Effects of the Lack of Phosphatidylglycerol on the Donor Side of Photosystem II

Sakurai, Isamu; Mizusawa, Naoki; Ohashi, Shunsuke; Kobayashi, Masami; Wada, Hajime

doi:10.1104/pp.107.098731

Cited by 79 publications

(57 citation statements)

References 53 publications

(65 reference statements)

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“…It was also found that photosynthetic oxygen-evolving activity of dgdA mutant cells was decreased by heat treatment (Fig. 6), which is similar to our previous finding that inactivation of oxygen evolution was induced in PG-depleted pgsA mutant cells (Sakurai et al, 2007). In the PG-depleted pgsA mutant cells, inactivation of oxygen evolution was also observed during dark incubation as in the DpsbO and DpsbV mutant cells (Sakurai et al, 2007).…”

Section: Discussionsupporting

confidence: 89%

“…The cdsA mutant had phenotypes similar to the pgsA mutant. Through detailed analyses of the pgsA mutant, we demonstrated an important role of PG in the acceptor side of PSII at the Q B site (Gombos et al, 2002;Sakurai et al, 2006) as well as in the donor side of PSII for the binding of extrinsic proteins required for sustaining the manganese (Mn) cluster (Sakurai et al, 2007).…”

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confidence: 99%

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Digalactosyldiacylglycerol Is Required for Stabilization of the Oxygen-Evolving Complex in Photosystem II

Sakurai¹,

Mizusawa²,

Wada³

et al. 2007

Plant Physiol.

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128

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The galactolipid digalactosyldiacylglycerol (DGDG) is present in the thylakoid membranes of oxygenic photosynthetic organisms such as higher plants and cyanobacteria. Recent x-ray crystallographic analysis of protein-cofactor supercomplexes in thylakoid membranes revealed that DGDG molecules are present in the photosystem II (PSII) complex (four molecules per monomer), suggesting that DGDG molecules play important roles in folding and assembly of subunits in the PSII complex. However, the specific role of DGDG in PSII has not been fully clarified. In this study, we identified the dgdA gene (slr1508, a ycf82 homolog) of Synechocystis sp. PCC6803 that presumably encodes a DGDG synthase involved in the biosynthesis of DGDG by comparison of genomic sequence data. Disruption of the dgdA gene resulted in a mutant defective in DGDG synthesis. Despite the lack of DGDG, the mutant cells grew as rapidly as the wild-type cells, indicating that DGDG is not essential for growth in Synechocystis. However, we found that oxygen-evolving activity of PSII was significantly decreased in the mutant. Analyses of the PSII complex purified from the mutant cells indicated that the extrinsic proteins PsbU, PsbV, and PsbO, which stabilize the oxygen-evolving complex, were substantially dissociated from the PSII complex. In addition, we found that heat susceptibility but not dark-induced inactivation of oxygen-evolving activity was notably increased in the mutant cells in comparison to the wild-type cells, suggesting that the PsbU subunit is dissociated from the PSII complex even in vivo. These results demonstrate that DGDG plays important roles in PSII through the binding of extrinsic proteins required for stabilization of the oxygen-evolving complex.

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Section: Discussionsupporting

confidence: 89%

mentioning

confidence: 99%

Digalactosyldiacylglycerol Is Required for Stabilization of the Oxygen-Evolving Complex in Photosystem II

Sakurai¹,

Mizusawa²,

Wada³

et al. 2007

Plant Physiol.

Self Cite

128

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“…The results indicate that CS-73 is slightly more sensitive to heat stress-induced inactivation of PSII in the dark than OTcH-1. In cyanobacteria Synechocystis PCC6803 (27)(28)(29) and green alga Chlamydomonas reinhardtii (30), different lipid composition of the thylakoid membranes has been demonstrated to correlate with the thermal stability of PSII at the oxygen production site.…”

Section: Resultsmentioning

confidence: 99%

Different thermal sensitivity of the repair of photodamaged photosynthetic machinery in cultured Symbiodinium species

Takahashi

Whitney

Badger

2009

Proc. Natl. Acad. Sci. U.S.A.

132

127

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Coral bleaching caused by heat stress is accompanied by photoinhibition, which occurs under conditions where the rate of photodamage to photosystem II (PSII) exceeds the rate of its repair, in the symbiotic algae (Symbiodinium spp.) within corals. However, the mechanism of heat stress-induced photoinhibition in Symbiodinium still remains poorly understood. In the present work, we have investigated the effect of elevated temperature on the processes associated with the repair of photodamaged PSII in cultured Symbiodinium (OTcH-1 and CS-73). Severe photoinhibition was observed at temperature exceeding 32°C in Symbiodinium CS-73 cells grown at 25-34°C but not in cultures of the more thermally tolerant Symbiodinium OTcH-1. After photoinhibition treatment by strong light, photodamaged PSII was repaired close to initial levels under low light at 25°C in both OTcH-1 and CS-73. However, the repair was strongly inhibited by increased temperature exceeding 31°C in CS-73 but only weakly in OTcH-1. We found that inhibition of the repair process in CS-73 is attributed to impairment of both protein synthesis-dependent and -independent repair processes and is at least partially caused by suppression of the de novo synthesis of thylakoid membrane proteins and impairment of the generation of ⌬pH across the thylakoid membrane, respectively. Our results suggest that acceleration of photoinhibition by moderate heat stress is attributed primarily to inhibition of the repair of photodamaged PSII and that the photoinhibition sensitivity of Symbiodinium to heat stress is determined by the thermal sensitivity of the PSII repair processes.coral bleaching ͉ heat stress ͉ photoinhibition ͉ photosystem II ͉ zooxanthellae

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“…PG plays an important role in the oligomerization of the light harvesting complex and the dimerization of PSII (McCourt et al, 1985;Krupa et al, 1987;Kruse et al, 2000). It is also required for both the electron-acceptor side and the donor side of PSII (Sakurai et al, 2007). The DBI was increased by drought stress, but decreased by UV-B radiation in leaf PE.…”

Section: Discussionmentioning

confidence: 99%

Impact of UV-B on drought- or cadmium-induced changes in the fatty acid composition of membrane lipid fractions in wheat

Gondor

Szalai

Kovács

et al. 2014

Ecotoxicology and Environmental Safety

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Several reports have demonstrated the direct and indirect effects of UV-B radiation, an integral component of sunlight, on the plant growth and metabolism of various plant species, including a range of morphological, physiological and biochemical changes (Zlatev et al., 2012). However, as well as being a damaging agent UV-B also has an important role as a regulatory signal. The perception of and responses to UV-B by plants stimulate protective mechanisms, including the accumulation of UV-absorbing phenolic compounds and the modification of the biochemical composition (Jenkins, 2008). Under natural conditions plants are often subjected to multiple stress factors, so the impact of any particular stress may be aggravated or attenuated by the simultaneous action of another stressor. In a previous study it was demonstrated that UV-B radiation may have either a positive or negative impact under the same conditions in wheat, depending on the type of secondary abiotic stress factor: Cd or drought (Kovács et al., 2014). Supplemental UV-B light prevented the wilting and leaf rolling induced by PEG treatment. In contrast, combined UV-B and Cd treatment resulted in pronounced oxidative stress, which was manifested in the further enhancement of the leaf MDA content, root antioxidant enzyme activities and also root polyamine content compared to the effect of single stress factors, Cd or UV-B radiation (Kovács et al., 2014).The ability to adjust membrane lipid fluidity by changing the level of unsaturated fatty acids is a feature of stress-tolerant plants, resulting in an environment suitable for the function of critical integral proteins. Changes have been described in the total lipid content, in the saturation of the total lipids, in the ratio of the membrane lipid fractions, and in the fatty acid composition of individual membrane fractions during single abiotic stresses, such as drought (Zhong et al., 2011;Filek et al., 2012) or heavy metal (Pál et al., 2007;Bernat et al., 2014). Nevertheless, no reports have yet been published on the combined effect of either drought or Cd treatment and UV-B radiation on the fatty acid composition of the individual membrane fractions in wheat plants. Impact of UV-B on drought-or cadmium-induced changes in the fatty acid composition of membrane lipid fractions in wheat AbstractUV-B radiation may have either a positive or negative impact under the same conditions in wheat, depending on the type of secondary abiotic stressor: Cd or drought. Supplemental UV-B prevented the wilting and leaf rolling induced by PEG treatment. In contrast, combined UV-B and Cd treatment resulted in pronounced oxidative stress. The opposite effect of UV-B radiation in the case of drought or cadmium stress may be related to the alteration induced in the fatty acid composition. UV-B caused changes in the unsaturation of leaf phosphatidylglycerol fractions, and the accumulation of flavonoid in the leaves may prevent the stress induced by subsequent drought treatment. However it resulted in pronounced injury despite ...

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Effects of the Lack of Phosphatidylglycerol on the Donor Side of Photosystem II

Cited by 79 publications

References 53 publications

Digalactosyldiacylglycerol Is Required for Stabilization of the Oxygen-Evolving Complex in Photosystem II

Digalactosyldiacylglycerol Is Required for Stabilization of the Oxygen-Evolving Complex in Photosystem II

Different thermal sensitivity of the repair of photodamaged photosynthetic machinery in cultured Symbiodinium species

Impact of UV-B on drought- or cadmium-induced changes in the fatty acid composition of membrane lipid fractions in wheat

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