Effects of Temperature on Composition and Cell Volume of
            <i>Candida utilis</i>

Brown, C. M.; Rose, Anthony H.

doi:10.1128/jb.97.1.261-272.1969

Cited by 80 publications

(21 citation statements)

References 34 publications

(25 reference statements)

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“…This increased RNA was probably ribosomal RNA, as it generally dominates the cellular RNA pool (65-81% cellular RNA; Neidhardt et al 1990;Lehninger, Nelson & Cox 1993) and increases in proportion to other types of RNA with increasing growth rate (Neidhardt et al 1990). Similar trends of growth-dependent variation in RNA content have also been observed in cyanobacteria (Binder & Liu 1998;Lepp & Schmidt 1998); yeast (Brown & Rose 1969;Aiking & Tempest 1976); and freshwater eukaryotic algae (Rhee 1978). Within the range of growth rates that we applied, the RNA and DNA contents in our experiments were similar to those of E. coli recorded by Jacobsen (1974; cited by Neidhardt et al 1990) and the E. coli RNA : DNA ratios were almost identical to those reported by Skjold, Juarez & Hedgcoth (1973) and of freshwater and enterobacteria summarized by Dortch et al (1983).…”

Section:         -supporting

confidence: 87%

Are bacteria more like plants or animals? Growth rate and resource dependence of bacterial C : N : P stoichiometry

Makino¹,

Cotner²,

Sterner³

et al. 2003

Functional Ecology

331

262

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Summary 1.We examined the relative importance of resource composition (carbon : phosphorus molar ratios which varied between 9 and 933) and growth rate (0·5-1·5 h − 1 ) to biomass carbon : nitrogen : phosphorus stoichiometry and nucleic acid content in Escherichia coli grown in chemostats, and in other heterotrophic prokaryotes using published literature. 2. Escherichia coli RNA content and the contribution of RNA-P to total cellular P increased with increasing growth rate at all supply C : P ratios. Growth rate had a much stronger effect on biomass C : P than did supply C : P, and increased RNA content resulted in low biomass C : P and N : P ratios. 3. However, we observed only twofold variations in biomass C : P and N : P ratios in the experiments, despite a difference of two orders of magnitude in C : P and N : P supply. The response of biomass C : P and N : P ratios to alteration of the supply C : P and N : P ratios revealed that E. coli was strongly homeostatic in its elemental composition. 4. This result, and a literature survey, suggest that each heterotrophic bacterial strain regulates its elemental composition homeostatically within a relatively narrow range of characteristic biomass C : P and N : P ratios. 5. Thus shifts in the dominance of different bacterial strains in the environment are probably responsible for the large variation in bacterial biomass C : P, as has been suggested for crustacean zooplankton. These findings indicate that bacteria are more like animals than plants in terms of biomass C : P and N : P homeostasis.

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Section:         -supporting

confidence: 87%

Are bacteria more like plants or animals? Growth rate and resource dependence of bacterial C : N : P stoichiometry

Makino¹,

Cotner²,

Sterner³

et al. 2003

Functional Ecology

331

262

View full text Add to dashboard Cite

show abstract

“…The greater the nutrient limitation, the larger the decrease of temperature required for growth to be controlled purely by temperature. Brown and Rose (1969) apparently found a similar relationship in C. utilis; they observed that the temperature at which the population washed out of a chemostat vessel decreased with decreasing dilution rate.…”

Section: Discussionmentioning

confidence: 67%

“…To increase RNA synthesis, more protein or enzymes may also be needed. The increased RNA in Aerobacter aerogenes (Tempest and Hunter 1965) and Candida utilis (Brown and Rose 1969) was accounted for by ribosomal RNA. The decreased yield (l/y) at lower temperatures in our work may also be due to high maintenance requirement of energy (Tempest and Hunter 1965).…”

Section: Discussionmentioning

confidence: 99%

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The effect of environmental factors on phytoplankton growth: Temperature and the interactions of temperature with nutrient limitation1

Rhee

Gotham

1981

Limnology & Oceanography

329

214

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The combined stress of nutrient limitation and suboptimal temperature on growth was studied with turbidostat and chemostat cultures of Scenedesmus sp. and Asterionella formosa. The combined effects were greater than the sum of individual effects and were not multiplicative.In N-and P-limited Scenedesmus sp. and A. formosa the cell quotas (4) of both limiting and nonlimiting nutrients increased with decreasing temperature. At a given temperature cell quotas of limiting nutrients ,also increased with the growth rate (p) and followed a saturation function. Higher values of the minimum cell quota (9J at lower temperatures show that cells require more nutrient with decreasing temperature.The change of q,, with temperature varies with the type of limiting nutrient. This change for N and P in Scenedesmus sp. suggests that their optimum ratio, the ratio at which one limitation changes over to the other, is higher at suboptimal temperatures.Cell quotas of nutrient-sufficient cultures (qm) for C, N, and P and cellular chlorophyll a concentration increased with decreasing temperature. The quota of each nonlimiting nutrient in nutrient-limited cultures had the same value as 9m. RNA decreased with temperature.

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“…Cell fractionation and analyses. Cells harvested from basal (control cells) and supplemented media (AMcells) were fractionated by the methods of Brown and Rose (2). Total carbohydrate was measured by the anthrone method (8) with D-glucose as standard.…”

mentioning

confidence: 99%

Modification of the Composition and Structure of the Yeast Cell Wall by Culture in the Presence of Sulfur Amino Acids

Killick

1971

J Bacteriol

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Growth of Candida utilis and Saccharomyces cerevisiae in a medium supplemented with sulfur amino acids led to synthesis and accumulation of S-adenosylmethionine, accompanied by a reduction in the cell yield, an increased sensitivity of the cell wall to snail gut enzymes (Helix pomatia), as judged by spheroplast formation, and by a modification of the chemical composition of both the intact cells and their isolated walls. Walls of supplemented cultures of C. utilis were three times as sensitive to enzymatic digestion as walls from nonsupplemented cultures. In contrast to C. utilis, walls isolated from supplemented cultures of S. cerevisiae were digested slightly more rapidly by the purified snail extract than those from nonsupplemented cultures. Chemical modifications of the cell wall are interpreted to explain the ease with which cells from sulfur amino acid-supplemented cultures are converted to spheroplasts.Jlized by membrane filtration (type HA, 0.45 ,um pore size; Millipore Corp., Bedford, Mass.) 931 on July 10, 2020 by guest

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Effects of Temperature on Composition and Cell Volume of Candida utilis

Cited by 80 publications

References 34 publications

Are bacteria more like plants or animals? Growth rate and resource dependence of bacterial C : N : P stoichiometry

Are bacteria more like plants or animals? Growth rate and resource dependence of bacterial C : N : P stoichiometry

The effect of environmental factors on phytoplankton growth: Temperature and the interactions of temperature with nutrient limitation1

Modification of the Composition and Structure of the Yeast Cell Wall by Culture in the Presence of Sulfur Amino Acids

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