Effects of the cross-linker on the performance and stability of enzymatic electrocatalytic films of glucose oxidase and dimethylferrocene-modified linear poly(ethyleneimine)

From Enzyme Stability to Enzymatic Bioelectrode Stabilization Processes

“…The redox potential of the hydrogel matrix can be adapted to the desired reaction to be catalyzed by tuning the ligands of the redox moieties. Ferrocene entity was tethered to linear PEI and used to cross-link GOx [264]. Of initial current, 70% and 36% was retained after 21 days of storage and 6 h of continuous operation, respectively.…”

Mini-Review: Recent Technologies of Electrode and System in the Enzymatic Biofuel Cell (EBFC)

“…Figure 2 also shows that various interaction experiments produce different electrochemical reaction activities. In addition to covalent bonds formed through the amino group, crosslinking agents are also possible through carboxyl (-COOH) groups [42]. Ji et al [43] produced polyethyleneimine (PEI), in which the redox polymer is used to entrap and bind GOx enzyme with F-N/CNT and PEI, thus making physical interaction through electrostatic attraction.…”

“…Ji et al [43] produced polyethyleneimine ( which the redox polymer is used to entrap and bind GOx enzyme with F-N/CNT a thus making physical interaction through electrostatic attraction. PEI offers advant increasing electron transfer because it has a lower glass transition that mobilizes b the polymer structures and has high stability, sensitivity [42], and conductivity addition, US et al [18] minimized enzyme leaching by producing laser-induced gr with CNTs in microfluidic EBFCs. The high porosity and low cost of this material in the active site for the enzyme and give 1.37 times higher power density than w CNTs.…”

“…Pollutants such as atrazine are very harmful to humans and the environment, even at low ppb levels. Therefore, application of the EBFC as a biosensor electrode should have the ability to detect and quantify different analytes, good anti-interference performance [3], and lead to more efficient energy generation [42]. The diagram in Figure 6 shows the working schematic of the self-powered sensors using EBFC.…”

“…mL Ar-saturated 0.1 M SDA (pH 5.0) with 0.05 M glucose; the cathodic compartment was filled with 50 mL 0.1M B-R buffer (pH 5.0) with 0.5 mM ABTS and continuously bubbled with oxygen; discharge time reached 49.9 h at a discharge current of 0.2 mA before the voltage was lower than 0.8 V Open-circuit voltage reached 1.16 V; power density was measured to 0.350 mW/cm 2 , which correlated to the gravimetric power density of 0.265 mW/mg (per mg of GOx) at 0.85 V × 2 mm × 1 mm (length × width × height) Stored at 4 • C; CB bioanode exhibited almost 65.5% of current response of its first day whereas AM bioanode showed about 50% of performance of its first day Enzyme solutions of glucose oxidase (5 mg/mL, pH 7) and laccase (5 mg/mL, pH 5); bioelectrodes were dried in atmospheric conditions for 2 h and preserved in PBS at 4 • C until use; the electrolyte solutions consisted of mediators such as PBQ (1 mM, pH 7) and ABTS (1 mM, pH 5) in anolyte and catholyte, respectively. CB bioelectrodes gave a power density of 0.1 µW/cm 2 with a current density of 3 µA/cm 2 at an open circuit potential of 105 M PBS (pH 7.4) was used as electrolyte and potential scan rate was 20 mV/s at N 2 state condition Membraneless EBFC adopting this catalyst is measured, maximum power density is 24C for 24 h; 48% of the initial OCP value is retained after 21 days of storage; 60% of the initial current density was lost in that period of time Solutions of b-D-glucose in 0.1 M pH 1 4 7.4 PB were prepared at concentrations ranging from 0 to 10 mM in 2 mM increments; applying potentials from 0.3 to þ0.3 V vs. a Ag/AgCl pseudoreference at scan rates (n) of 5e500 mV/s OCP of around 0.82 V, and a maximum current and power of about 440 mA/cm 2 and 86 mW/cm 2[42] …”