Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender

Najafi, Abouzar; Kia, Hossein Daghigh; Mohammadi, Hossein; Najafi, M.H.; Zanganeh, Zaynab; Sharafi, Mohsen; Martínez‐Pastor, Felipe; Adeldust, Hamideh

doi:10.1016/j.cryobiol.2014.05.004

Cited by 53 publications

(28 citation statements)

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“…However, the feasibility and the quality of the produced ovine embryos are lower than for other species (Romao et al, 2013).This is mainly because ram spermatozoa are sensitive to the conditions of lowtemperature anabiosis and freezing (Sabev et al, 2006). Although semen possesses an antioxidant system, cryopreservation increases the intensity of lipid peroxidation of spermatozoa and their activity is affected (Najafi et al, 2014). Previous results showed that supplementation with antioxidants such as cysteamine, ergothioneine, vitamin E analogue, Trolox, catalase, and superoxide dismutase mimetic agent reduces lipid peroxidation and H 2 O 2 generation (Forouzanfar et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Research has shown that the antioxidant activity of polyphenols is more effective and less toxic than vitamins E and C. However, various factors, such as method of administration, dose, and structure of phenolic compounds, affect their activities (Silva et al, 2012). For example, antioxidants might have negative effects owing to excessive scavenging of free radicals, possibly by altering their physiological levels (Najafi et al, 2014). As shown in the current study, viability, motility and fertility of spermatozoa decreased significantly when higher concentrations of polyphenol quercetin (≥20 µg/mL) were used.…”

Section: Discussionmentioning

confidence: 99%

“…The female size, low economic return, variable fertility rates, and more rapid loss of semen quality with storage time are important obstacles for the routine use of these techniques. Therefore, sperm quality, which is negatively affected by cryopreservation, has a critical impact on the application of AI (Mata-Campuzano et al, 2014;Najafi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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The effect of quercetin on fertility of frozen-thawed ram epididymal spermatozoa

2017

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The aim of the present study was to evaluate the effects of quercetin as an antioxidant supplement on frozen-thawed ram epididymal sperm quality. Quercetin is a type of flavonoid antioxidant that is found in plants, with the ability to scavenge free radicals. Twenty testicles from mature rams were collected from a nearby slaughterhouse immediately after slaughter. Epididymal spermatozoa were recovered from the caudal of epididymides by injecting Bracket and Oliphant's (BO) medium retrogradely through the ductus deferens and extended with a tris egg-yolk-based extender and supplemented with 0, 5, 10, 15, 20, and 50 µg/mL quercetin. Following equilibration, the straws were frozen, and then plunged into liquid nitrogen. After thawing, optimized concentrations of quercetin were defined based on their viabilities and used to assess fertilization and developmental potential. The results showed that the viability of frozen-thawed spermatozoa significantly increased by using 5 and 10 µg/mL quercetin in the freezing extender. However, total and progressive motility of frozen-thawed spermatozoa were not affected by 5 and 10 µg/mL quercetin in comparison with control (0 µg/mL). The mean number of zygote, morula, and blastocyst stage embryos increased significantly by using 5 and 10 µg/mL quercetin compared with other frozen-thawed treatments(P <0.05). However, the blastocyst rate of fresh sperm was significantly higher (P <0.05). In conclusion, to improve the quality of frozen-thawed ram epididymal spermatozoa, 5 and 10 µg/mL quercetin appears to be an attractive option. Further studies are suggested to understand the synergistic effect of quercetin with other antioxidants to improve the ram freezing-thawing process.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of quercetin on fertility of frozen-thawed ram epididymal spermatozoa

2017

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“…Esto se ve reflejado en la reducción de la movilidad, la viabilidad y la capacidad fecundante de dichas células (Najafi et al 2014). Sin embargo, los espermatozoides de asno han demostrado ser resistentes a los procesos de refrigeración y criopreservación, al mantener características adecuadas de calidad seminal (Vilés-López, 2013).…”

Section: Introductionunclassified

Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación

2017

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Palabras clave adicionalesCalidad seminal. Crioprotectores. Plasma seminal. Yema de huevo. Criopreservación. resUMenLa congelación de semen de asno permite almacenar material genético por un periodo ilimitado de tiempo, por lo que puede ser empleado para generar bancos de germoplasma o para la cría de mulares y asnos. Esta investigación tuvo como objetivo evaluar alternativas de suplementación de un diluyente para la congelación de semen de asno. Se colectó el semen de cinco asnos criollos colombianos, dos eyaculados por animal (n= 10) y luego se congeló en diluyente Equiplus® suplementado con 3% de glicerol (GLY) ó 5% de dimetilformamida (DMF), 2% ó 5% de yema de huevo centrifugada (YHC) y 10% ó 20% de plasma seminal (PS). Se realizó la evaluación post-descongelación de la calidad seminal y de la peroxidación lipídica (PL). Para el análisis estadístico se ajustaron modelos mixtos, se realizó un análisis de regresión y comparaciones de medias por la prueba de Tukey. El semen congelado con 5% de DMF, 5% de YHC y 10% de PS presentó valores superiores de movilidad, vitalidad e integridad de membrana (p<0,05). La PL fue menor en diluyentes con 5% de YHC (p<0,05). La suplementación del diluyente de congelación con una combinación de 5% de DMF, 5% de YHC y 10% de PS permite mejorar la calidad post-descongelación del semen de asno. Effect of extender supplementation on the post-thaw semen quality of donkey sUMMarY Freezing of donkey semen allows storing genetic material for an unlimited period of time, which can be used to generate germoplasm banks or for breeding of mules or asses. This research aimed to evaluate supplementation alternatives for freezing donkey semen. Semen from five Colombian creole donkeys (n= 10) was collected and then frozen in Equiplus® extender supplemented with 3% of glycerol (GLY) or 5% of dimethylformamide (DMF), 2% or 5% of centrifuged egg yolk (CEY) and 10% or 20% of seminal plasma (SP). Post-thaw evaluation of semen quality and lipid peroxidation (LP) was conducted. For statistical analysis mixed models were adjusted, regression analysis and comparison of means by the Tukey test were performed. Frozen semen with DMF, 5% CEY and 10% SP presented superior results of motility and membrane integrity (p<0.05). The LP was lower in extenders with 5% CEY (p<0.05). Supplementation of freezing extender with the combination of 5% DMF, 5% CEY and 10% SP allows to improve the post-thaw quality of asinine semen.additional keYwords

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“…Semen cryopreservation has allowed specific opportunities for conservation of genetic resources through sperm banks, guarantee of a constant commercial supply of semen, and collaboration in breed improvement programs by artificial insemination (Bucak et al, 2007;Masoudi et al, 2017). Semen cryopreservation may lead to oxidative, chemical and physical damages on sperm membrane leading to reduction of sperm viability and fertility (Evans et al, 1987;Watson, 2000;Emamverdi et al, 2014;Najafi et al, 2014a;Sarıözkan et al, 2015). Freezing process mostly leads to loss of motility, acrosomal and plasma membrane functionality of spermatozoa (Tuncer et al, 2011;Najafi et al, 2013;Shahverdi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen

Motemani

Chamani

Sharafi

et al. 2017

Span. j. agric. res.

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This study was conducted to investigate the effects of different levels of α-tocopherol in cryopreservation media on the bull sperm quality after thawing. Semen samples were collected from six Holstein bulls using artificial vagina twice a week. Semen samples were pooled to eliminate individual differences and then divided into four equal parts for freezing with extenders containing different concentrations of α-tocopherol according to experimental groups as follows: 0 (control), 1.2 mM (E1), 2.4 mM (E2) and 4.8 mM (E4). Motion characteristics, viability, plasma membrane functionality, lipid peroxidation and H 2 O 2 status were determined after thawing. Results showed that malondialdehyde (MDA) concentration was significantly lower in E4 (6.1±0.6 nmol/mL) than E1 and E2 extenders (8.1±0.6 nmol/mL and 8±0.6 nmol/mL, respectively). Also, the lowest significant concentration of H 2 O 2 was observed in E4 (3.2±0.13 nmol/mL) compared to E1 (4 ±0.1 nmol/mL), E2 (5.3± 0.1 nmol/mL) and control (6.7±0.1 nmol/mL). Moreover, E2 and E4 produced the highest significant motility (74.2±1.6%, 75.9±1.6%), viability (78.2±1.8%, 76.1±1.8%) and membrane functionality (73±1.6%, 70.5±1.6%) compared to other groups. It can be concluded that α-tocopherol at the concentration of 4.8 mM can be an efficient antioxidant additive in Bioxcell extender for cryopreservation of bull semen.Additional key words: antioxidant; bioxcell; lipid peroxidation; sperm; H 2 O 2 .Correspondence should be addressed to Mohammad Chamani: m.chamani@srbiau.ac.ir Abbreviations used: LPO (lipid peroxidation); MDA (malondialdehyde); ROS (reactive oxygen species).Funding: The authors received no specific funding for this work.

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Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender

Cited by 53 publications

References 54 publications

The effect of quercetin on fertility of frozen-thawed ram epididymal spermatozoa

The effect of quercetin on fertility of frozen-thawed ram epididymal spermatozoa

Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación

Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen

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