Effects of testosterone supplementation on prevention of age-related penile remodeling

Abstract

Erectile dysfunction develops among 46.2% of men between 40 and 70 years. Studies demonstrated substitution on detrusor muscle by collagen due testosterone deprivation. It is clear the correlation among aging and oxidative stress, accelerating apoptosis process in many tissues. This study aims to demonstrate the collagen substitution over the muscle fibers on muscle structure of rat's penis and the effects of testosterone supplementation. Sixteen senescent Wistar rats were divided into two groups: treatment (receiving standard supplementation testosterone dose) and control (receiving equivalent saline solution). Testosterone was dosed on D0 and D56 of study. All penises were prepared with picrosirius colored histology; stereology was applied to determine the volumetric density of collagen fibers (Vv). Analysis of variance demonstrated testosterone group's replacement therapy to be effective, while the androgenic decline continued by the time of experiment in control group (p < 0.05). Testosterone group had Vv of 20.6%, lower than control group (47.8%); t-test (p < 0.001). Pearson's correlation demonstrated an inverse correlation between the Vv and testosterone's levels (p < 0.001). This is a pioneer study on demonstration of structural alterations over the cavernous corpora muscle caused by deprivation of testosterone on elderly rat. These finding implicate that the testosterone levels can influence, not only the libido, but also the erectile function.

Clinical and preclinical treatment of urologic diseases with phosphodiesterase isoenzymes 5 inhibitors: an update

“…Given the important role of testosterone (T) in the sexual activity and decreased plasma level of T in unresponsive subjects, interest in T plus PDE5-Is therapy has increased in recent years. SM cell degeneration (apoptosis), adipose tissue and collagen fibers deposition in CC, and reduced expression of eNOS and neural nitric oxide synthase (nNOS) were confirmed in hypogonadism subjects by previous studies 43444546. Upregulation of CCSM contractility was also found in castrated or diabetic animal models 47484950.…”

Translational Perspective on the Role of Testosterone in Sexual Function and Dysfunction

“…Very limited basic science studies have evaluated the impact of aging-associated androgen deficiency on sexual function. Treatment of old (20–22-month) rats with testosterone and DHT for 1.5–2 months was found to improve sexual behavior (mount rate) [118] erectile function [117], and decrease collagen content in the penis [33]. On the other hand, no improvement in erectile function of old rats by testosterone replacement has been noted [119].…”

Estrogen and testosterone supplementation improves tendon healing and functional recovery after rotator cuff repair

“…For testosterone supplementation we used pharmaceutical grade testosterone undecanoate, which is an ester of testosterone that has a half‐life of 20 days when injected intramuscularly. It has been previously used in rodents at doses between 38 and 50 mg/kg 24–26 . We injected 40 mg/kg (0.05 mL) testosterone undecanoate intramuscularly beginning on the day of surgery and provided a second dose 28 days later.…”

“…It has been previously used in rodents at doses between 38 and 50 mg/kg. [24][25][26] We injected 40 mg/kg (0.05 mL) testosterone undecanoate intramuscularly beginning on the day of surgery and provided a second dose 28 days later.…”