Effects of the peptide Magainin H2 on Supported Lipid Bilayers studied by different biophysical techniques

Marín-Medina, Nathaly; Mescola, Andrea; Alessandrini, Andrea

doi:10.1016/j.bbamem.2018.10.003

Cited by 16 publications

(18 citation statements)

References 66 publications

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“…First, the lateral expansion of the membrane induced by the interaction with the AMP is inhibited. Further, it proceeds to create new bilayer regions with many defects in the membrane [ 79 , 80 ].…”

Section: Resultsmentioning

confidence: 99%

A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation

Wieland

Assmann

Bethe

et al. 2021

Sensors

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The increasing rate of antimicrobial resistance (AMR) in pathogenic bacteria is a global threat to human and veterinary medicine. Beyond antibiotics, antimicrobial peptides (AMPs) might be an alternative to inhibit the growth of bacteria, including AMR pathogens, on different surfaces. Biofilm formation, which starts out as bacterial adhesion, poses additional challenges for antibiotics targeting bacterial cells. The objective of this study was to establish a real-time method for the monitoring of the inhibition of (a) bacterial adhesion to a defined substrate and (b) biofilm formation by AMPs using an innovative thermal sensor. We provide evidence that the thermal sensor enables continuous monitoring of the effect of two potent AMPs, protamine and OH-CATH-30, on surface colonization of bovine mastitis-associated Escherichia (E.) coli and Staphylococcus (S.) aureus. The bacteria were grown under static conditions on the surface of the sensor membrane, on which temperature oscillations generated by a heater structure were detected by an amorphous germanium thermistor. Bacterial adhesion, which was confirmed by white light interferometry, caused a detectable amplitude change and phase shift. To our knowledge, the thermal measurement system has never been used to assess the effect of AMPs on bacterial adhesion in real time before. The system could be used to screen and evaluate bacterial adhesion inhibition of both known and novel AMPs.

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Section: Resultsmentioning

confidence: 99%

A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation

Wieland

Assmann

Bethe

et al. 2021

Sensors

View full text Add to dashboard Cite

show abstract

“…For peptide-bound membranes a much lower force is needed to puncture through the bilayer. [89,97,101,102] In some cases, destabilisation of the membrane occurs at very low peptide concentrations. [97] In other cases a threshold peptide concentration is needed, below which peptide addition increases the force needed to puncture the membrane, presumably because peptide binding to the membrane surface increases the lateral pressure at the surface.…”

Section: Mechanical Destabilisationmentioning

confidence: 99%

“…[97] In other cases a threshold peptide concentration is needed, below which peptide addition increases the force needed to puncture the membrane, presumably because peptide binding to the membrane surface increases the lateral pressure at the surface. [101,102] To date, there have been few studies comparing the relationship between bilayer stability and topographical defects formed. In one, the extent of destabilisation correlated with the number of nanoscale pits was observed.…”

Section: Mechanical Destabilisationmentioning

confidence: 99%

Atomic force microscopy to elucidate how peptides disrupt membranes

Hammond

Ryadnov

Hoogenboom

2021

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Atomic force microscopy is an increasingly attractive tool to study how peptides disrupt membranes.Often performed on reconstituted lipid bilayers, it provides access to time and length scales that allow dynamic investigations with nanometre resolution. Over the last decade, AFM studies have enabled visualisation of membrane disruption mechanisms by antimicrobial or host defence peptides, including peptides that target malignant cells and biofilms. Moreover, the emergence of high-speed modalities of the technique broadens the scope of investigations to antimicrobial kinetics as well as the imaging of peptide action on live cells in real time. This review describes how methodological advances in AFM facilitate new insights into membrane disruption mechanisms.

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“…Several ways of exploiting the AMPs properties, such as Magainin H2, have a high level of hydrophobicity and present a good permeabilization action on lipid bilayers. Thus, experimental and theoretical studies investigated how AMPs and lipid bilayers interact, concluding that, at first, the lateral growth of the membrane caused by interactions with the AMP is inhibited, but in the end, it generates new bilayer regions that have defects in their membrane [69,70]. Another feasible option is the combined use of AMPs and conventional antibiotics, showing synergy and lowering microbial resistance [71].…”

Section: Antimicrobial Agents and Antimicrobial Resistancementioning

confidence: 99%

Preventing Biofilm Formation and Development on Ear, Nose and Throat Medical Devices

et al. 2021

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Otorhinolaryngology is a vast domain that requires the aid of many resources for optimal performance. The medical devices utilized in this branch share common problems, such as the formation of biofilms. These structured communities of microbes encased in a 3D matrix can develop antimicrobial resistance (AMR), thus making it a problem with challenging solutions. Therefore, it is of concern the introduction in the medical practice involving biomaterials for ear, nose and throat (ENT) devices, such as implants for the trachea (stents), ear (cochlear implants), and voice recovery (voice prosthetics). The surface of these materials must be biocompatible and limit the development of biofilm while still promoting regeneration. In this respect, several surface modification techniques and functionalization procedures can be utilized to facilitate the success of the implants and ensure a long time of use. On this note, this review provides information on the intricate underlying mechanisms of biofilm formation, the large specter of implants and prosthetics that are susceptible to microbial colonization and subsequently related infections. Specifically, the discussion is particularized on biofilm development on ENT devices, ways to reduce it, and recent approaches that have emerged in this field.

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Effects of the peptide Magainin H2 on Supported Lipid Bilayers studied by different biophysical techniques

Cited by 16 publications

References 66 publications

A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation

A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation

Atomic force microscopy to elucidate how peptides disrupt membranes

Preventing Biofilm Formation and Development on Ear, Nose and Throat Medical Devices

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