of 0.5 mL of lysing solution (FLS; Becton Dickinson), and platelets were centrifuged (1000× g , 6 min) and resuspended in a PBS buffer for further analyses by flow cytometry.…”

Section: Methodsmentioning

confidence: 99%

“…The samples were analysed using an EPICS XL flow cytometer (Beckman Coulter Inc., Brea, CA, USA). The expression of platelet activation markers 26 was measured on CD61 gated objects using the PAC-1 antibody for the conformational change of glycoprotein IIb.IIIa and CD62P for P-selectin. The integrated fluorescence of the activation marker was calculated as a multiplication total of the geometric mean fluorescence by the percentage of marker-positive objects.…”

Section: Methodsmentioning

confidence: 99%

“…The proteolytic activity of generated thrombin against the chromogenic substrate strictly correlates with the concentration of microparticles present in blood plasma. 26…”

Section: Methodsmentioning

confidence: 99%

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Surface modification of metallic materials designed for a new generation of artificial heart valves

et al. 2018

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Section: A Simplified Methods To Assess Haemocompatibility Under High mentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The proteolytic activity of generated thrombin against the chromogenic substrate strictly correlates with the concentration of microparticles present in blood plasma. 26…”

Section: Methodsmentioning

confidence: 99%

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Surface modification of metallic materials designed for a new generation of artificial heart valves

et al. 2018

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“…Coating of titanium-based substrates was performed by physical vapor deposition and plasma-enhanced chemical vapor deposition (PECVD). A more detailed description is presented elsewhere [6]. Briefly, substrates were ultrasonically cleaned in alcohol and acetone to remove any contaminants on the surface.…”

Section: Surface Modificationmentioning

confidence: 99%

Biocompatibility testing of composite biomaterial designed for a new petal valve construction for pulsatile ventricular assist device

Major

Gawlikowski

Plutecka

et al. 2021

J Mater Sci: Mater Med

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This paper presents the results of biocompatibility testing performed on several biomaterial variants for manufacturing a newly designed petal valve intended for use in a pulsatile ventricular assist device or blood pump. Both physical vapor deposition (PVD) and plasma-enhanced chemical vapor deposition (PECVD) were used to coat titanium-based substrates with hydrogenated tetrahedral amorphous carbon (ta-C:H) or amorphous hydrogenated carbon (a-C:H and a-C:H, N). Experiments were carried out using whole human blood under arterial shear stress conditions in a cone-plate analyzer (ap. 1800 1/s). In most cases, tested coatings showed good or very good haemocompatibility. Type a-C:H, N coating proved to be superior in terms of activation, risk of aggregation, and the effects of generating microparticles of apoptotic origin, and also demonstrated excellent mechanical properties. Therefore, a-C:H, N coatings were selected for further in vivo studies. In vivo animal studies were carried out according to the ISO 10993 standard. Intradermal reactivity was assessed in three rabbits and sub-acute toxicity and local effects after implantation were examined in 12 rabbits. Based on postmortem examination, no organ failure or wound tissue damage occurred during the required period of observation. In summary, our investigations demonstrated high biocompatibility of the biomaterials in relation to thrombogenicity, toxicity, and wound healing. Prototypes of the petal valves were manufactured and mounted on the pulsatile ventricular assist device. Hydrodynamic features and impact on red blood cells (hemolysis) as well as coagulation (systemic thrombogenicity) were assessed in whole blood.

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Effect of the mechanical properties of carbon-based coatings on the mechanics of cell–material interactions

Trembecka-Wójciga

Kopernik

Surmiak

et al. 2021

Colloids and Surfaces B: Biointerfaces

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Effects of the surface modification of polyurethane substrates on genotoxicity and blood activation processes

Cited by 4 publications

References 13 publications

Surface modification of metallic materials designed for a new generation of artificial heart valves

Surface modification of metallic materials designed for a new generation of artificial heart valves

Biocompatibility testing of composite biomaterial designed for a new petal valve construction for pulsatile ventricular assist device

Effect of the mechanical properties of carbon-based coatings on the mechanics of cell–material interactions

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