Effects of Tea Polyphenols on the Invasion and Matrix Metalloproteinases Activities of Human Fibrosarcoma HT1080 Cells

Maeda‐Yamamoto, Mari; Kawahara, Hiroharu; Tahara, Nobufumi; Tsuji, Kenkou; Hara, Yukihiko; Isemura, Mamoru

doi:10.1021/jf9811525

Cited by 151 publications

(93 citation statements)

References 29 publications

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“…1 The latter two also exert inhibitory activity toward the two gelatinases, but whereas EGC is effective at concentrations 20 -30 times higher than EGCG, ECG has been reported to block gelatinases at concentrations close to those registered here for EGCG. 11 This fits with the suggestion that both the flavanol skeleton and the galloyl moiety are necessary for the inhibitory activity. In fact, gallic acid itself lacks any in-gel inhibitory activity on gelatinases in the same range of concentration, but tannic acid (gallotannin) shows an even stronger inhibition than EGCG; nevertheless, although the gallotannin-gelatinase interaction leads to enzyme precipitation, in presence of the flavanol the gelatinase remains in solution.…”

Section: Discussionsupporting

confidence: 86%

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Tumor gelatinases and invasion inhibited by the green tea flavanol epigallocatechin-3-gallate

Garbisa

Sartor

Biggin

et al. 2001

Cancer

291

199

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Section: Discussionsupporting

confidence: 86%

“…From this, it was a short step to suspect that EGCG may be a direct inhibitor of MMP-2 and MMP-9 gelatinases; soon after, this was confirmed in preliminary studies. 11,12 However, the molecular mechanisms responsible for this activity remain to be elucidated.…”

mentioning

confidence: 99%

Tumor gelatinases and invasion inhibited by the green tea flavanol epigallocatechin-3-gallate

Garbisa

Sartor

Biggin

et al. 2001

Cancer

291

199

View full text Add to dashboard Cite

“…28 Inhibition of MMPs has also been reported in several studies of phenolics compounds from green tea. [29][30][31] The reduction in total cell number in the Matrigel invasion assay was not due to cytotoxicity, as demonstrated in cell viability study (data not shown).…”

Section: Discussionmentioning

confidence: 77%

Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro

Hashim

Rowland

McGlynn

et al. 2007

Intl Journal of Cancer

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Studies in human, animal and cellular systems suggest that phenols from virgin olive oil are capable of inhibiting several stages in carcinogenesis, including metastasis. The invasion cascade comprises cell attachment to extracellular matrix components or basement membrane, degradation of basement membrane by proteolytic enzymes and migration of cells through the modified matrix. In the present study, we investigated the effect of phenolics extracted from virgin olive oil (OVP) and its main constituents: hydroxytyrosol (3,4-dihydroxyphenylethanol), tyrosol (p-hydroxyphenylethanol), pinoresinol and caffeic acid. The effects of these phenolics were tested on the invasion of HT115 human colon carcinoma cells in a Matrigel invasion assay. OVP and its compounds showed different dose-related anti-invasive effects. At 25 lg/ml OVP and equivalent doses of individual compounds, significant anti-invasive effects were seen in the range of 45-55% of control. Importantly, OVP, but not the isolated phenolics, significantly reduced total cell number in the Matrigel invasion assay. There were no significant effects shown on cell viability, indicating the reduction of cell number in the Matrigel invasion assay was not due to cytotoxicity. There were also no significant effects on cell attachment to plastic substrate, indicating the importance of extracellular matrix in modulating the anti-invasive effects of OVP. In conclusion, the results from this study indicate that phenols from virgin olive oil have the ability to inhibit invasion of colon cancer cells and the effects may be mediated at different levels of the invasion cascade. ' 2007 Wiley-Liss, Inc.Key words: olive oil phenolics; colorectal cancer; invasion; adhesion; extracellular matrix In 2002, world cancer statistics for new colorectal cancer (CRC) cases were estimated to be more than 1 million, with more than 500,000 mortality cases. 1 Surgical resection is an effective treatment for localized disease, achieving a 5-year survival rate of 90%, but other normal treatments for metastatic disease remain ineffective. 2 Mediterranean countries have lower rates of CRC than other Western countries. 3 This has been attributed to a number of factors, one of which is consumption of olive oil. 3,4 Recently, olive oil phenolics have been shown to exert anticancer effects in a number of studies (reviewed in Refs. 5 and 6). Our previous study demonstrated that olive oil phenolics have the ability to inhibit initiation, promotion and invasion events in in vitro models of carcinogenesis. 7 Metastasis can be considered as a cascade of interrelated sequential steps. Cells must be able to disseminate from the primary tumor, invade the surrounding tissue, enter the circulatory system, evade immune responses, arrest at and colonize a distant site. 8 A 3-step hypothesis has been proposed to describe invasion of tumor cells: attachment to basement membrane or extracellular matrices (ECM), protease activity that induces local degradation of the matrix, and migration of tumor cells through the...

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“…The first pathway involves direct inhibition of MMP-2 and MMP-9 enzymatic activity (19). Tea polyphenols can form complexes with metal ions (56). As a result, TFDG and EGCG may inhibit MMPs activity by forming complexes with zinc ions; these complexes will inhibit MMPs as MMPs are zincdependent proteinases.…”

Section: Figmentioning

confidence: 99%

Tea Polyphenols Inhibit Rat Osteoclast Formation and Differentiation

et al. 2012

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Abstract. Matrix metalloproteinases (MMPs) play an important role in degeneration of the matrix associated with bone and cartilage. Regulation of osteoclast activity is essential in the treatment of bone disease, including osteoporosis and rheumatoid arthritis. Polyphenols in green tea, particularly epigallocatechin-3-gallate (EGCG), inhibit MMPs expression and activity. However, the effects of the black tea polyphenol, theaflavin-3,3′-digallate (TFDG), on osteoclast and MMP activity are unknown. Therefore, we examined whether TFDG and EGCG affect MMP activity and osteoclast formation and differentiation in vitro. TFDG or EGCG (10 and 100 μM) was added to cultures of rat osteoclast precursors cells and mature osteoclasts. Numbers of multinucleated osteoclasts and actin rings decreased in polyphenol-treated cultures relative to control cultures. MMP-2 and MMP-9 activities were lower in TFDG-and EGCG-treated rat osteoclast precursor cells than in control cultures. MMP-9 mRNA levels declined significantly in TFDG-treated osteoclasts in comparison to control osteoclasts. TFDG and EGCG inhibited the formation and differentiation of osteoclasts via inhibition of MMPs. TFDG may suppress actin ring formation more effectively than EGCG. Thus, TFDG and EGCG may be suitable agents or lead compounds for the treatment of bone resorption diseases.

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Effects of Tea Polyphenols on the Invasion and Matrix Metalloproteinases Activities of Human Fibrosarcoma HT1080 Cells

Cited by 151 publications

References 29 publications

Tumor gelatinases and invasion inhibited by the green tea flavanol epigallocatechin-3-gallate

Tumor gelatinases and invasion inhibited by the green tea flavanol epigallocatechin-3-gallate

Inhibitory effects of olive oil phenolics on invasion in human colon adenocarcinoma cells in vitro

Tea Polyphenols Inhibit Rat Osteoclast Formation and Differentiation

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