Effects of the mycotoxin ochratoxin A and some of its metabolites on human kidney cell lines

Müller, Gertrud; Burkert, Beate; Rosner, H.; Köhler, Heike

doi:10.1016/s0887-2333(03)00053-5

Cited by 18 publications

(11 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…OTC is extremely toxic to animals, such as pig, poultry [464] and dairy cattle [467]. In cell culture it has been recently demonstrated that OTC is more toxic than OTA [468,469]. OTC in THP-1 cell (human monocyte/ macrophage line) showed a stronger suppressive effect on most of the functions (inhibition of mitochondrial activity, inhibition of phagocytic capacity of macrophages).…”

Section: Other Mechanismsmentioning

confidence: 99%

Ochratoxin A: An overview on toxicity and carcinogenicity in animals and humans

Pfohl‐Leszkowicz¹,

Manderville²

2006

Molecular Nutrition Food Res

886

525

View full text Add to dashboard Cite

Ochratoxin A (OTA) is a ubiquitous mycotoxin produced by fungi of improperly stored food products. OTA is nephrotoxic and is suspected of being the main etiological agent responsible for human Balkan endemic nephropathy (BEN) and associated urinary tract tumours. Striking similarities between OTA-induced porcine nephropathy in pigs and BEN in humans are observed. International Agency for Research on Cancer (IARC) has classified OTA as a possible human carcinogen (group 2B). Currently, the mode of carcinogenic action by OTA is unknown. OTA is genotoxic following oxidative metabolism. This activity is thought to play a central role in OTA-mediated carcinogenesis and may be divided into direct (covalent DNA adduction) and indirect (oxidative DNA damage) mechanisms of action. Evidence for a direct mode of genotoxicity has been derived from the sensitive 32P-postlabelling assay. OTA facilitates guanine-specific DNA adducts in vitro and in rat and pig kidney orally dosed, one adduct comigrates with a synthetic carbon (C)-bonded C8-dG OTA adduct standard. In this paper, our current understanding of OTA toxicity and carcinogenicity are reviewed. The available evidence suggests that OTA is a genotoxic carcinogen by induction of oxidative DNA lesions coupled with direct DNA adducts via quinone formation. This mechanism of action should be used to establish acceptable intake levels of OTA from human food sources.

show abstract

Section: Other Mechanismsmentioning

confidence: 99%

Ochratoxin A: An overview on toxicity and carcinogenicity in animals and humans

Pfohl‐Leszkowicz¹,

Manderville²

2006

Molecular Nutrition Food Res

886

525

View full text Add to dashboard Cite

show abstract

“…Furthermore, an antibacterial assay with B. brevis resulted in minimum toxic doses (MTDs, nmol disc −1 ) of 8.7 ± 1.7 and 2.0 ± 0.5 for OTA and OTC, respectively [ 86 ]. OTC was also identified as comparably cytotoxic to OTA in human embryonic (HEK293) and renal carcinoma (A498) cell lines [ 184 ]. Based on the above results, OTC is considered to be similarly toxic to OTA.…”

Section: Differential Toxicity Of Ochratoxin Group Membersmentioning

confidence: 99%

Comparative Ochratoxin Toxicity: A Review of the Available Data

Heussner

Bingle

2015

Toxins

268

160

View full text Add to dashboard Cite

Ochratoxins are a group of mycotoxins produced by a variety of moulds. Ochratoxin A (OTA), the most prominent member of this toxin family, was first described by van der Merwe et al. in Nature in 1965. Dietary exposure to OTA represents a serious health issue and has been associated with several human and animal diseases including poultry ochratoxicosis, porcine nephropathy, human endemic nephropathies and urinary tract tumours in humans. More than 30 years ago, OTA was shown to be carcinogenic in rodents and since then extensive research has been performed in order to investigate its mode of action, however, this is still under debate. OTA is regarded as the most toxic family member, however, other ochratoxins or their metabolites and, in particular, ochratoxin mixtures or combinations with other mycotoxins may represent serious threats to human and animal health. This review summarises and evaluates current knowledge about the differential and comparative toxicity of the ochratoxin group.

show abstract

“…Due to the fact that ochratoxigenic fungi can produce OTA and its related compounds, it is more realistic to consider that under natural conditions, wine could be contaminated with a mixture of fungal metabolites. OTC is more toxic than OTA in cell cultures (Müller et al, 2003) and it is rapidly converted into OTA in the body (PfohlLeszkowicz et al, 2007). OTB has demonstrated its cytotoxicity (Dietrich, O'Brien, Stack & Heussner, 2001), nephrotoxicity (Mally et al, 2005) and teratogenicity (O'Brien, Prietz & Dietrich, 2005).…”

Section: Introductionmentioning

confidence: 99%