Effects of Tempol and L-Ergothioneine on motility parameters of cryopreserved stallion sperm

Different concentrations of cysteamine, ergothioneine, and serine modulate quality and fertilizing ability of cryopreserved chicken sperm

“…ERG is an important component of seminal plasma in many mammals where it is expected to protect sperm from oxidative stress, to counteract the effects of peroxides on sperm viability, and to enhance the viability of sperm during storage ( Mann and Leone, 1953 ; Haag and McLeod, 1959 ). Addition of ERG in the diluent improved the post-thawed motility in ram ( Ҫoyan et al., 2011 , Ari et al., 2012 , Çoyan et al., 2012 , Najafi et al., 2014 ) and stallion ( Coutinho de Silva et al., 2008 , Metcalf et al., 2008 ). However, some reports failed to show the antioxidant action of ERG on goat semen conservation ( Bucak et al., 2007 , Bucak et al., 2009 , Ҫoyan et al., 2011 ).…”

“…Its role is to protect sperm from oxidative stress, given the exceptionally high metabolic rate, and counteracts the deleterious effects of peroxides on sperm viability and survival during storage ( Mann and Leone, 1953 , Haag and McLeod, 1959 ). ERG improves sperm DNA integrity during short storage at 5°C and frozen-thawed motility in ram and stallion ( Coutinho de Silva et al., 2008 , Metcalf et al., 2008 , Ҫoyan et al., 2011 , Ari et al., 2012 , Çoyan et al., 2012 , Najafi et al., 2014 ).…”

Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender

“…Ergothioneine has also been used for freezing stallion semen, with mixed results. Coutinho da Silva et al [15], obtained no improvement of sperm motility, whereas Metcalf et al [41], found that ergothioneine enhanced post-thaw semen quality in some instances. Bucak et al [9], added cysteamine to a goat extender also containing egg yolk, reporting that cysteamine at 5 mM improved goat sperm motility post-thawing, but at 10 mM it decreased motility.…”