Effects of the inoculant strain Pseudomonas putida KT2442 (pNF142) and of naphthalene contamination on the soil bacterial community

Gomes, Newton C. M.; Кошелева, И. А.; Abraham, Wolf Rainer; Smalla, Kornelia

doi:10.1016/j.femsec.2005.02.005

Cited by 111 publications

(81 citation statements)

References 32 publications

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“…Langworthy et al (1998) found that high PAH concentrations increased the frequencies of nahA and alkB genes. Gomes et al (2005) also reported a positive correlation between naphthalene contamination and abundance of phnAc genes. The increase of these functional genes might be due to the natural selection of organisms capable of utilizing hydrocarbon compounds.…”

Section: Discussionmentioning

confidence: 88%

Functional gene diversity of soil microbial communities from five oil-contaminated fields in China

et al. 2010

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To compare microbial functional diversity in different oil-contaminated fields and to know the effects of oil contaminant and environmental factors, soil samples were taken from typical oil-contaminated fields located in five geographic regions of China. GeoChip, a high-throughput functional gene array, was used to evaluate the microbial functional genes involved in contaminant degradation and in other major biogeochemical/metabolic processes. Our results indicated that the overall microbial community structures were distinct in each oil-contaminated field, and samples were clustered by geographic locations. The organic contaminant degradation genes were most abundant in all samples and presented a similar pattern under oil contaminant stress among the five fields. In addition, alkane and aromatic hydrocarbon degradation genes such as monooxygenase and dioxygenase were detected in high abundance in the oil-contaminated fields. Canonical correspondence analysis indicated that the microbial functional patterns were highly correlated to the local environmental variables, such as oil contaminant concentration, nitrogen and phosphorus contents, salt and pH. Finally, a total of 59% of microbial community variation from GeoChip data can be explained by oil contamination, geographic location and soil geochemical parameters. This study provided insights into the in situ microbial functional structures in oil-contaminated fields and discerned the linkages between microbial communities and environmental variables, which is important to the application of bioremediation in oil-contaminated sites.

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Section: Discussionmentioning

confidence: 88%

Functional gene diversity of soil microbial communities from five oil-contaminated fields in China

et al. 2010

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“…DGGE of the 16S rRNA gene amplicons was performed according to Gomes et al [31]. The gel was silver-stained according to Heuer et al [32].…”

Section: Pcr Amplification Of 16s Rrna Gene Fragments and Dgge Analysismentioning

confidence: 99%

- Discussion on Sustainable Water Technologies for Peri-Urban Areas of Mexico City: Balancing Urbanization and Environmental Conservation

2014

Agricultural Resource Use and Management

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Natural scrublands in semi-arid deserts are increasingly being converted into fields. This results in losses of characteristic flora and fauna, and may also affect microbial diversity. In the present study, the long-term effect (50 years) of such a transition on soil bacterial communities was explored at two sites typical of semi-arid deserts. Comparisons were made between soil samples from alfalfa fields and the adjacent scrublands by two complementary methods based on 16S rRNA gene fragments amplified from total community DNA. Denaturing gradient gel electrophoresis (DGGE) analyses revealed significant effects of the transition on community composition of Bacteria, Actinobacteria, Alpha-and Betaproteobacteria at both sites. PhyloChip hybridization analysis uncovered that the transition negatively affected taxa such as Acidobacteria, Chloroflexi, Acidimicrobiales, Rubrobacterales, Deltaproteobacteria and Clostridia, while Alpha-, Beta-and Gammaproteobacteria, Bacteroidetes and Actinobacteria increased in abundance. Redundancy analysis suggested that the community composition of phyla responding to agricultural use (except for Spirochaetes) correlated with soil parameters that were significantly different between the agricultural and scrubland soil. The arable soils were lower in organic matter and phosphate concentration, and higher in salinity. The variation in the bacterial community composition was higher in soils from scrubland than from agriculture, as revealed by DGGE and PhyloChip analyses, suggesting reduced beta diversity due to agricultural practices. The long-term use for agriculture resulted in profound changes in the bacterial community and physicochemical characteristics of former scrublands, which may irreversibly affect the natural soil ecosystem.

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“…Pseudomonas is a genus of high biotechnological interest and the capacity of this group for the degradation of xenobiotics is widely documented in the literature [5,6,24,32,35,37,43]. A previous study conducted in Ria de Aveiro showed that Pseudomonads represent 34.5% of the culturable fraction of naphthalene-degrading bacterioneuston of the SML of the Ria de Aveiro [14].…”

Section: Pseudomonads Among Surfactant-resistant Bacterioneuston Isolmentioning

confidence: 99%

Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

Louvado

Coelho²,

Domingues³

et al. 2012

J. Microbiol. Biotechnol.

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Effects of the inoculant strain Pseudomonas putida KT2442 (pNF142) and of naphthalene contamination on the soil bacterial community

Cited by 111 publications

References 32 publications

Functional gene diversity of soil microbial communities from five oil-contaminated fields in China

Functional gene diversity of soil microbial communities from five oil-contaminated fields in China

- Discussion on Sustainable Water Technologies for Peri-Urban Areas of Mexico City: Balancing Urbanization and Environmental Conservation

Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

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