Effects of the commercial formulation containing fipronil on the non-target organism Cyprinus carpio: Implications for rice−fish cultivation

Clasen, Bárbara; Loro, Vania Lúcia; Cattaneo, R; Moraes, Bibiana Silveira; Lópes, Thais; Avila, Luis Antônio de; Zanella, Renato; Reimche, Geovane Boschmann; Baldisserotto, Bernardo

doi:10.1016/j.ecoenv.2011.10.001

Cited by 76 publications

(33 citation statements)

References 40 publications

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“…3b, c). In agreement with our findings the activity of SOD increased in the liver carp exposed to carbofuran under rice field and laboratory conditions (Clasen et al 2012). Regarding different GPx isoforms, we could observe an increase in the expression of GPx4 in both S1 and S2 sites (Fig.…”

Section: Stress Markers In Lambari Fishsupporting

confidence: 92%

Oxidative stress markers in fish (Astyanax sp. and Danio rerio) exposed to urban and agricultural effluents in the Brazilian Pampa biome

Costa-Silva

Nunes

Wallau

et al. 2015

Environ Sci Pollut Res

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Aquatic ecosystems are under constant risk due to industrial, agricultural, and urban activities, compromising water quality and preservation of aquatic biota. The assessment of toxicological impacts caused by pollutants to aquatic environment using biomarker measurements in fish can provide reliable data to estimate sublethal effects posed by chemicals in contaminated areas. In this study, fish (Astyanax sp. and Danio rerio) exposed to agricultural and urban effluents at the Vacacaí River, Brazil, were tested for potential signs of aquatic contamination. This river comprehends one of the main watercourses of the Brazilian Pampa, a biome with a large biodiversity that has been neglected in terms of environmental and social-economic development. Sites S1 and S2 were chosen by their proximity to crops and wastewater discharge points, while reference site was located upstream of S1 and S2, in an apparently non-degraded area. Fish muscle and brain tissues were processed for determination of acetylcholinesterase as well as oxidative stress-related biomarkers. The results showed signs of environmental contamination, hallmarked by significant changes in cholinesterase activity, expression of metallothionein, antioxidant enzymes, glutathione levels, and activation of antioxidant/cell stress response signaling pathways in fish exposed to contaminated sites when compared to reference. Based on these results, it is evidenced that urban and agricultural activities are posing risk to the environmental quality of water resources at the studied area. It is also demonstrated that cell stress biomarkers may serve as important tools for biomonitoring and development of risk assessment protocols in the Pampa biome.

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Section: Stress Markers In Lambari Fishsupporting

confidence: 92%

Oxidative stress markers in fish (Astyanax sp. and Danio rerio) exposed to urban and agricultural effluents in the Brazilian Pampa biome

Costa-Silva

Nunes

Wallau

et al. 2015

Environ Sci Pollut Res

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“…In another investigation that examined gonad size following FIP exposure, the GSI in female rats also remained unchanged, although these exposures were associated with other reproductive impacts . In the present study, FIP exposure had no influence on fathead minnow mass in either sex, similar to the results in common carp exposed to FIP . In several other fish studies, FIP exposure had mixed effects on fish mass, decreasing both adult and larval body size ratios in medaka and variously decreasing or increasing larval body mass in 2 different studies with fathead minnows.…”

Section: Discussionsupporting

confidence: 78%

Effects of the insecticide fipronil on reproductive endocrinology in the fathead minnow

Bencic

Villeneuve

Blake

et al. 2013

Enviro Toxic and Chemistry

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Gamma-aminobutyric acid (GABA) and GABA receptors play an important role in neuroendocrine regulation in fish. Disruption of the GABAergic system by environmental contaminants could interfere with normal regulation of the hypothalamic-pituitary-gonadal axis, leading to impaired fish reproduction. The present study used a 21-d fathead minnow (Pimephales promelas) reproduction assay to investigate the reproductive toxicity of fipronil (FIP), a broad-spectrum phenylpyrazole insecticide that acts as a noncompetitive blocker of GABA receptor-gated chloride channels. Continuous exposure up to 5 µg FIP/L had no significant effect on most of the endpoints measured, including fecundity, secondary sexual characteristics, plasma steroid and vitellogenin concentrations, ex vivo steroid production, and targeted gene expression in gonads or brain. The gonad mass, gonadosomatic index, and histological stage of the gonad were all significantly different in females exposed to 0.5 µg FIP/L compared with those exposed to 5.0 µg FIP/L; however, there were no other significant effects on these measurements in the controls or any of the other treatments in either males and females. Overall, the results do not support a hypothesized adverse outcome pathway linking FIP antagonism of the GABA receptor(s) to reproductive impairment in fish.

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“…The relationship between the increase of DNA damage level in comet assay in this study and the increase in ROS level obtained in the study performed by Graillot is redolent of oxidative damage of DNA. Besides, Clasen et al (2012) indicated that FP has potential toxic effects on nontarget organisms. They reported that FP insecticides cause alterations in the biochemical parameters in different tissues, such as the liver and brain, of the common carp without affecting growth during the 90-day exposure period.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Genotoxicity of Fipronil Sister Chromatid Exchange, Cytokinesis Block Micronucleus Test, and Comet Assay

Çelık

Ekinci

Güler

et al. 2014

DNA and Cell Biology

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Fipronil (FP) is a phenylpyrazole pesticide developed by the transnational company Rhône-Poulenc Agro in 1987. Data on the genotoxicity and toxicity of FP are rather inadequate. In this study, we aimed to evaluate the potential genotoxic activity of FP using the single-cell microgel electrophoresis or comet assay, sister chromatid exchanges (SCEs), and micronuclei (MN) in human peripheral blood lymphocytes. In addition, the cytokinesis block proliferation index (CBPI) and proliferation index (PRI) were measured for cytotoxicity. In this study, three different doses of FP were used (0.7, 0.3, 0.1 μg/mL). Mitomycin C (2 μg/mL) and hydrogen peroxide were used as positive controls for SCE MN test systems, and comet assay, respectively. FP induced a statistically significant increase in the MN and SCE frequency and DNA damage in a dose-dependent manner in human peripheral blood lymphocytes (p<0.01, p<0.05, for 0.7 and 0.3 μg/mL, respectively) compared with a negative control. There is no significant difference between 0.1 μg/mL and the negative control for MN frequency, but there is significant difference between all the doses of FP and negative control for SCE frequency, mitotic index, CBPI, and PRI values (p<0.01). Using the alkaline comet assay, we showed that all the doses of the FP induced DNA damage in human peripheral blood lymphocytes in vitro (p<0.05).

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Effects of the commercial formulation containing fipronil on the non-target organism Cyprinus carpio: Implications for rice−fish cultivation

Cited by 76 publications

References 40 publications

Oxidative stress markers in fish (Astyanax sp. and Danio rerio) exposed to urban and agricultural effluents in the Brazilian Pampa biome

Oxidative stress markers in fish (Astyanax sp. and Danio rerio) exposed to urban and agricultural effluents in the Brazilian Pampa biome

Effects of the insecticide fipronil on reproductive endocrinology in the fathead minnow

In Vitro Genotoxicity of Fipronil Sister Chromatid Exchange, Cytokinesis Block Micronucleus Test, and Comet Assay

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