Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer

Brito, Eric Saymom Andrade; Prado, Laís Di Paulie Taborda; Araújo, Liana Késia Costa; Matos, Manuela; Paula, Joelma Abadia Marciano de; Ramos, Luciana M.; Fonseca‐Alves, Carlos Eduardo; Moura, Veridiana Maria Brianezi Dignani de

doi:10.3389/fvets.2021.605286

Cited by 5 publications

(5 citation statements)

References 39 publications

(51 reference statements)

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“…The difference in pH, based on the results obtained does not show great impact, leaving both JB (Synadenium Umbellatur) and JC (Sinadenium grantii) with similar values and classifying with the same pH according to the reference scale, such as 4.71±1.015 and 4.19±0.34 respectively. In this acidic way, that is, these values do not favor pathogenic bacterial growth, similar, since there was no statistical difference at the level of 5% significance from one variety to the other, these results are in agreement with what is cited by Brito et al (2021), who state in their studies that these pH values provide balance to the product after its removal from the stem, thus delaying microbial development and multiplication.…”

Section: Physicochemical Characterizationsupporting

confidence: 91%

“…The same result was found for the determination of acidity, where Table 2 shows that all analyses showed the same result, as shown in the example. Acidity is a parameter used to guide the quality of the sample, that is, an excellent and very important tool to determine signs of the presence of organic acids, as mentioned by Brito et al (2021), which is not indicated by the results in Table 2.…”

Section: Physicochemical Characterizationmentioning

confidence: 99%

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Physical, physicochemical and phytochemical characterization of the latex of the Janaúba plant (Euphorbia Umbellata) of the varieties Synadenium granti hook and Synadenium umbellatur pax

Pires,

Conceição,

Silva

et al. 2023

Interdisciplinarity and Innovation in Scientific Research

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The use of medicine plants for the healing is used since the begginings, but the rise of the reseraches and the advance of the science, most of the these plants already have the evidenced effectiveness by scientific evidences checked. Therefore, a study about the plant Euphorbia umbellata and its varieties would have great relevance, since it is popularly very known when it comes to its physiotherapeutic action in the ancologic treatment. The species belonging to this group posses great highlight not inly in the economic acticity directly related to the human alimentation but also in the popular medicine based on the empiric knowledge. To characterize physical-chemical and phatochemical the latex of the Janauba plant (Euphorbia umbellata) of the varieties Synadenium grant Hook and Synademium umbellatur Pax. The sampling stock used in the research was collected in Belém and municipalities and Castanhal city, in Pará state, Brazil, between April and November pf 2022. It was taken to the controll and quality laboratory of Centro Universitário da Amazônia (UniEsamaz) located in Belém, PA, for the execution of the analyzes. The anatomic characterization is fairly important because it must be done before the use related to the medicine plant, in what way it can be idetified in other not to occur a wrong consume of the specie. The sample results demonstrate that the latex of Janauba is a product classified as medium acidity, the difference of PH, based on the results obtained do not demonstrate great impact. Thus, it is concluded that despite they have not detected in the current analyses and comparing to the previous results, the Janauba plant and its varieties can be considered as a strong alternative to help the cancer comba and being extremaly essential when it is used due to the presence of cumarinas which can cause hepatic problems for the use of big quantities.

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Section: Physicochemical Characterizationsupporting

confidence: 91%

Section: Physicochemical Characterizationmentioning

confidence: 99%

Physical, physicochemical and phytochemical characterization of the latex of the Janaúba plant (Euphorbia Umbellata) of the varieties Synadenium granti hook and Synadenium umbellatur pax

Pires,

Conceição,

Silva

et al. 2023

Interdisciplinarity and Innovation in Scientific Research

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show abstract

“…Latex from Euphorbia umbellata exhibited cytotoxicity activity against HeLa and HRT-18 cells, melanoma cells (B16F10), and hepatocellular carcinoma. Additionally, in an in vivo study, minimization tumor mass in mice was observed with latex application [ 13 ]. Recently, numerous natural flavonoids such as epicatechin, luteolin, rutin, quercetin, naringenin, hesperidin, baicalin, and diosmin were applied for the management of SARS-CoV-2 [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Anticancer, Anticoagulant, Antioxidant and Antimicrobial Activities of Thevetia peruviana Latex with Molecular Docking of Antimicrobial and Anticancer Activities

et al. 2022

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Natural origin molecules represent reliable and excellent sources to overcome some medicinal problems. The study of anticancer, anticoagulant, and antimicrobial activities of Thevetia peruviana latex were the aim of the current research. An investigation using high-performance liquid chromatography (HPLC) revealed that the major content of the flavonoids are rutin (11.45 µg/mL), quersestin (7.15 µg/mL), naringin (5.25 µg/mL), and hisperdin (6.07 µg/mL), while phenolic had chlorogenic (12.39 µg/mL), syringenic (7.45 µg/mL), and ferulic (5.07 µg/mL) acids in latex of T. peruviana. Via 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging, the experiment demonstrated that latex had a potent antioxidant activity with the IC50 43.9 µg/mL for scavenging DPPH. Hemolysis inhibition was 58.5% at 1000 µg/mL of latex compared with 91.0% at 200 µg/mL of indomethacin as positive control. Negligible anticoagulant properties of latex were reported where the recorded time was 11.9 s of prothrombin time (PT) and 29.2 s of the activated partial thromboplastin time (APTT) at 25 µg/mL, compared with the same concentration of heparin (PT 94.6 s and APPT 117.7 s). The anticancer potential of latex was recorded against PC-3 (97.11% toxicity) and MCF-7 (96.23% toxicity) at 1000 μg/mL with IC50 48.26 μg/mL and 40.31 µg/mL, respectively. Disc diffusion assessment for antimicrobial activity recorded that the most sensitive tested microorganisms to latex were Bacillus subtilis followed by Escherichia coli, with an inhibition zone (IZ) of 31 mm with minimum inhibitory concentration (MIC) (10.2 μg/mL) and 30 mm (MIC, 12.51 μg/mL), respectively. Moreover, Candida albicans was sensitive (IZ, 28 mm) to latex, unlike black fungus (Mucor circinelloides). TEM examination exhibited ultrastructure changes in cell walls and cell membranes of Staphylococcus aureus and Pseudomonas aeruginosa treated with latex. Energy scores of the molecular docking of chlorogenic acid with E. coli DNA (7C7N), and Rutin with human prostate-specific antigen (3QUM) and breast cancer-associated protein (1JNX), result in excellent harmony with the experimental results. The outcome of research recommended that the latex is rich in constituents and considered a promising source that contributes to fighting cancer and pathogenic microorganisms.

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“…Two prostate cell (PC) cultures were established from intact dogs with non-metastatic PC (PC1) and with metastatic PC (PC2). All of the tested latex concentrations had an effect on both PC1 and PC2 cells in a dose-dependent manner ( Brito et al., 2021 ). It is also crucial to note the activity of proteins present in the Euphorbiaceae latex.…”

Section: Anticancer Properties Of Latex-bearing Plant Extractsmentioning

confidence: 99%

Tap the sap – investigation of latex-bearing plants in the search of potential anticancer biopharmaceuticals

Mazur

Bałdysz²,

Warowicka³

et al. 2022

Front. Plant Sci.

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Latex-bearing plants have been in the research spotlight for the past couple of decades. Since ancient times their extracts have been used in folk medicine to treat various illnesses. Currently they serve as promising candidates for cancer treatment. Up to date there have been several in vitro and in vivo studies related to the topic of cytotoxicity and anticancer activity of extracts from latex-bearing plants towards various cell types. The number of clinical studies still remains scarce, however, over the years the number is systematically increasing. To the best of our knowledge, the scientific community is still lacking in a recent review summarizing the research on the topic of cytotoxicity and anticancer activity of latex-bearing plant extracts. Therefore, the aim of this paper is to review the current knowledge on in vitro and in vivo studies, which focus on the cytotoxicity and anticancer activities of latex-bearing plants. The vast majority of the studies are in vitro, however, the interest in this topic has resulted in the substantial growth of the number of in vivo studies, leading to a promising number of plant species whose latex can potentially be tested in clinical trials. The paper is divided into sections, each of them focuses on specific latex-bearing plant family representatives and their potential anticancer activity, which in some instances is comparable to that induced by commonly used therapeutics currently available on the market. The cytotoxic effect of the plant’s crude latex, its fractions or isolated compounds, is analyzed, along with a study of cell apoptosis, chromatin condensation, DNA damage, changes in gene regulation and morphology changes, which can be observed in cell post plant extract addition. The in vivo studies go beyond the molecular level by showing significant reduction of the tumor growth and volume in animal models. Additionally, we present data regarding plant-mediated biosynthesis of nanoparticles, which is regarded as a new branch in plant latex research. It is solely based on the green-synthesis approach, which presents an interesting alternative to chemical-based nanoparticle synthesis. We have analyzed the cytotoxic effect of these particles on cells. Data regarding the cytotoxicity of such particles raises their potential to be involved in the design of novel cancer therapies, which further underlines the significance of latex-bearing plants in biotechnology. Throughout the course of this review, we concluded that plant latex is a rich source of many compounds, which can be further investigated and applied in the design of anticancer pharmaceuticals. The molecules, to which this cytotoxic effect can be attributed, include alkaloids, flavonoids, tannins, terpenoids, proteases, nucleases and many novel compounds, which still remain to be characterized. They have been studied extensively in both in vitro and in vivo studies, which provide an excellent starting point for their rapid transfer to clinical studies in the near future. The comprehensive study of molecules from latex-bearing plants can result in finding a promising alternative to several pharmaceuticals on the market and help unravel the molecular mode of action of latex-based preparations.

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Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer

Cited by 5 publications

References 39 publications

Physical, physicochemical and phytochemical characterization of the latex of the Janaúba plant (Euphorbia Umbellata) of the varieties Synadenium granti hook and Synadenium umbellatur pax

Physical, physicochemical and phytochemical characterization of the latex of the Janaúba plant (Euphorbia Umbellata) of the varieties Synadenium granti hook and Synadenium umbellatur pax

Anticancer, Anticoagulant, Antioxidant and Antimicrobial Activities of Thevetia peruviana Latex with Molecular Docking of Antimicrobial and Anticancer Activities

Tap the sap – investigation of latex-bearing plants in the search of potential anticancer biopharmaceuticals

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