Effects of the removal of cytoplasm on the development of early cloned bovine embryos

Shi, Hua; Zhang, Hui; Su, Junji; Zhang, Tuo; Quan, Fu Sheng; Liu, Jun; Wang, Yong Sheng; Zhang, Yong

doi:10.1016/j.anireprosci.2011.05.002

Cited by 24 publications

(16 citation statements)

References 32 publications

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“…Several studies have demonstrated that the replication of mammalian mtDNA is initiated at the blastocyst stage (Cummins, 1998;May-Panloup et al, 2005;Spikings et al, 2007) and does not depend on the cell cycle (St John et al, 2010). Furthermore, the replication of mtDNA could increase nuclear transcription levels, producing the energy in the form of ATP that is required for early embryonic development (Hua et al, 2011). In addition, gene reprogramming is most likely ATP dependent (Kikyo et al, 2000), which requires an increase in the level of energy production during the embryonic development.…”

Section: Methodsmentioning

confidence: 97%

Segregation of donor cell mitochondrial DNA in gaur–bovine interspecies somatic cell nuclear transfer embryos, fetuses and an offspring

et al. 2012

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Section: Methodsmentioning

confidence: 97%

Segregation of donor cell mitochondrial DNA in gaur–bovine interspecies somatic cell nuclear transfer embryos, fetuses and an offspring

et al. 2012

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“…The slowest processing time was less than 5 s. In mammalian cells, the average diameter of the nucleus is approximately 6 μm, which occupies about 10% of the total cell volume [23]. Using our approach, the removal volume of the oocyte was 20% on average; the 20% removal of the cytoplasm ratio is significant for early cloned bovine embryos [22]. Depending on the nucleus position, the removal volume can be slightly increased, but the highest volume removed was 36%.…”

Section: Separation Time and Removal Proportion Evaluationmentioning

confidence: 98%

“…It is crucial to remove the nucleus in the smallest volume possible to increase the potential for the development of the nuclear transfer embryos [22]. The MMT controls the volume of the suctioned oocyte by closing the channel after a certain amount of time.…”

Section: Cutting Volume Estimationmentioning

confidence: 99%

On-Chip Enucleation of Bovine Oocytes using Microrobot-Assisted Flow-Speed Control

et al. 2013

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Abstract:In this study, we developed a microfluidic chip with a magnetically driven microrobot for oocyte enucleation. A microfluidic system was specially designed for enucleation, and the microrobot actively controls the local flow-speed distribution in the microfluidic chip. The microrobot can adjust fluid resistances in a channel and can open or close the channel to control the flow distribution. Analytical modeling was conducted to control the fluid speed distribution using the microrobot, and the model was experimentally validated. The novelties of the developed microfluidic system are as follows: (1) the cutting speed improved significantly owing to the local fluid flow control; (2) the cutting volume of the oocyte can be adjusted so that the oocyte undergoes less damage; and (3) the nucleus can be removed properly using the combination of a microrobot and hydrodynamic forces. Using this device, we achieved a minimally invasive enucleation process. The average enucleation time was 2.5 s and the average removal volume ratio was 20%. The proposed new system has the advantages of better operation speed, greater cutting precision, and potential for repeatable enucleation.

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“…Moreover, the less the amount of removed cytoplasm is, the less the amount of removed mitochondria is, the ATP can be generated sufficiently, which benefits the development of cloned embryos. Furthermore, when much cytoplasm was used as nuclear recipients, cloned embryos would contained more blastomeres at the stages of morula and blastocyst, and this maybe also benefits the subsequent development of cloned embryos (Hua et al 2011;Panda et al 2011).…”

Section: The Reprogramming Process In Cloned Embryosmentioning

confidence: 99%

“…The performers with high-level skills of SCNT can avoid large quantity of cytoplasm to be removed in the procedure of enucleation, which benefits the reprogramming of somatic nucleus and the development of cloned embryos. For example, in the procedure of enucleation, removal of >10% cytoplasm volume could decrease the developmental ability of bovine-cloned embryos (Hua et al 2011). …”

Section: The Reprogramming Process In Cloned Embryosmentioning

confidence: 99%

Somatic cell reprogrammed by oocyte: process and barricade

Wang

et al. 2014

Animal Cells and Systems

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Somatic cell nuclear transfer (SCNT) is a technology in which a somatic nucleus is transferred into the cytoplasm of a matured oocyte -reconstructed embryos have the capacity to develop to term. Why somatic cells can be reprogrammed by oocytes -the answer for this question must exist in the cytoplasm of matured oocytes. In this review, totipotent characters of matured oocytes were discussed, which may confer matured oocytes with the capacity to reprogram somatic nucleus. Moreover, the procedure of SCNT also makes a possibility for somatic nucleus to be reprogrammed by oocytes. Compared with fertilized embryos, embryos derived from SCNT exhibit low developmental ability; the barricades in reprogramming process and their possible reasons were also discussed. This review maybe can benefit the mechanism research of SCNT technology and can make contribution for improving the efficiency of this technology.

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Effects of the removal of cytoplasm on the development of early cloned bovine embryos

Cited by 24 publications

References 32 publications

Segregation of donor cell mitochondrial DNA in gaur–bovine interspecies somatic cell nuclear transfer embryos, fetuses and an offspring

Segregation of donor cell mitochondrial DNA in gaur–bovine interspecies somatic cell nuclear transfer embryos, fetuses and an offspring

On-Chip Enucleation of Bovine Oocytes using Microrobot-Assisted Flow-Speed Control

Somatic cell reprogrammed by oocyte: process and barricade

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